A buoyant method for the determination of the superhelix density of closed circular DNA

A convenient method for the determination of the superhelix density of a closed circular DNA has been developed and tested with a series of closed DNA's with different superhelix densities and molecular weights. The method is based on the superhelix density-dependent binding affinity of closed...

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Veröffentlicht in:	Journal of molecular biology 1971-11, Vol.62 (1), p.1-19
Hauptverfasser:	Gray, Horace B., Upholt, William B., Vinograd, Jerome
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Centrifugation, Density Gradient DNA, Viral Molecular Biology Molecular Weight Nucleic Acid Conformation Quaternary Ammonium Compounds Quinolines Simian virus 40 Spectrophotometry Ultracentrifugation
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