Radioimmunoassay of cationic trypsin-like immunoreactivity in man
The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit ca...
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| Veröffentlicht in: | Scandinavian journal of gastroenterology 1984-07, Vol.19 (5), p.613-621 |
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| container_end_page | 621 |
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| container_issue | 5 |
| container_start_page | 613 |
| container_title | Scandinavian journal of gastroenterology |
| container_volume | 19 |
| creator | FLORHOLMEN, J JORDE, R OLSEN, R. L KOLMANNSKOG, S BURHOL, P. G |
| description | The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum. |
| doi_str_mv | 10.1080/00365521.1984.12005780 |
| format | Article |
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G</creatorcontrib><description>The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.</description><identifier>ISSN: 0036-5521</identifier><identifier>EISSN: 1502-7708</identifier><identifier>DOI: 10.1080/00365521.1984.12005780</identifier><identifier>PMID: 6474083</identifier><identifier>CODEN: SJGRA4</identifier><language>eng</language><publisher>Copenhagen: Scandinavian University Press</publisher><subject>Biological and medical sciences ; Duodenum - analysis ; Electrophoresis, Polyacrylamide Gel ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunoelectrophoresis ; Intestinal Secretions - analysis ; Iodine Radioisotopes ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Medical sciences ; Other diseases. 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G</creatorcontrib><title>Radioimmunoassay of cationic trypsin-like immunoreactivity in man</title><title>Scandinavian journal of gastroenterology</title><addtitle>Scand J Gastroenterol</addtitle><description>The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.</description><subject>Biological and medical sciences</subject><subject>Duodenum - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunoelectrophoresis</subject><subject>Intestinal Secretions - analysis</subject><subject>Iodine Radioisotopes</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Medical sciences</subject><subject>Other diseases. Semiology</subject><subject>Radioimmunoassay - methods</subject><subject>Tosyllysine Chloromethyl Ketone</subject><subject>Trypsin - immunology</subject><subject>Trypsin - metabolism</subject><issn>0036-5521</issn><issn>1502-7708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo67r6E5QexFvXSZrP47L4BQuC7D2kSQrRfqxNK_Tf27Jdr15mGN5nZuBB6A7DGoOER4CMM0bwGitJ15gAMCHhDC0xA5IKAfIcLSconahLdBXjJ0wQVQu04FRQkNkSbT6MC02oqr5uTIxmSJoisaYLTR1s0rXDIYY6LcOXT45Q643twk_ohiTUSWXqa3RRmDL6m7mv0P75ab99TXfvL2_bzS61Geddqhyw3AslPHBJBSvcOBnw1jLMDVY5d2osVDqPnaOMEkpdLoylBDKRZyv0cDx7aJvv3sdOVyFaX5am9k0ftcSEcMnVvyDOFGVYiRHkR9C2TYytL_ShDZVpB41BT471ybGeHOuT43Hxdv7Q55V3f2uz1DG_n3MTrSmL1tQ2xD9MqowQSbJf9ImECA</recordid><startdate>198407</startdate><enddate>198407</enddate><creator>FLORHOLMEN, J</creator><creator>JORDE, R</creator><creator>OLSEN, R. 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Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunoelectrophoresis</topic><topic>Intestinal Secretions - analysis</topic><topic>Iodine Radioisotopes</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Medical sciences</topic><topic>Other diseases. Semiology</topic><topic>Radioimmunoassay - methods</topic><topic>Tosyllysine Chloromethyl Ketone</topic><topic>Trypsin - immunology</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FLORHOLMEN, J</creatorcontrib><creatorcontrib>JORDE, R</creatorcontrib><creatorcontrib>OLSEN, R. L</creatorcontrib><creatorcontrib>KOLMANNSKOG, S</creatorcontrib><creatorcontrib>BURHOL, P. 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G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay of cationic trypsin-like immunoreactivity in man</atitle><jtitle>Scandinavian journal of gastroenterology</jtitle><addtitle>Scand J Gastroenterol</addtitle><date>1984-07</date><risdate>1984</risdate><volume>19</volume><issue>5</issue><spage>613</spage><epage>621</epage><pages>613-621</pages><issn>0036-5521</issn><eissn>1502-7708</eissn><coden>SJGRA4</coden><abstract>The present paper describes the preparation of human cationic trypsin and of a stable and fully immunoreactive 125I-labelled tosyl-lysine chloromethyl ketone (TLCK) cationic trypsin by a modified chloramine-T method with a high specific radioactivity; the production of an avid and specific rabbit cationic trypsin antiserum; and a sensitive, precise, and specific radioimmunoassay method enabling measurements of fasting serum trypsin-like immunoreactivity (CTLI) in the low ng/ml range in normals; the significant rise in serum CTLI in patients with normal pancreatic exocrine secretion; and the absence of any rise in patients with severely reduced pancreatic exocrine secretion after intravenous injection of secretion; a markedly elevated fasting serum CTLI level in patients with acute pancreatitis; fasting CTLI in duodenal juice; and separation of CTLI in one minor and one major molecular component in fasting serum.</abstract><cop>Copenhagen</cop><cop>Oslo</cop><cop>Stockholm</cop><pub>Scandinavian University Press</pub><pmid>6474083</pmid><doi>10.1080/00365521.1984.12005780</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-5521 |
| ispartof | Scandinavian journal of gastroenterology, 1984-07, Vol.19 (5), p.613-621 |
| issn | 0036-5521 1502-7708 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81226869 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Biological and medical sciences Duodenum - analysis Electrophoresis, Polyacrylamide Gel Gastroenterology. Liver. Pancreas. Abdomen Humans Immunoelectrophoresis Intestinal Secretions - analysis Iodine Radioisotopes Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Other diseases. Semiology Radioimmunoassay - methods Tosyllysine Chloromethyl Ketone Trypsin - immunology Trypsin - metabolism |
| title | Radioimmunoassay of cationic trypsin-like immunoreactivity in man |
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