Sensitive and Specific Morphine Radioimmunoassay with Iodine Label: Pharmacokinetics of Morphine in Man after Intravenous Administration
Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses co...
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| Veröffentlicht in: | Annals of Clinical Biochemistry 1984-07, Vol.21 (4), p.318-325 |
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| container_end_page | 325 |
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| container_issue | 4 |
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| container_title | Annals of Clinical Biochemistry |
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| creator | Moore, R A Baldwin, D Allen, M C Watson, P J Q Bullingham, R E S McQuay, H J |
| description | Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses commercially available anti-morphine antiserum and separating reagent with a simply prepared iodinated morphine derivative. Cross-reactivity to morphine metabolites is negligible and the method correlates well with HPLC. Results obtained for pharmacokinetic parameters after intravenous administration in man are in agreement with published data using GLC or HPLC. The method is inexpensive, simple and rapid; choice of a different antiserum could allow the method to be used for screening for drugs of abuse such as morphine, diamorphine, codeine and dihydrocodeine. |
| doi_str_mv | 10.1177/000456328402100415 |
| format | Article |
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This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses commercially available anti-morphine antiserum and separating reagent with a simply prepared iodinated morphine derivative. Cross-reactivity to morphine metabolites is negligible and the method correlates well with HPLC. Results obtained for pharmacokinetic parameters after intravenous administration in man are in agreement with published data using GLC or HPLC. The method is inexpensive, simple and rapid; choice of a different antiserum could allow the method to be used for screening for drugs of abuse such as morphine, diamorphine, codeine and dihydrocodeine.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/000456328402100415</identifier><identifier>PMID: 6486707</identifier><identifier>CODEN: ACBOBU</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Cross Reactions ; General pharmacology ; Humans ; Injections, Intravenous ; Iodine Radioisotopes ; Kinetics ; Laminectomy ; Medical sciences ; Middle Aged ; Morphine - administration & dosage ; Morphine - blood ; Pharmacology. Drug treatments ; Radioimmunoassay - methods ; Time Factors</subject><ispartof>Annals of Clinical Biochemistry, 1984-07, Vol.21 (4), p.318-325</ispartof><rights>1984 Association for Clinical Biochemistry</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-530b875d112b77b7226658d469e6aba904ad21dd3a391a834a8ccae6b1c5399e3</citedby><cites>FETCH-LOGICAL-c398t-530b875d112b77b7226658d469e6aba904ad21dd3a391a834a8ccae6b1c5399e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>313,314,776,780,788,27899,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8965031$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6486707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moore, R A</creatorcontrib><creatorcontrib>Baldwin, D</creatorcontrib><creatorcontrib>Allen, M C</creatorcontrib><creatorcontrib>Watson, P J Q</creatorcontrib><creatorcontrib>Bullingham, R E S</creatorcontrib><creatorcontrib>McQuay, H J</creatorcontrib><title>Sensitive and Specific Morphine Radioimmunoassay with Iodine Label: Pharmacokinetics of Morphine in Man after Intravenous Administration</title><title>Annals of Clinical Biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses commercially available anti-morphine antiserum and separating reagent with a simply prepared iodinated morphine derivative. Cross-reactivity to morphine metabolites is negligible and the method correlates well with HPLC. Results obtained for pharmacokinetic parameters after intravenous administration in man are in agreement with published data using GLC or HPLC. The method is inexpensive, simple and rapid; choice of a different antiserum could allow the method to be used for screening for drugs of abuse such as morphine, diamorphine, codeine and dihydrocodeine.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cross Reactions</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Injections, Intravenous</subject><subject>Iodine Radioisotopes</subject><subject>Kinetics</subject><subject>Laminectomy</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Morphine - administration & dosage</subject><subject>Morphine - blood</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Radioimmunoassay - methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moore, R A</creatorcontrib><creatorcontrib>Baldwin, D</creatorcontrib><creatorcontrib>Allen, M C</creatorcontrib><creatorcontrib>Watson, P J Q</creatorcontrib><creatorcontrib>Bullingham, R E S</creatorcontrib><creatorcontrib>McQuay, H J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of Clinical Biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moore, R A</au><au>Baldwin, D</au><au>Allen, M C</au><au>Watson, P J Q</au><au>Bullingham, R E S</au><au>McQuay, H J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive and Specific Morphine Radioimmunoassay with Iodine Label: Pharmacokinetics of Morphine in Man after Intravenous Administration</atitle><jtitle>Annals of Clinical Biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>1984-07</date><risdate>1984</risdate><volume>21</volume><issue>4</issue><spage>318</spage><epage>325</epage><pages>318-325</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><coden>ACBOBU</coden><abstract>Analysis of morphine in plasma by radioimmunoassay is complicated by interference from morphine metabolites, particularly morphine-3-glucuronide. This interference makes radioimmunoassay a poor technique to study the pharmacokinetics of morphine in man. The method described here is one which uses commercially available anti-morphine antiserum and separating reagent with a simply prepared iodinated morphine derivative. Cross-reactivity to morphine metabolites is negligible and the method correlates well with HPLC. Results obtained for pharmacokinetic parameters after intravenous administration in man are in agreement with published data using GLC or HPLC. The method is inexpensive, simple and rapid; choice of a different antiserum could allow the method to be used for screening for drugs of abuse such as morphine, diamorphine, codeine and dihydrocodeine.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>6486707</pmid><doi>10.1177/000456328402100415</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-5632 |
| ispartof | Annals of Clinical Biochemistry, 1984-07, Vol.21 (4), p.318-325 |
| issn | 0004-5632 1758-1001 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analysis Biological and medical sciences Chromatography, High Pressure Liquid Cross Reactions General pharmacology Humans Injections, Intravenous Iodine Radioisotopes Kinetics Laminectomy Medical sciences Middle Aged Morphine - administration & dosage Morphine - blood Pharmacology. Drug treatments Radioimmunoassay - methods Time Factors |
| title | Sensitive and Specific Morphine Radioimmunoassay with Iodine Label: Pharmacokinetics of Morphine in Man after Intravenous Administration |
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