A fast and simple assay for busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry using turbulent flow online extraction technology
Busulfan is used in myeloablative preparation regimens for hematopoietic bone marrow transplantation. Due to its narrow therapeutic range therapeutic drug monitoring of busulfan is recommended. In this study a fast and simple method for measuring busulfan in serum or plasma by liquid chromatography–...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-12, Vol.878 (31), p.3255-3258 |
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| creator | Bunch, Dustin R. Heideloff, Courtney Ritchie, James C. Wang, Sihe |
| description | Busulfan is used in myeloablative preparation regimens for hematopoietic bone marrow transplantation. Due to its narrow therapeutic range therapeutic drug monitoring of busulfan is recommended. In this study a fast and simple method for measuring busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed utilizing turbulent flow online extraction technology. Serum or plasma was mixed with acetonitrile containing d
8-busulfan. After centrifugation the supernatant was injected onto a turbulent flow preparatory column then transferred to a C18 analytical column monitored by a tandem mass spectrometer set at positive electrospray ionization. The analytical cycle time was 4.0
min. The method was linear from 0.15 to 41.90
μmol/L with an accuracy of 87.9–103.0%. Inter- and intra-assay CVs across four concentration levels were 2.1–7.8%. No significant carryover or ion suppression was observed. No interference was observed from commercial control materials containing more than 100 compounds. Comparison with a well established LC–MS/MS method using patient specimens (
n
=
45) showed a mean bias 1.3% with Deming regression of slope 1.02, intercept −0.02
μmol/L, and a linear correlation coefficient 0.9883. The LC–MS/MS method coupled with turbulent flow online sample cleaning technology described here offers reliable busulfan quantitation in serum or plasma with minimum manual sample preparation and was fully validated for clinical use. |
| doi_str_mv | 10.1016/j.jchromb.2010.10.003 |
| format | Article |
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8-busulfan. After centrifugation the supernatant was injected onto a turbulent flow preparatory column then transferred to a C18 analytical column monitored by a tandem mass spectrometer set at positive electrospray ionization. The analytical cycle time was 4.0
min. The method was linear from 0.15 to 41.90
μmol/L with an accuracy of 87.9–103.0%. Inter- and intra-assay CVs across four concentration levels were 2.1–7.8%. No significant carryover or ion suppression was observed. No interference was observed from commercial control materials containing more than 100 compounds. Comparison with a well established LC–MS/MS method using patient specimens (
n
=
45) showed a mean bias 1.3% with Deming regression of slope 1.02, intercept −0.02
μmol/L, and a linear correlation coefficient 0.9883. The LC–MS/MS method coupled with turbulent flow online sample cleaning technology described here offers reliable busulfan quantitation in serum or plasma with minimum manual sample preparation and was fully validated for clinical use.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2010.10.003</identifier><identifier>PMID: 21030324</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetonitriles - chemistry ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Busulfan ; Busulfan - blood ; Chemical Fractionation - methods ; Chromatography ; Chromatography, Liquid - methods ; Drug Monitoring ; Fluid dynamics ; Fluid flow ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Humans ; Least-Squares Analysis ; Liquid chromatography ; Medical sciences ; On-line systems ; Online ; Pharmacology. Drug treatments ; Reproducibility of Results ; Serums ; Spectrometry, Mass, Electrospray Ionization ; Tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Turbulence ; Turbulent flow</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-12, Vol.878 (31), p.3255-3258</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-586b41e90fe62ac1142e95f006f3c73c10f62dfee54cb9a7dd9454e125bb1a4e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023210006379$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23504954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21030324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bunch, Dustin R.</creatorcontrib><creatorcontrib>Heideloff, Courtney</creatorcontrib><creatorcontrib>Ritchie, James C.</creatorcontrib><creatorcontrib>Wang, Sihe</creatorcontrib><title>A fast and simple assay for busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry using turbulent flow online extraction technology</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Busulfan is used in myeloablative preparation regimens for hematopoietic bone marrow transplantation. Due to its narrow therapeutic range therapeutic drug monitoring of busulfan is recommended. In this study a fast and simple method for measuring busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed utilizing turbulent flow online extraction technology. Serum or plasma was mixed with acetonitrile containing d
8-busulfan. After centrifugation the supernatant was injected onto a turbulent flow preparatory column then transferred to a C18 analytical column monitored by a tandem mass spectrometer set at positive electrospray ionization. The analytical cycle time was 4.0
min. The method was linear from 0.15 to 41.90
μmol/L with an accuracy of 87.9–103.0%. Inter- and intra-assay CVs across four concentration levels were 2.1–7.8%. No significant carryover or ion suppression was observed. No interference was observed from commercial control materials containing more than 100 compounds. Comparison with a well established LC–MS/MS method using patient specimens (
n
=
45) showed a mean bias 1.3% with Deming regression of slope 1.02, intercept −0.02
μmol/L, and a linear correlation coefficient 0.9883. The LC–MS/MS method coupled with turbulent flow online sample cleaning technology described here offers reliable busulfan quantitation in serum or plasma with minimum manual sample preparation and was fully validated for clinical use.</description><subject>Acetonitriles - chemistry</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Busulfan</subject><subject>Busulfan - blood</subject><subject>Chemical Fractionation - methods</subject><subject>Chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Drug Monitoring</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Least-Squares Analysis</subject><subject>Liquid chromatography</subject><subject>Medical sciences</subject><subject>On-line systems</subject><subject>Online</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Serums</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Turbulence</subject><subject>Turbulent flow</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTuOFDEQhlsIxC4LRwA5QZDM4EfbPR2h1YqXtBIJSGSW212e8cht9_oBdMYdiLkcJ8GzM0AGka3yV3-V_DXNY4LXBBPxYr_e610M07Cm-La2xpjdac7JpmMr1olPd-udd3iFKaNnzYOU9hiTDnfsfnNGCWaY0fa8-XGJjEoZKT-iZKfZAVIpqQWZENFQUnFGeWQ9ShDLhGpxdipNCg0Lcvam2BHdrqFy2EY175af377nGgYTmmoQSjPoXN8hxwWVZP0W5RKH4sBnZFz4goJ31gOCrzkqnW3wKIPe-eDCdnnY3DPKJXh0Oi-aj69ffbh6u7p-_-bd1eX1Srdc5BXfiKEl0GMDgipNSEuh5wZjYZjumCbYCDoaAN7qoVfdOPYtb4FQPgxEtcAummfH3DmGmwIpy8kmDc4pD6EkuSGUME56Vsnn_ySJ6GosE4JWlB9RHUNKEYyco51UXCTB8uBQ7uXJoTw4PJSrw9r35DSiDBOMf7p-S6vA0xOgklbOROW1TX85xnHb8wP38shB_brPFqJM2oLXMNpYrcgx2P-s8gu85sH1</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Bunch, Dustin R.</creator><creator>Heideloff, Courtney</creator><creator>Ritchie, James C.</creator><creator>Wang, Sihe</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20101201</creationdate><title>A fast and simple assay for busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry using turbulent flow online extraction technology</title><author>Bunch, Dustin R. ; Heideloff, Courtney ; Ritchie, James C. ; Wang, Sihe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-586b41e90fe62ac1142e95f006f3c73c10f62dfee54cb9a7dd9454e125bb1a4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetonitriles - chemistry</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Busulfan</topic><topic>Busulfan - blood</topic><topic>Chemical Fractionation - methods</topic><topic>Chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Drug Monitoring</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Least-Squares Analysis</topic><topic>Liquid chromatography</topic><topic>Medical sciences</topic><topic>On-line systems</topic><topic>Online</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Serums</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Turbulence</topic><topic>Turbulent flow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bunch, Dustin R.</creatorcontrib><creatorcontrib>Heideloff, Courtney</creatorcontrib><creatorcontrib>Ritchie, James C.</creatorcontrib><creatorcontrib>Wang, Sihe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bunch, Dustin R.</au><au>Heideloff, Courtney</au><au>Ritchie, James C.</au><au>Wang, Sihe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A fast and simple assay for busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry using turbulent flow online extraction technology</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>878</volume><issue>31</issue><spage>3255</spage><epage>3258</epage><pages>3255-3258</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Busulfan is used in myeloablative preparation regimens for hematopoietic bone marrow transplantation. Due to its narrow therapeutic range therapeutic drug monitoring of busulfan is recommended. In this study a fast and simple method for measuring busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed utilizing turbulent flow online extraction technology. Serum or plasma was mixed with acetonitrile containing d
8-busulfan. After centrifugation the supernatant was injected onto a turbulent flow preparatory column then transferred to a C18 analytical column monitored by a tandem mass spectrometer set at positive electrospray ionization. The analytical cycle time was 4.0
min. The method was linear from 0.15 to 41.90
μmol/L with an accuracy of 87.9–103.0%. Inter- and intra-assay CVs across four concentration levels were 2.1–7.8%. No significant carryover or ion suppression was observed. No interference was observed from commercial control materials containing more than 100 compounds. Comparison with a well established LC–MS/MS method using patient specimens (
n
=
45) showed a mean bias 1.3% with Deming regression of slope 1.02, intercept −0.02
μmol/L, and a linear correlation coefficient 0.9883. The LC–MS/MS method coupled with turbulent flow online sample cleaning technology described here offers reliable busulfan quantitation in serum or plasma with minimum manual sample preparation and was fully validated for clinical use.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21030324</pmid><doi>10.1016/j.jchromb.2010.10.003</doi><tpages>4</tpages></addata></record> |
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| subjects | Acetonitriles - chemistry Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Busulfan Busulfan - blood Chemical Fractionation - methods Chromatography Chromatography, Liquid - methods Drug Monitoring Fluid dynamics Fluid flow Fundamental and applied biological sciences. Psychology General pharmacology Humans Least-Squares Analysis Liquid chromatography Medical sciences On-line systems Online Pharmacology. Drug treatments Reproducibility of Results Serums Spectrometry, Mass, Electrospray Ionization Tandem mass spectrometry Tandem Mass Spectrometry - methods Turbulence Turbulent flow |
| title | A fast and simple assay for busulfan in serum or plasma by liquid chromatography–tandem mass spectrometry using turbulent flow online extraction technology |
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