Dual promoter control of the escherichia coli lactose operon
The control of transcription initiation at the lactose operon promoter was investigated in vitro. We found that an upstream promoter (termed lac P 2) interfered with RNA polymerase binding at the principal promoter (termed lac P 1). The start site for lac P 2 was located at base pair position −22 re...
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| Veröffentlicht in: | Cell 1984-11, Vol.39 (1), p.173-180 |
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| creator | Malan, T.Philip McClure, William R. |
| description | The control of transcription initiation at the lactose operon promoter was investigated in vitro. We found that an upstream promoter (termed
lac P
2) interfered with RNA polymerase binding at the principal promoter (termed
lac P
1). The start site for
lac P
2 was located at base pair position −22 relative to the P
1 start site. The addition of cAMP receptor protein and cAMP was shown to repress
lac P
2 and to activate
lac P
1. Abortive initiation reactions for both promoters were used to investigate the coordinate repression-activation elicited by CRP-cAMP. The effects of
lac promoter mutations (L8, P
8, and UV5) were consistent with an important RNA polymerase positioning role for CRP-cAMP in the activation of
lac operon expression. |
| doi_str_mv | 10.1016/0092-8674(84)90203-4 |
| format | Article |
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lac P
2) interfered with RNA polymerase binding at the principal promoter (termed
lac P
1). The start site for
lac P
2 was located at base pair position −22 relative to the P
1 start site. The addition of cAMP receptor protein and cAMP was shown to repress
lac P
2 and to activate
lac P
1. Abortive initiation reactions for both promoters were used to investigate the coordinate repression-activation elicited by CRP-cAMP. The effects of
lac promoter mutations (L8, P
8, and UV5) were consistent with an important RNA polymerase positioning role for CRP-cAMP in the activation of
lac operon expression.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(84)90203-4</identifier><identifier>PMID: 6091909</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cyclic AMP - pharmacology ; Escherichia coli ; Escherichia coli - genetics ; Genes, Regulator ; Lac Operon - drug effects ; Promoter Regions, Genetic ; Receptors, Cyclic AMP - metabolism ; RNA, Messenger - genetics ; Transcription, Genetic</subject><ispartof>Cell, 1984-11, Vol.39 (1), p.173-180</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-23d8a4ae820549d65c0fbf8e1db377710c887fed1555ec53e2174a4c58e87dbd3</citedby><cites>FETCH-LOGICAL-c454t-23d8a4ae820549d65c0fbf8e1db377710c887fed1555ec53e2174a4c58e87dbd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(84)90203-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6091909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malan, T.Philip</creatorcontrib><creatorcontrib>McClure, William R.</creatorcontrib><title>Dual promoter control of the escherichia coli lactose operon</title><title>Cell</title><addtitle>Cell</addtitle><description>The control of transcription initiation at the lactose operon promoter was investigated in vitro. We found that an upstream promoter (termed
lac P
2) interfered with RNA polymerase binding at the principal promoter (termed
lac P
1). The start site for
lac P
2 was located at base pair position −22 relative to the P
1 start site. The addition of cAMP receptor protein and cAMP was shown to repress
lac P
2 and to activate
lac P
1. Abortive initiation reactions for both promoters were used to investigate the coordinate repression-activation elicited by CRP-cAMP. The effects of
lac promoter mutations (L8, P
8, and UV5) were consistent with an important RNA polymerase positioning role for CRP-cAMP in the activation of
lac operon expression.</description><subject>Cyclic AMP - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Genes, Regulator</subject><subject>Lac Operon - drug effects</subject><subject>Promoter Regions, Genetic</subject><subject>Receptors, Cyclic AMP - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMotVbfQGFWoovRk5lkkoAIUq9QcKPrkEnO0Mi0qcmM4Ns7taVLXZ3Ffzk_HyGnFK4o0OoaQBW5rAS7kOxSQQFlzvbImIISOaOi2CfjneWQHKX0AQCScz4iowoUVaDG5Oa-N222imEROoyZDcsuhjYLTdbNMcNk5xi9nXszSK3PWmO7kDALK4xheUwOGtMmPNneCXl_fHibPuez16eX6d0st4yzLi9KJw0zKAvgTLmKW2jqRiJ1dSmEoGClFA06OmxDy0ssqGCGWS5RCle7ckLON73Dzs8eU6cXPllsW7PE0CctaSGZEupfI2UlL0CIwcg2RhtDShEbvYp-YeK3pqDXdPUanV6j05LpX7qaDbGzbX9fL9DtQlucg3670XGg8eUx6mQ9Li06H9F22gX_94MfOP2IYQ</recordid><startdate>198411</startdate><enddate>198411</enddate><creator>Malan, T.Philip</creator><creator>McClure, William R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198411</creationdate><title>Dual promoter control of the escherichia coli lactose operon</title><author>Malan, T.Philip ; McClure, William R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-23d8a4ae820549d65c0fbf8e1db377710c887fed1555ec53e2174a4c58e87dbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Cyclic AMP - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Genes, Regulator</topic><topic>Lac Operon - drug effects</topic><topic>Promoter Regions, Genetic</topic><topic>Receptors, Cyclic AMP - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malan, T.Philip</creatorcontrib><creatorcontrib>McClure, William R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malan, T.Philip</au><au>McClure, William R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual promoter control of the escherichia coli lactose operon</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1984-11</date><risdate>1984</risdate><volume>39</volume><issue>1</issue><spage>173</spage><epage>180</epage><pages>173-180</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>The control of transcription initiation at the lactose operon promoter was investigated in vitro. We found that an upstream promoter (termed
lac P
2) interfered with RNA polymerase binding at the principal promoter (termed
lac P
1). The start site for
lac P
2 was located at base pair position −22 relative to the P
1 start site. The addition of cAMP receptor protein and cAMP was shown to repress
lac P
2 and to activate
lac P
1. Abortive initiation reactions for both promoters were used to investigate the coordinate repression-activation elicited by CRP-cAMP. The effects of
lac promoter mutations (L8, P
8, and UV5) were consistent with an important RNA polymerase positioning role for CRP-cAMP in the activation of
lac operon expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6091909</pmid><doi>10.1016/0092-8674(84)90203-4</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cell, 1984-11, Vol.39 (1), p.173-180 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81284979 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Cyclic AMP - pharmacology Escherichia coli Escherichia coli - genetics Genes, Regulator Lac Operon - drug effects Promoter Regions, Genetic Receptors, Cyclic AMP - metabolism RNA, Messenger - genetics Transcription, Genetic |
| title | Dual promoter control of the escherichia coli lactose operon |
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