GRF (somatocrinin) stimulates release of neurotensin, calcitonin and cAMP by a rat C cell line
We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5–180 min in serum-free defined medium. C...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-09, Vol.123 (2), p.497-506 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Zeytin, Füsun Brazeau, Paul |
| description | We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5–180 min in serum-free defined medium. CT, NT and cAMP are measured by RIA. At a maximally effective concentration of GRF (.1–1.0 nM), there is a 2–3 fold stimulation of CT release at 60–90 min with peak release at 180 min. In contrast, GRF causes a rapid 4–6 fold increase of NT release within 5–15 min. In 44–2 C cells there is a 4–40 fold stimulation of cAMP release by GRF. We conclude that in 44–2 C cells GRF stimulates release of NT and cAMP and show for the first time the effect of this peptide on release of CT. |
| doi_str_mv | 10.1016/0006-291X(84)90258-4 |
| format | Article |
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We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5–180 min in serum-free defined medium. CT, NT and cAMP are measured by RIA. At a maximally effective concentration of GRF (.1–1.0 nM), there is a 2–3 fold stimulation of CT release at 60–90 min with peak release at 180 min. In contrast, GRF causes a rapid 4–6 fold increase of NT release within 5–15 min. In 44–2 C cells there is a 4–40 fold stimulation of cAMP release by GRF. 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We conclude that in 44–2 C cells GRF stimulates release of NT and cAMP and show for the first time the effect of this peptide on release of CT.</description><subject>Animals</subject><subject>Calcitonin - metabolism</subject><subject>Cell Line</subject><subject>Clone Cells - metabolism</subject><subject>Cyclic AMP - metabolism</subject><subject>Growth Hormone-Releasing Hormone - pharmacology</subject><subject>Neurotensin - metabolism</subject><subject>Peptide Fragments - pharmacology</subject><subject>Rats</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFKHTEUhoO06NX6BgpZFYWOPZlkZpJNQS5qCxZLcdFVQyY5A5GZRJNMwbf3jvfi0q7O4v_Ofw4fIScMLhiw9isAtFWt2J8zKc4V1I2sxB5ZMVBQ1QzEB7J6Qw7IYc4PAIyJVu2T_RYUazmsyN-b39f0LMfJlGiTDz6c01z8NI-mYKYJRzQZaRxowDnFgiH78IVaM1pf4ganJjhqL3_-ov0zNTSZQtfU4jjS0Qf8RD4OZsx4vJtH5P766n79vbq9u_mxvrytLJeyVI0y0HQcVDu4QUkOUKtaDVyoBhsm67oXjeoa6dzQD6ZVTlronLO94Mqg4Efk87b2McWnGXPRk8_LEyZgnLOWrJadEu1_QcZVx3jXbECxBW2KOScc9GPyk0nPmoFe9OvFrV7cain0q369PHK665_7Cd3b0s73Jv-2zXEj45_HpLP1GCw6n9AW7aJ__8ALWeCS4Q</recordid><startdate>19840917</startdate><enddate>19840917</enddate><creator>Zeytin, Füsun</creator><creator>Brazeau, Paul</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SQ</scope><scope>7X8</scope></search><sort><creationdate>19840917</creationdate><title>GRF (somatocrinin) stimulates release of neurotensin, calcitonin and cAMP by a rat C cell line</title><author>Zeytin, Füsun ; Brazeau, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-59a0573096fdf983002929f3495e51822b459758ddfbfa69d8c07ddcb439ae43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Calcitonin - metabolism</topic><topic>Cell Line</topic><topic>Clone Cells - metabolism</topic><topic>Cyclic AMP - metabolism</topic><topic>Growth Hormone-Releasing Hormone - pharmacology</topic><topic>Neurotensin - metabolism</topic><topic>Peptide Fragments - pharmacology</topic><topic>Rats</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeytin, Füsun</creatorcontrib><creatorcontrib>Brazeau, Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Endocrinology Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeytin, Füsun</au><au>Brazeau, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GRF (somatocrinin) stimulates release of neurotensin, calcitonin and cAMP by a rat C cell line</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-09-17</date><risdate>1984</risdate><volume>123</volume><issue>2</issue><spage>497</spage><epage>506</epage><pages>497-506</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). 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| ispartof | Biochemical and biophysical research communications, 1984-09, Vol.123 (2), p.497-506 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Calcitonin - metabolism Cell Line Clone Cells - metabolism Cyclic AMP - metabolism Growth Hormone-Releasing Hormone - pharmacology Neurotensin - metabolism Peptide Fragments - pharmacology Rats Thyroid Neoplasms - metabolism Time Factors |
| title | GRF (somatocrinin) stimulates release of neurotensin, calcitonin and cAMP by a rat C cell line |
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