Effect of 2,3-diphosphoglycerate on the Bohr effect of human adult hemoglobin
The effect of 2,3-diphosphoglycerate (DPG) on the Bohr effect of human hemoglobin has been studied by means of hydrogen ion titration techniques. The results indicate a) that both the acid and the alkaline Bohr effect are equally affected, b) that the DPG binding to deoxyhemoglobin (Hb) is much stro...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1971-10, Vol.45 (2), p.544-550 |
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| container_title | Biochemical and biophysical research communications |
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| creator | De Bruin, S.H. Janssen, L.H.M. Van Os, G.A.J. |
| description | The effect of 2,3-diphosphoglycerate (DPG) on the Bohr effect of human hemoglobin has been studied by means of hydrogen ion titration techniques. The results indicate a) that both the acid and the alkaline Bohr effect are equally affected, b) that the DPG binding to deoxyhemoglobin (Hb) is much stronger than to carboxyhemoglobin (HbCO) and c) that Hb binds effectively one DPG molecule. The effect on the Bohr effect can roughly be described by assuming that upon binding two groups per tetramer change their pK from 6.8 to 7.8 and two others from 6.8 to 5.8. These groups very probably are the imidazole groups of the two histidines H21 (143)β and the two phosphate groups of DPG (second dissociation). From the experiments a value for the dissociation constant K of the Hb-DPG complex of about 10
−5 M
−1 could be estimated at pH 6.2 and pH 7.5. |
| doi_str_mv | 10.1016/0006-291X(71)90854-0 |
| format | Article |
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−5 M
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−5 M
−1 could be estimated at pH 6.2 and pH 7.5.</description><subject>Adult</subject><subject>Carbon Monoxide</subject><subject>Chromatography, Ion Exchange</subject><subject>Glycerophosphates</subject><subject>Hemoglobins</subject><subject>Histidine</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Imidazoles</subject><subject>Oxygen</subject><subject>Phosphates</subject><subject>Protein Binding</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo67r6DxR6EgWrk6RJuxdBl_UDVrwoeAttMrGVtlmTVth_b9dd9uhhmMM87wzzEHJK4ZoClTcAIGM2pR8XKb2cQiaSGPbImMIUYkYh2SfjHXJIjkL4AqA0kdMRGQkqIeV0TF7m1qLuImcjdsVjUy1LF4b6rFcafd5h5NqoKzG6d6WPcAeXfZO3UW76uotKbAbeFVV7TA5sXgc82fYJeX-Yv82e4sXr4_PsbhHrBHgXJykruDUiZ6BZKpGnCae6AC5AZjaxIFFKbSRiWiAtRCqZsCI3xgJwo5FPyPlm79K77x5Dp5oqaKzrvEXXB5VRJjKZsQFMNqD2LgSPVi191eR-pSiotUW1VqTWilRK1Z9FBUPsbLu_Lxo0u9BW2zC_3cxxePKnQq-CrrDVaCo_CFLGVf8f-AV7VICo</recordid><startdate>19711015</startdate><enddate>19711015</enddate><creator>De Bruin, S.H.</creator><creator>Janssen, L.H.M.</creator><creator>Van Os, G.A.J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19711015</creationdate><title>Effect of 2,3-diphosphoglycerate on the Bohr effect of human adult hemoglobin</title><author>De Bruin, S.H. ; Janssen, L.H.M. ; Van Os, G.A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-472b3fd5a20c276e37431cb035068f4f06e66cd6ee7be1b57625f5addf003dce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Adult</topic><topic>Carbon Monoxide</topic><topic>Chromatography, Ion Exchange</topic><topic>Glycerophosphates</topic><topic>Hemoglobins</topic><topic>Histidine</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Imidazoles</topic><topic>Oxygen</topic><topic>Phosphates</topic><topic>Protein Binding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Bruin, S.H.</creatorcontrib><creatorcontrib>Janssen, L.H.M.</creatorcontrib><creatorcontrib>Van Os, G.A.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Bruin, S.H.</au><au>Janssen, L.H.M.</au><au>Van Os, G.A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 2,3-diphosphoglycerate on the Bohr effect of human adult hemoglobin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1971-10-15</date><risdate>1971</risdate><volume>45</volume><issue>2</issue><spage>544</spage><epage>550</epage><pages>544-550</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The effect of 2,3-diphosphoglycerate (DPG) on the Bohr effect of human hemoglobin has been studied by means of hydrogen ion titration techniques. The results indicate a) that both the acid and the alkaline Bohr effect are equally affected, b) that the DPG binding to deoxyhemoglobin (Hb) is much stronger than to carboxyhemoglobin (HbCO) and c) that Hb binds effectively one DPG molecule. The effect on the Bohr effect can roughly be described by assuming that upon binding two groups per tetramer change their pK from 6.8 to 7.8 and two others from 6.8 to 5.8. These groups very probably are the imidazole groups of the two histidines H21 (143)β and the two phosphate groups of DPG (second dissociation). From the experiments a value for the dissociation constant K of the Hb-DPG complex of about 10
−5 M
−1 could be estimated at pH 6.2 and pH 7.5.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5160731</pmid><doi>10.1016/0006-291X(71)90854-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1971-10, Vol.45 (2), p.544-550 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Carbon Monoxide Chromatography, Ion Exchange Glycerophosphates Hemoglobins Histidine Humans Hydrogen-Ion Concentration Imidazoles Oxygen Phosphates Protein Binding |
| title | Effect of 2,3-diphosphoglycerate on the Bohr effect of human adult hemoglobin |
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