Techniques for preparation of bovine mammary smooth membranes
Most procedures for fractionation of lactating mammary tissue membranes have utilized centrifuge tubes of small volume. We report a method for large-scale fractionation of bovine mammary membranes by a zonal rotor. Because of the large volume of the zonal rotor (1350 ml), greater amounts of starting...
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| Veröffentlicht in: | Journal of dairy science 1984-09, Vol.67 (9), p.2055-2061 |
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| container_title | Journal of dairy science |
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| creator | Pocius, P. A Dreels, J. M Devery-Pocius, J. E Baumrucker, C. R |
| description | Most procedures for fractionation of lactating mammary tissue membranes have utilized centrifuge tubes of small volume. We report a method for large-scale fractionation of bovine mammary membranes by a zonal rotor. Because of the large volume of the zonal rotor (1350 ml), greater amounts of starting material can be fractionated to obtain greater yields of desired fractions. We report results of linear sucrose gradients to fractionate mammary tissue homogenates and step gradient adaptations to accelerate membrane separations. Assay of membrane fractions for marker enzyme activities demonstrated that zonal fractionation and derived techniques were applicable for large scale preparation with higher yields of selected bovine mammary membranes. Application of this technique to small volumes is discussed for laboratories without a zonal rotor. |
| doi_str_mv | 10.3168/jds.S0022-0302(84)81544-1 |
| format | Article |
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A ; Dreels, J. M ; Devery-Pocius, J. E ; Baumrucker, C. R</creator><creatorcontrib>Pocius, P. A ; Dreels, J. M ; Devery-Pocius, J. E ; Baumrucker, C. R ; Freie Univ. Berlin (Germany, F.R.). Fachbereich Veterinaermedizin</creatorcontrib><description>Most procedures for fractionation of lactating mammary tissue membranes have utilized centrifuge tubes of small volume. We report a method for large-scale fractionation of bovine mammary membranes by a zonal rotor. Because of the large volume of the zonal rotor (1350 ml), greater amounts of starting material can be fractionated to obtain greater yields of desired fractions. We report results of linear sucrose gradients to fractionate mammary tissue homogenates and step gradient adaptations to accelerate membrane separations. Assay of membrane fractions for marker enzyme activities demonstrated that zonal fractionation and derived techniques were applicable for large scale preparation with higher yields of selected bovine mammary membranes. Application of this technique to small volumes is discussed for laboratories without a zonal rotor.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(84)81544-1</identifier><identifier>PMID: 6149240</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Am Dairy Sci Assoc</publisher><subject>5'-Nucleotidase ; Acid Phosphatase - metabolism ; Animals ; aparato golgi ; appareil de golgi ; Biological and medical sciences ; Cattle - anatomy & histology ; Cell Membrane - enzymology ; Cell Membrane - ultrastructure ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Centrifugation, Zonal - methods ; cows ; Endoplasmic Reticulum - metabolism ; Female ; fraccionamiento ; fractionation ; fractionnement ; Fundamental and applied biological sciences. Psychology ; Galactosyltransferases - metabolism ; gamma-Glutamyltransferase - metabolism ; glande mammaire ; glandulas mamarias ; golgi apparatus ; Golgi Apparatus - enzymology ; Intracellular Membranes - ultrastructure ; Lysosomes - enzymology ; mammary glands ; Mammary Glands, Animal - ultrastructure ; Microscopy, Electron ; Mitochondria - enzymology ; Molecular and cellular biology ; NADPH-Ferrihemoprotein Reductase - metabolism ; Nucleotidases - metabolism ; Succinate Dehydrogenase - metabolism ; vaca ; vache</subject><ispartof>Journal of dairy science, 1984-09, Vol.67 (9), p.2055-2061</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-820eeb74749528b43e7c3dce8092c429eae15cc17655d1ec77092eb2addba88b3</citedby><cites>FETCH-LOGICAL-c474t-820eeb74749528b43e7c3dce8092c429eae15cc17655d1ec77092eb2addba88b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27876,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8895708$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6149240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pocius, P. A</creatorcontrib><creatorcontrib>Dreels, J. M</creatorcontrib><creatorcontrib>Devery-Pocius, J. E</creatorcontrib><creatorcontrib>Baumrucker, C. R</creatorcontrib><creatorcontrib>Freie Univ. Berlin (Germany, F.R.). Fachbereich Veterinaermedizin</creatorcontrib><title>Techniques for preparation of bovine mammary smooth membranes</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>Most procedures for fractionation of lactating mammary tissue membranes have utilized centrifuge tubes of small volume. We report a method for large-scale fractionation of bovine mammary membranes by a zonal rotor. Because of the large volume of the zonal rotor (1350 ml), greater amounts of starting material can be fractionated to obtain greater yields of desired fractions. We report results of linear sucrose gradients to fractionate mammary tissue homogenates and step gradient adaptations to accelerate membrane separations. Assay of membrane fractions for marker enzyme activities demonstrated that zonal fractionation and derived techniques were applicable for large scale preparation with higher yields of selected bovine mammary membranes. Application of this technique to small volumes is discussed for laboratories without a zonal rotor.</description><subject>5'-Nucleotidase</subject><subject>Acid Phosphatase - metabolism</subject><subject>Animals</subject><subject>aparato golgi</subject><subject>appareil de golgi</subject><subject>Biological and medical sciences</subject><subject>Cattle - anatomy & histology</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Centrifugation, Zonal - methods</subject><subject>cows</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Female</subject><subject>fraccionamiento</subject><subject>fractionation</subject><subject>fractionnement</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactosyltransferases - metabolism</subject><subject>gamma-Glutamyltransferase - metabolism</subject><subject>glande mammaire</subject><subject>glandulas mamarias</subject><subject>golgi apparatus</subject><subject>Golgi Apparatus - enzymology</subject><subject>Intracellular Membranes - ultrastructure</subject><subject>Lysosomes - enzymology</subject><subject>mammary glands</subject><subject>Mammary Glands, Animal - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Mitochondria - enzymology</subject><subject>Molecular and cellular biology</subject><subject>NADPH-Ferrihemoprotein Reductase - metabolism</subject><subject>Nucleotidases - metabolism</subject><subject>Succinate Dehydrogenase - metabolism</subject><subject>vaca</subject><subject>vache</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNpdkU1r3DAQhkVJSbdJf0KLQ0ppD071aY0OOYTQLwjkkOQsZHmc9WJZG2m3pf--cnbZQE8aMc_MvPMOIWeMXgjWwNdVly_uKOW8poLyzyC_AFNS1uwVWTDFVS2YgSOyOCBvyNucV-XLOFXH5Lhh0nBJF-TyHv1yGp62mKs-pmqdcO2S2wxxqmJftfH3MGEVXAgu_a1yiHGzrAKGNrkJ8yl53bsx47v9e0Ievn-7v_5Z39z--HV9dVN7qeWmBk4RW11iozi0UqD2ovMI1HAvuUGHTHnPdKNUx9BrXRLYctd1rQNoxQn5tOu7TnGWurFhyB7HsYiI22yBcdNopQt4_h-4its0FW2WgdZMAQdaKLOjfIo5J-ztOg3zgpZROxtsi8H22WA7u2dB2meDLSu17_cTtm3A7lC5d7TkP-7zLns39sUnP-QDBmCUpvCy0XJ4XP4ZEtoc3DiWpmwe3mhrbDmVKuCHHdi7aN1jKr0e7sp1G1qUGSHEPw0JmfU</recordid><startdate>198409</startdate><enddate>198409</enddate><creator>Pocius, P. 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A ; Dreels, J. M ; Devery-Pocius, J. E ; Baumrucker, C. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-820eeb74749528b43e7c3dce8092c429eae15cc17655d1ec77092eb2addba88b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>5'-Nucleotidase</topic><topic>Acid Phosphatase - metabolism</topic><topic>Animals</topic><topic>aparato golgi</topic><topic>appareil de golgi</topic><topic>Biological and medical sciences</topic><topic>Cattle - anatomy & histology</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Centrifugation, Zonal - methods</topic><topic>cows</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Female</topic><topic>fraccionamiento</topic><topic>fractionation</topic><topic>fractionnement</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galactosyltransferases - metabolism</topic><topic>gamma-Glutamyltransferase - metabolism</topic><topic>glande mammaire</topic><topic>glandulas mamarias</topic><topic>golgi apparatus</topic><topic>Golgi Apparatus - enzymology</topic><topic>Intracellular Membranes - ultrastructure</topic><topic>Lysosomes - enzymology</topic><topic>mammary glands</topic><topic>Mammary Glands, Animal - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Mitochondria - enzymology</topic><topic>Molecular and cellular biology</topic><topic>NADPH-Ferrihemoprotein Reductase - metabolism</topic><topic>Nucleotidases - metabolism</topic><topic>Succinate Dehydrogenase - metabolism</topic><topic>vaca</topic><topic>vache</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pocius, P. A</creatorcontrib><creatorcontrib>Dreels, J. M</creatorcontrib><creatorcontrib>Devery-Pocius, J. E</creatorcontrib><creatorcontrib>Baumrucker, C. R</creatorcontrib><creatorcontrib>Freie Univ. Berlin (Germany, F.R.). Fachbereich Veterinaermedizin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 50</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pocius, P. A</au><au>Dreels, J. M</au><au>Devery-Pocius, J. E</au><au>Baumrucker, C. R</au><aucorp>Freie Univ. Berlin (Germany, F.R.). Fachbereich Veterinaermedizin</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Techniques for preparation of bovine mammary smooth membranes</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>1984-09</date><risdate>1984</risdate><volume>67</volume><issue>9</issue><spage>2055</spage><epage>2061</epage><pages>2055-2061</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>Most procedures for fractionation of lactating mammary tissue membranes have utilized centrifuge tubes of small volume. We report a method for large-scale fractionation of bovine mammary membranes by a zonal rotor. Because of the large volume of the zonal rotor (1350 ml), greater amounts of starting material can be fractionated to obtain greater yields of desired fractions. We report results of linear sucrose gradients to fractionate mammary tissue homogenates and step gradient adaptations to accelerate membrane separations. Assay of membrane fractions for marker enzyme activities demonstrated that zonal fractionation and derived techniques were applicable for large scale preparation with higher yields of selected bovine mammary membranes. Application of this technique to small volumes is discussed for laboratories without a zonal rotor.</abstract><cop>Savoy, IL</cop><pub>Am Dairy Sci Assoc</pub><pmid>6149240</pmid><doi>10.3168/jds.S0022-0302(84)81544-1</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of dairy science, 1984-09, Vol.67 (9), p.2055-2061 |
| issn | 0022-0302 1525-3198 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Periodicals Index Online; Access via ScienceDirect (Elsevier) |
| subjects | 5'-Nucleotidase Acid Phosphatase - metabolism Animals aparato golgi appareil de golgi Biological and medical sciences Cattle - anatomy & histology Cell Membrane - enzymology Cell Membrane - ultrastructure Cell membranes. Ionic channels. Membrane pores Cell structures and functions Centrifugation, Zonal - methods cows Endoplasmic Reticulum - metabolism Female fraccionamiento fractionation fractionnement Fundamental and applied biological sciences. Psychology Galactosyltransferases - metabolism gamma-Glutamyltransferase - metabolism glande mammaire glandulas mamarias golgi apparatus Golgi Apparatus - enzymology Intracellular Membranes - ultrastructure Lysosomes - enzymology mammary glands Mammary Glands, Animal - ultrastructure Microscopy, Electron Mitochondria - enzymology Molecular and cellular biology NADPH-Ferrihemoprotein Reductase - metabolism Nucleotidases - metabolism Succinate Dehydrogenase - metabolism vaca vache |
| title | Techniques for preparation of bovine mammary smooth membranes |
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