A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes
A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27−; 2) six families,...
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| Veröffentlicht in: | Human immunology 1984-08, Vol.10 (4), p.237-249 |
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| creator | Miyachi, Yoshiki Wee, Siew-Lin Chen, Li-Kuang Grumet, F.Carl Bowman, Robert J. Taurog, Joel D. |
| description | A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27−; 2) six families, including 20 B27+ and 14 B27− individuals; and 3) B27+ and B27− variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30–104%, while lysis of B27+,B27M2− targets was 4–22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ⩽10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis. |
| doi_str_mv | 10.1016/0198-8859(84)90089-2 |
| format | Article |
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This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27−; 2) six families, including 20 B27+ and 14 B27− individuals; and 3) B27+ and B27− variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30–104%, while lysis of B27+,B27M2− targets was 4–22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ⩽10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/0198-8859(84)90089-2</identifier><identifier>PMID: 6206036</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibodies, Monoclonal - immunology ; Binding, Competitive ; Clone Cells - immunology ; Epitopes - immunology ; HLA Antigens - immunology ; HLA-B27 Antigen ; Humans ; Spondylitis, Ankylosing - immunology ; T-Lymphocytes, Cytotoxic - immunology ; Uveitis, Anterior - immunology</subject><ispartof>Human immunology, 1984-08, Vol.10 (4), p.237-249</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-1b0f79b6d3e19a9c3173c310a98814862e4ad66d72e5653ace7a9e4c78bb21103</citedby><cites>FETCH-LOGICAL-c303t-1b0f79b6d3e19a9c3173c310a98814862e4ad66d72e5653ace7a9e4c78bb21103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0198-8859(84)90089-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6206036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyachi, Yoshiki</creatorcontrib><creatorcontrib>Wee, Siew-Lin</creatorcontrib><creatorcontrib>Chen, Li-Kuang</creatorcontrib><creatorcontrib>Grumet, F.Carl</creatorcontrib><creatorcontrib>Bowman, Robert J.</creatorcontrib><creatorcontrib>Taurog, Joel D.</creatorcontrib><title>A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27−; 2) six families, including 20 B27+ and 14 B27− individuals; and 3) B27+ and B27− variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30–104%, while lysis of B27+,B27M2− targets was 4–22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ⩽10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>Binding, Competitive</subject><subject>Clone Cells - immunology</subject><subject>Epitopes - immunology</subject><subject>HLA Antigens - immunology</subject><subject>HLA-B27 Antigen</subject><subject>Humans</subject><subject>Spondylitis, Ankylosing - immunology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Uveitis, Anterior - immunology</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVISTeb_oMUdArNwa3G0urjEtgs-YKF9pCchSyPExV_1bID7q-vtrvkmFxmYN5nZuAh5BzYd2AgfzAwOtN6Zb5pcWkY0ybLj8gCtDIZgJTHZPGGfCanMf5mjCmmxAk5kTmTjMsF-bWmfh67eh6Dpy9T41r6SOu56V-6NEfq665FWoaqwgHbMbi6numAvntuw9_QPtP77Tq7zhWNUzHOPcYz8qlydcQvh74kT7c3j5v7bPvz7mGz3maeMz5mULBKmUKWHME44zkongpzRmsQWuYoXCllqXJcyRV3HpUzKLzSRZEDML4kF_u7_dD9mTCOtgnRY127FrspWg058GTlQzBRRgsQCRR70A9djANWth9C44bZArM743an0-50Wi3sf-M2T2tfD_enosHybemgOOVX-xyTjdeAg40-YOuxDMnjaMsuvP_gHzX8jvc</recordid><startdate>198408</startdate><enddate>198408</enddate><creator>Miyachi, Yoshiki</creator><creator>Wee, Siew-Lin</creator><creator>Chen, Li-Kuang</creator><creator>Grumet, F.Carl</creator><creator>Bowman, Robert J.</creator><creator>Taurog, Joel D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198408</creationdate><title>A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes</title><author>Miyachi, Yoshiki ; Wee, Siew-Lin ; Chen, Li-Kuang ; Grumet, F.Carl ; Bowman, Robert J. ; Taurog, Joel D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-1b0f79b6d3e19a9c3173c310a98814862e4ad66d72e5653ace7a9e4c78bb21103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>Binding, Competitive</topic><topic>Clone Cells - immunology</topic><topic>Epitopes - immunology</topic><topic>HLA Antigens - immunology</topic><topic>HLA-B27 Antigen</topic><topic>Humans</topic><topic>Spondylitis, Ankylosing - immunology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Uveitis, Anterior - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyachi, Yoshiki</creatorcontrib><creatorcontrib>Wee, Siew-Lin</creatorcontrib><creatorcontrib>Chen, Li-Kuang</creatorcontrib><creatorcontrib>Grumet, F.Carl</creatorcontrib><creatorcontrib>Bowman, Robert J.</creatorcontrib><creatorcontrib>Taurog, Joel D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyachi, Yoshiki</au><au>Wee, Siew-Lin</au><au>Chen, Li-Kuang</au><au>Grumet, F.Carl</au><au>Bowman, Robert J.</au><au>Taurog, Joel D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>1984-08</date><risdate>1984</risdate><volume>10</volume><issue>4</issue><spage>237</spage><epage>249</epage><pages>237-249</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27−; 2) six families, including 20 B27+ and 14 B27− individuals; and 3) B27+ and B27− variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30–104%, while lysis of B27+,B27M2− targets was 4–22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ⩽10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6206036</pmid><doi>10.1016/0198-8859(84)90089-2</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Human immunology, 1984-08, Vol.10 (4), p.237-249 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - immunology Binding, Competitive Clone Cells - immunology Epitopes - immunology HLA Antigens - immunology HLA-B27 Antigen Humans Spondylitis, Ankylosing - immunology T-Lymphocytes, Cytotoxic - immunology Uveitis, Anterior - immunology |
| title | A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes |
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