The Effect of Tetranitromethane on Apo‐Aspartate‐Aminotransferase from Pig Heart
Both holo‐ and apo‐aspartate‐aminotransferase are sensitive to tetranitromethane; however, the inactivation which follows nitration is partial for the holoenzyme, and complete for the apoenzyme. Cysteinyl and tyrosyl residues are modified in both forms of the enzyme; in the apoenzyme a greater numbe...
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| Veröffentlicht in: | European journal of biochemistry 1971-11, Vol.23 (2), p.349-354 |
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| container_end_page | 354 |
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| container_title | European journal of biochemistry |
| container_volume | 23 |
| creator | Barra, Donatella Bossa, Francesco Ferraro, Anna Giartosio, Anna Turano, Carlo |
| description | Both holo‐ and apo‐aspartate‐aminotransferase are sensitive to tetranitromethane; however, the inactivation which follows nitration is partial for the holoenzyme, and complete for the apoenzyme. Cysteinyl and tyrosyl residues are modified in both forms of the enzyme; in the apoenzyme a greater number of tyrosines are available to the reagent. The complete inactivation of the apoenzyme is correlated with the loss of only one tyrosyl residue, which is selectively modified under particular conditions. The loss of activity is caused by the failure of the nitrated apoenzyme to recombine with the coenzyme. |
| doi_str_mv | 10.1111/j.1432-1033.1971.tb01628.x |
| format | Article |
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Cysteinyl and tyrosyl residues are modified in both forms of the enzyme; in the apoenzyme a greater number of tyrosines are available to the reagent. The complete inactivation of the apoenzyme is correlated with the loss of only one tyrosyl residue, which is selectively modified under particular conditions. 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Cysteinyl and tyrosyl residues are modified in both forms of the enzyme; in the apoenzyme a greater number of tyrosines are available to the reagent. The complete inactivation of the apoenzyme is correlated with the loss of only one tyrosyl residue, which is selectively modified under particular conditions. The loss of activity is caused by the failure of the nitrated apoenzyme to recombine with the coenzyme.</description><subject>Animals</subject><subject>Aspartate Aminotransferases - antagonists & inhibitors</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Cysteine</subject><subject>Methane</subject><subject>Myocardium - enzymology</subject><subject>Nitro Compounds</subject><subject>Swine</subject><subject>Tyrosine</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMFKw0AQhhdRaq0-ghA8eGvcyWZ3Ey9SS2uFgoL1vGSTWZvSNHE3xfbmI_iMPokJDb07lxn4_39m-Ai5AepDU3crH0IWDIEy5kMswa81BRFE_u6E9I_SKelTCuEwiLk4JxfOrSilIhayR3ochIwY7ZPFYonexBhMa6803gJrm2zy2pYF1stkg1658UZV-fv9M3JVYuukxnYu8k3ZOp1Bmzj0TBPwXvMPb4aN6ZKcmWTt8KrrA_I-nSzGs-H85el5PJoPUyZp3DwJsZYp47EOUgEMUmEkaipkwDlkWmgMWJZoFsrIcJNxnoYxUIOaQxSJkA3I7WFvZcvPLbpaFblLcb1uHi-3TkUQcBYK2RjvD8bUls5ZNKqyeZHYvQKqWqRqpVpuquWmWqSqQ6p2Tfi6u7LVBWbHaMew0R8O-le-xv0_Nqvp5PGNhTH7A5GniCs</recordid><startdate>19711111</startdate><enddate>19711111</enddate><creator>Barra, Donatella</creator><creator>Bossa, Francesco</creator><creator>Ferraro, Anna</creator><creator>Giartosio, Anna</creator><creator>Turano, Carlo</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19711111</creationdate><title>The Effect of Tetranitromethane on Apo‐Aspartate‐Aminotransferase from Pig Heart</title><author>Barra, Donatella ; Bossa, Francesco ; Ferraro, Anna ; Giartosio, Anna ; Turano, Carlo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3709-1019b7c359b2c6131c6f7eb0672551db6be23dab3478f5fd55c4910feb5188643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Animals</topic><topic>Aspartate Aminotransferases - antagonists & inhibitors</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Cysteine</topic><topic>Methane</topic><topic>Myocardium - enzymology</topic><topic>Nitro Compounds</topic><topic>Swine</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barra, Donatella</creatorcontrib><creatorcontrib>Bossa, Francesco</creatorcontrib><creatorcontrib>Ferraro, Anna</creatorcontrib><creatorcontrib>Giartosio, Anna</creatorcontrib><creatorcontrib>Turano, Carlo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barra, Donatella</au><au>Bossa, Francesco</au><au>Ferraro, Anna</au><au>Giartosio, Anna</au><au>Turano, Carlo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Tetranitromethane on Apo‐Aspartate‐Aminotransferase from Pig Heart</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1971-11-11</date><risdate>1971</risdate><volume>23</volume><issue>2</issue><spage>349</spage><epage>354</epage><pages>349-354</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>Both holo‐ and apo‐aspartate‐aminotransferase are sensitive to tetranitromethane; however, the inactivation which follows nitration is partial for the holoenzyme, and complete for the apoenzyme. 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| ispartof | European journal of biochemistry, 1971-11, Vol.23 (2), p.349-354 |
| issn | 0014-2956 1432-1033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81253467 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Aspartate Aminotransferases - antagonists & inhibitors Chemical Phenomena Chemistry Cysteine Methane Myocardium - enzymology Nitro Compounds Swine Tyrosine |
| title | The Effect of Tetranitromethane on Apo‐Aspartate‐Aminotransferase from Pig Heart |
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