Large-scale purification of porcine calpain I and calpain II and comparison of proteolytic fragments of their subunits

Large-scale purification of calpain [Ca2+-dependent cysteine proteinase; EC 3.4.22.17] from porcine tissues is described. The methods used included chromatographies on DEAE-cellulose, Ultrogel AcA 34, Blue Sepharose CL-6B, and DEAE Bio-Gel A which yielded homogeneous enzyme proteins: 27.0 mg of calp...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1984-06, Vol.95 (6), p.1759-1766
Hauptverfasser:	KITAHARA, A, SASAKI, T, KIKUCHI, T, YUMOTO, N, YOSHIMURA, N, HATANAKA, M, MURACHI, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biotechnology Brain - enzymology Calcium - analysis Calpain electrophoresis Endopeptidases - isolation & purification Endopeptidases - metabolism Enzyme engineering Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Improved methods for extraction and purification of enzymes Isoenzymes - isolation & purification Isoenzymes - metabolism Kinetics Liver - enzymology Macromolecular Substances Methods. Procedures. Technologies Molecular Weight Myocardium - enzymology Peptide Fragments - analysis pigs Swine
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