Vanadate stimulates rat pancreatic enzyme secretion through the release of calcium from an intracellular store
Orthovanadate accelerates 45Ca efflux and enzyme secretion from the rat pancreas incubated in either control (2.5 mM Ca) or nominally Ca-free buffers. Secretion induced by vanadate does not appear to be mediated by changes in either adenylate cyclase or sodium pump activity. Instead, vanadate appear...
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| Veröffentlicht in: | Cell calcium (Edinburgh) 1984-08, Vol.5 (4), p.335-350 |
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| creator | Proffitt, R. Case, R.M. |
| description | Orthovanadate accelerates
45Ca efflux and enzyme secretion from the rat pancreas incubated in either control (2.5 mM Ca) or nominally Ca-free buffers. Secretion induced by vanadate does not appear to be mediated by changes in either adenylate cyclase or sodium pump activity. Instead, vanadate appears to act at an intracellular site to cause the release of calcium from the same pool mobilised by acetylcholine. Vanadate action is not inhibited by DIDS. The effect of pH on vanadate action may be accounted for by changes in the distribution of the vanadates. Vanadyl sulphate inhibits secretion evoked by acetylcholine. This suggests that intracellular reduction of vanadate (+5 oxidation state) to the +4 oxidation state may account for an inhibitory component observed during stimulation with vanadate. |
| doi_str_mv | 10.1016/0143-4160(84)90002-2 |
| format | Article |
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45Ca efflux and enzyme secretion from the rat pancreas incubated in either control (2.5 mM Ca) or nominally Ca-free buffers. Secretion induced by vanadate does not appear to be mediated by changes in either adenylate cyclase or sodium pump activity. Instead, vanadate appears to act at an intracellular site to cause the release of calcium from the same pool mobilised by acetylcholine. Vanadate action is not inhibited by DIDS. The effect of pH on vanadate action may be accounted for by changes in the distribution of the vanadates. Vanadyl sulphate inhibits secretion evoked by acetylcholine. This suggests that intracellular reduction of vanadate (+5 oxidation state) to the +4 oxidation state may account for an inhibitory component observed during stimulation with vanadate.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</subject><subject>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives</subject><subject>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology</subject><subject>Acetylcholine - pharmacology</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calmodulin - antagonists & inhibitors</subject><subject>Exocrine pancreas</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ouabain - pharmacology</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - enzymology</subject><subject>Potassium - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sodium - metabolism</subject><subject>Vanadates</subject><subject>Vanadium - pharmacology</subject><subject>Vertebrates: digestive system</subject><issn>0143-4160</issn><issn>1532-1991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo6-zqP1DIQUQPrfnsTi6CLK4KC17Ua6hOV7uR7mRM0sL66804wxw9VVH11EvxEPKMszec8f4t40p2ivfslVGvLWNMdOIB2XEtRcet5Q_J7ow8Jpel_GyMlQO_IBe9YIMVZkfid4gwQUVaali3pXWFZqh0D9FnhBo8xfjnfm0AtkENKdJ6l9P2465VpBkXhII0zdTD4sO20jmnlUKkIdYMHpelxeaWnzI-IY9mWAo-PdUr8u3mw9frT93tl4-fr9_fdl6avnZSaqGE13o0ABYnDl6C7cehV4LxibNBGzBtrP04AkxGgNJi9qDGgQ9mklfk5TF3n9OvDUt1ayiHVyBi2oozXFjDBtlAdQR9TqVknN0-hxXyvePMHTS7g0N3cOiMcv80O9HOnp_yt3HF6Xx08tr2L057KE3LnJvNUM6YZVpbqRv27ohhc_E7YHbFB4wep5DRVzel8P8__gL0iZqw</recordid><startdate>198408</startdate><enddate>198408</enddate><creator>Proffitt, R.</creator><creator>Case, R.M.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198408</creationdate><title>Vanadate stimulates rat pancreatic enzyme secretion through the release of calcium from an intracellular store</title><author>Proffitt, R. ; Case, R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-335242c55b8aa9ed1ac3a96b764201d10758a8d1a5cbbaad82a452fca4b7178d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</topic><topic>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives</topic><topic>4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology</topic><topic>Acetylcholine - pharmacology</topic><topic>Adenylyl Cyclases - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calmodulin - antagonists & inhibitors</topic><topic>Exocrine pancreas</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Ouabain - pharmacology</topic><topic>Pancreas - drug effects</topic><topic>Pancreas - enzymology</topic><topic>Potassium - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sodium - metabolism</topic><topic>Vanadates</topic><topic>Vanadium - pharmacology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Proffitt, R.</creatorcontrib><creatorcontrib>Case, R.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell calcium (Edinburgh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Proffitt, R.</au><au>Case, R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vanadate stimulates rat pancreatic enzyme secretion through the release of calcium from an intracellular store</atitle><jtitle>Cell calcium (Edinburgh)</jtitle><addtitle>Cell Calcium</addtitle><date>1984-08</date><risdate>1984</risdate><volume>5</volume><issue>4</issue><spage>335</spage><epage>350</epage><pages>335-350</pages><issn>0143-4160</issn><eissn>1532-1991</eissn><coden>CECADV</coden><abstract>Orthovanadate accelerates
45Ca efflux and enzyme secretion from the rat pancreas incubated in either control (2.5 mM Ca) or nominally Ca-free buffers. Secretion induced by vanadate does not appear to be mediated by changes in either adenylate cyclase or sodium pump activity. Instead, vanadate appears to act at an intracellular site to cause the release of calcium from the same pool mobilised by acetylcholine. Vanadate action is not inhibited by DIDS. The effect of pH on vanadate action may be accounted for by changes in the distribution of the vanadates. Vanadyl sulphate inhibits secretion evoked by acetylcholine. This suggests that intracellular reduction of vanadate (+5 oxidation state) to the +4 oxidation state may account for an inhibitory component observed during stimulation with vanadate.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>6207928</pmid><doi>10.1016/0143-4160(84)90002-2</doi><tpages>16</tpages></addata></record> |
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| identifier | ISSN: 0143-4160 |
| ispartof | Cell calcium (Edinburgh), 1984-08, Vol.5 (4), p.335-350 |
| issn | 0143-4160 1532-1991 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology Acetylcholine - pharmacology Adenylyl Cyclases - metabolism Animals Biological and medical sciences Calcium - metabolism Calmodulin - antagonists & inhibitors Exocrine pancreas Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Ouabain - pharmacology Pancreas - drug effects Pancreas - enzymology Potassium - metabolism Rats Rats, Inbred Strains Sodium - metabolism Vanadates Vanadium - pharmacology Vertebrates: digestive system |
| title | Vanadate stimulates rat pancreatic enzyme secretion through the release of calcium from an intracellular store |
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