The effect of high hydrostatic pressure and low temperature on lactic dehydrogenase and glutamic oxalacetic transaminase
LDH and GOT can be used with assurance as indicators of pressure-temperature effects in most regions of interest, specifically below 20,000 psi. LDH was susceptible to pressure deactivation at pressure levels below those tolerated by chymotrypsin, trypsin and alpha-amylase of Bacillus subtilis (17,...
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| Veröffentlicht in: | Cryobiology 1971-12, Vol.8 (6), p.524-534 |
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| container_end_page | 534 |
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| container_issue | 6 |
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| container_title | Cryobiology |
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| creator | Carter, J.E. Graham, E.F. Lillehei, R.C. Blackshear, P.L. |
| description | LDH and GOT can be used with assurance as indicators of pressure-temperature effects in most regions of interest, specifically below 20,000 psi. LDH was susceptible to pressure deactivation at pressure levels below those tolerated by chymotrypsin, trypsin and alpha-amylase of
Bacillus subtilis (17, 18, 20). Samples of LDH and GOT cooled to −20 °C were deactivated to the greatest extent by the application of pressure. The presence of glycerine and DMSO appeared to increase the sensitivity of GOT and LDH to pressure deactivation. When pressure was applied before cooling all pressures above 15,000 psi resulted in some deactivation of LDH and all pressures above 20,000 psi resulted in some deactivation of GOT. |
| doi_str_mv | 10.1016/0011-2240(71)90003-4 |
| format | Article |
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Bacillus subtilis (17, 18, 20). Samples of LDH and GOT cooled to −20 °C were deactivated to the greatest extent by the application of pressure. The presence of glycerine and DMSO appeared to increase the sensitivity of GOT and LDH to pressure deactivation. When pressure was applied before cooling all pressures above 15,000 psi resulted in some deactivation of LDH and all pressures above 20,000 psi resulted in some deactivation of GOT.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/0011-2240(71)90003-4</identifier><identifier>PMID: 5137285</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Aspartate Aminotransferases ; Cold Temperature ; Dogs ; In Vitro Techniques ; Kidney - enzymology ; L-Lactate Dehydrogenase ; Perfusion ; Pressure ; Protein Denaturation ; Solutions ; Tissue Preservation</subject><ispartof>Cryobiology, 1971-12, Vol.8 (6), p.524-534</ispartof><rights>1972</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-5169a9d9089051194c1cdcf3218b6c90a3653648893f1c0f2c1dbb7c835478b83</citedby><cites>FETCH-LOGICAL-c357t-5169a9d9089051194c1cdcf3218b6c90a3653648893f1c0f2c1dbb7c835478b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0011-2240(71)90003-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5137285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carter, J.E.</creatorcontrib><creatorcontrib>Graham, E.F.</creatorcontrib><creatorcontrib>Lillehei, R.C.</creatorcontrib><creatorcontrib>Blackshear, P.L.</creatorcontrib><title>The effect of high hydrostatic pressure and low temperature on lactic dehydrogenase and glutamic oxalacetic transaminase</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>LDH and GOT can be used with assurance as indicators of pressure-temperature effects in most regions of interest, specifically below 20,000 psi. LDH was susceptible to pressure deactivation at pressure levels below those tolerated by chymotrypsin, trypsin and alpha-amylase of
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Bacillus subtilis (17, 18, 20). Samples of LDH and GOT cooled to −20 °C were deactivated to the greatest extent by the application of pressure. The presence of glycerine and DMSO appeared to increase the sensitivity of GOT and LDH to pressure deactivation. When pressure was applied before cooling all pressures above 15,000 psi resulted in some deactivation of LDH and all pressures above 20,000 psi resulted in some deactivation of GOT.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>5137285</pmid><doi>10.1016/0011-2240(71)90003-4</doi><tpages>11</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Aspartate Aminotransferases Cold Temperature Dogs In Vitro Techniques Kidney - enzymology L-Lactate Dehydrogenase Perfusion Pressure Protein Denaturation Solutions Tissue Preservation |
| title | The effect of high hydrostatic pressure and low temperature on lactic dehydrogenase and glutamic oxalacetic transaminase |
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