Induction of fruiting in two aggregateless mutants of Dictyostelium discoideum
Five general groups of morphogenetically aberrant mutants of Dictyostelium discoideum were isolated. Each group of mutants was characterized either by the absence of any fruiting structures or by the formation of abnormal fructifications. Among these developmental mutants were two aggregateless isol...
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| Veröffentlicht in: | Developmental biology 1971-12, Vol.26 (4), p.606-615 |
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| container_title | Developmental biology |
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| creator | Weber, Allen T. Raper, Kenneth B. |
| description | Five general groups of morphogenetically aberrant mutants of
Dictyostelium discoideum were isolated. Each group of mutants was characterized either by the absence of any fruiting structures or by the formation of abnormal fructifications. Among these developmental mutants were two aggregateless isolates, Agg
−-1 and Agg
−-2, that could be induced to form normal sorocarps under certain conditions. Sorocarps of the normal
D. discoideum type were formed when growing myxamoebae from either of these mutants were allowed to come in contact with myxamoebae of the other mutants, wild-type
D. discoideum, D. purpureum, or
D. mucoroides. No sorocarps were formed when myxamoebae of Agg
−-1 and Agg
−-2 were paired. These two aggregateless mutants, while incapable of aggregating or fruiting when cultivated singly with
Escherichia coli B/r on a glucose-salts medium, formed normal fruiting structures after being freed of what appeared to be a product of bacterial growth. The spores produced by Agg
−-1 and Agg
−-2 myxamoebae again gave rise to aggregateless clones of the original parental types. |
| doi_str_mv | 10.1016/0012-1606(71)90143-6 |
| format | Article |
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Dictyostelium discoideum were isolated. Each group of mutants was characterized either by the absence of any fruiting structures or by the formation of abnormal fructifications. Among these developmental mutants were two aggregateless isolates, Agg
−-1 and Agg
−-2, that could be induced to form normal sorocarps under certain conditions. Sorocarps of the normal
D. discoideum type were formed when growing myxamoebae from either of these mutants were allowed to come in contact with myxamoebae of the other mutants, wild-type
D. discoideum, D. purpureum, or
D. mucoroides. No sorocarps were formed when myxamoebae of Agg
−-1 and Agg
−-2 were paired. These two aggregateless mutants, while incapable of aggregating or fruiting when cultivated singly with
Escherichia coli B/r on a glucose-salts medium, formed normal fruiting structures after being freed of what appeared to be a product of bacterial growth. The spores produced by Agg
−-1 and Agg
−-2 myxamoebae again gave rise to aggregateless clones of the original parental types.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(71)90143-6</identifier><identifier>PMID: 4944076</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Aggregation ; Contact Inhibition ; Culture Media ; Escherichia coli ; Mutation ; Myxomycetes - growth & development ; Myxomycetes - isolation & purification ; Nitrosoguanidines ; Reproduction ; Spores - growth & development ; Spores, Fungal ; Ultraviolet Rays</subject><ispartof>Developmental biology, 1971-12, Vol.26 (4), p.606-615</ispartof><rights>1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-73f0b81267d11addf8c6cf3e55b385fc07a1eb96b498736f7cf38591c6984edd3</citedby><cites>FETCH-LOGICAL-c272t-73f0b81267d11addf8c6cf3e55b385fc07a1eb96b498736f7cf38591c6984edd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(71)90143-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4944076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, Allen T.</creatorcontrib><creatorcontrib>Raper, Kenneth B.</creatorcontrib><title>Induction of fruiting in two aggregateless mutants of Dictyostelium discoideum</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Five general groups of morphogenetically aberrant mutants of
Dictyostelium discoideum were isolated. Each group of mutants was characterized either by the absence of any fruiting structures or by the formation of abnormal fructifications. Among these developmental mutants were two aggregateless isolates, Agg
−-1 and Agg
−-2, that could be induced to form normal sorocarps under certain conditions. Sorocarps of the normal
D. discoideum type were formed when growing myxamoebae from either of these mutants were allowed to come in contact with myxamoebae of the other mutants, wild-type
D. discoideum, D. purpureum, or
D. mucoroides. No sorocarps were formed when myxamoebae of Agg
−-1 and Agg
−-2 were paired. These two aggregateless mutants, while incapable of aggregating or fruiting when cultivated singly with
Escherichia coli B/r on a glucose-salts medium, formed normal fruiting structures after being freed of what appeared to be a product of bacterial growth. The spores produced by Agg
−-1 and Agg
−-2 myxamoebae again gave rise to aggregateless clones of the original parental types.</description><subject>Cell Aggregation</subject><subject>Contact Inhibition</subject><subject>Culture Media</subject><subject>Escherichia coli</subject><subject>Mutation</subject><subject>Myxomycetes - growth & development</subject><subject>Myxomycetes - isolation & purification</subject><subject>Nitrosoguanidines</subject><subject>Reproduction</subject><subject>Spores - growth & development</subject><subject>Spores, Fungal</subject><subject>Ultraviolet Rays</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAQxy0EKuXxDUDKhGAI-BrHThYkVF6VKlhAYrMS-1wZNXGxHVC_PQmtOjLd8H_c3Y-QM6DXQIHfUAqTFDjllwKuSgosS_keGQMt8zTn7GOfjHeWQ3IUwielNCuKbERGrGSMCj4mL7NWdypa1ybOJMZ3Ntp2kdg2iT8uqRYLj4sq4hJDSJouVm0Mg_Heqrh2oRds1yTaBuWsxq45IQemWgY83c5j8v748DZ9TuevT7Pp3TxVEzGJqcgMrQuYcKEBKq1NobgyGeZ5nRW5UVRUgHXJa1YWIuNG9GKRl6B4WTDUOjsmF5velXdfHYYom_4GXC6rFl0XZN8NDCjrjWxjVN6F4NHIlbdN5dcSqBwwyoGRHBhJAfIPo-R97Hzb39UN6l1oy63Xbzc69k9-W_QyKIutQm09qii1s_8v-AU_QYJS</recordid><startdate>197112</startdate><enddate>197112</enddate><creator>Weber, Allen T.</creator><creator>Raper, Kenneth B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197112</creationdate><title>Induction of fruiting in two aggregateless mutants of Dictyostelium discoideum</title><author>Weber, Allen T. ; Raper, Kenneth B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-73f0b81267d11addf8c6cf3e55b385fc07a1eb96b498736f7cf38591c6984edd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Cell Aggregation</topic><topic>Contact Inhibition</topic><topic>Culture Media</topic><topic>Escherichia coli</topic><topic>Mutation</topic><topic>Myxomycetes - growth & development</topic><topic>Myxomycetes - isolation & purification</topic><topic>Nitrosoguanidines</topic><topic>Reproduction</topic><topic>Spores - growth & development</topic><topic>Spores, Fungal</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weber, Allen T.</creatorcontrib><creatorcontrib>Raper, Kenneth B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, Allen T.</au><au>Raper, Kenneth B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of fruiting in two aggregateless mutants of Dictyostelium discoideum</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1971-12</date><risdate>1971</risdate><volume>26</volume><issue>4</issue><spage>606</spage><epage>615</epage><pages>606-615</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Five general groups of morphogenetically aberrant mutants of
Dictyostelium discoideum were isolated. Each group of mutants was characterized either by the absence of any fruiting structures or by the formation of abnormal fructifications. Among these developmental mutants were two aggregateless isolates, Agg
−-1 and Agg
−-2, that could be induced to form normal sorocarps under certain conditions. Sorocarps of the normal
D. discoideum type were formed when growing myxamoebae from either of these mutants were allowed to come in contact with myxamoebae of the other mutants, wild-type
D. discoideum, D. purpureum, or
D. mucoroides. No sorocarps were formed when myxamoebae of Agg
−-1 and Agg
−-2 were paired. These two aggregateless mutants, while incapable of aggregating or fruiting when cultivated singly with
Escherichia coli B/r on a glucose-salts medium, formed normal fruiting structures after being freed of what appeared to be a product of bacterial growth. The spores produced by Agg
−-1 and Agg
−-2 myxamoebae again gave rise to aggregateless clones of the original parental types.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4944076</pmid><doi>10.1016/0012-1606(71)90143-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Developmental biology, 1971-12, Vol.26 (4), p.606-615 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81214104 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cell Aggregation Contact Inhibition Culture Media Escherichia coli Mutation Myxomycetes - growth & development Myxomycetes - isolation & purification Nitrosoguanidines Reproduction Spores - growth & development Spores, Fungal Ultraviolet Rays |
| title | Induction of fruiting in two aggregateless mutants of Dictyostelium discoideum |
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