A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein
Sensitive immunochemical assays were used to measure the mass and rate of synthesis of malic enzyme protein in wild-type and Mod-1n mutant mice fed a high carbohydrate/low fat diet supplemented with thyroid hormone. Malic enzyme activity in the fed, wild-type mice was 100-fold higher than in starved...
Gespeichert in:
Veröffentlicht in: | Biochemistry (Easton) 1984-07, Vol.23 (15), p.3454-3459 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 3459 |
---|---|
container_issue | 15 |
container_start_page | 3454 |
container_title | Biochemistry (Easton) |
container_volume | 23 |
creator | Glynias, Manuel J Morris, Sidney M Fantozzi, Dominic A Winberry, Larry K Back, Donald W Fisch, Judith E Goodridge, Alan G |
description | Sensitive immunochemical assays were used to measure the mass and rate of synthesis of malic enzyme protein in wild-type and Mod-1n mutant mice fed a high carbohydrate/low fat diet supplemented with thyroid hormone. Malic enzyme activity in the fed, wild-type mice was 100-fold higher than in starved, wild-type mice. Neither activity, mass, nor synthesis of malic enzyme could be detected in fed, mutant mice. However, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase responded to these dietary manipulations with normal or supranormal increases in activities, respectively, in mutant mice. A cDNA clone containing an almost complete copy of the mRNA for malic enzyme from duck liver was used to analyze poly(A+) RNA from C57BL/6J-DBA/2J hybrid mice that had been fasted and refed a high carbohydrate/low fat diet supplemented with thyroid hormone. The 32P-cDNA probe hybridized to two RNAs of 2250 and 2950 nucleotides. The same two RNAs were detected in RNA from starved mice except at much lower concentrations. A similar analysis of RNA from Mod-1n mice fed the high carbohydrate-thyroid diet also revealed two hybridizing RNAs but each was 700-800 nucleotides longer than its counterpart in wild-type mice. The abundance of malic enzyme mRNA in the fed, mutant mice was about the same as that in fed, wild-type mice. The mutant malic enzyme mRNAs also were present in RNA from starved mice but at much lower concentrations. These results suggest that the mutation responsible for the Mod-1n phenotype is in the structural gene for malic enzyme. |
doi_str_mv | 10.1021/bi00310a011 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81202553</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>13904377</sourcerecordid><originalsourceid>FETCH-LOGICAL-a329t-211893c1c9d5a05a800f5f86e90cde5e2df822936e506a58cd01c47be9f4c32a3</originalsourceid><addsrcrecordid>eNqFkVFrFDEUhYModVt98lnIg2hFRm-Syczkcd1qFWoVrb6GbHJHp51J1iQLXf-If9eUXRYLgk83l_NxziWHkEcMXjLg7NVyABAMDDB2h8yY5FDVSsm7ZAYATcVVA_fJYUqXZa2hrQ_IQSPrtpNsRn7PqR2DR0ftyfmc9iFSt7ZXdDLjYCn6X5sJqcOMNidqlj7EoowbOpr4HW9T0-fzeaKDp4amHE15hJ5Og0V6_CG4ivnnNP8wmbqAifqQKV6vIqZ022UVQ8bBPyD3ejMmfLibR-Tr2zcXi3fV2cfT94v5WWUEV7nijHVKWGaVkwak6QB62XcNKrAOJXLXd5wr0aCExsjOOmC2bpeo-toKbsQRebr1Lbk_15iynoZkcRyNx7BOumMcuJTivyATCmrRtgV8sQVtDClF7PUqDpOJG81A3_Sl_-qr0I93tuvlhG7P7goq-pOdbpI1Yx-Nt0PaYwp4ybwJrbbYkDJe72UTr3TTilbqi09f9Mnrb2xRd6caCv9syxub9GVYR18--Z8H_gEF-rfQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13904377</pqid></control><display><type>article</type><title>A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Glynias, Manuel J ; Morris, Sidney M ; Fantozzi, Dominic A ; Winberry, Larry K ; Back, Donald W ; Fisch, Judith E ; Goodridge, Alan G</creator><creatorcontrib>Glynias, Manuel J ; Morris, Sidney M ; Fantozzi, Dominic A ; Winberry, Larry K ; Back, Donald W ; Fisch, Judith E ; Goodridge, Alan G</creatorcontrib><description>Sensitive immunochemical assays were used to measure the mass and rate of synthesis of malic enzyme protein in wild-type and Mod-1n mutant mice fed a high carbohydrate/low fat diet supplemented with thyroid hormone. Malic enzyme activity in the fed, wild-type mice was 100-fold higher than in starved, wild-type mice. Neither activity, mass, nor synthesis of malic enzyme could be detected in fed, mutant mice. However, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase responded to these dietary manipulations with normal or supranormal increases in activities, respectively, in mutant mice. A cDNA clone containing an almost complete copy of the mRNA for malic enzyme from duck liver was used to analyze poly(A+) RNA from C57BL/6J-DBA/2J hybrid mice that had been fasted and refed a high carbohydrate/low fat diet supplemented with thyroid hormone. The 32P-cDNA probe hybridized to two RNAs of 2250 and 2950 nucleotides. The same two RNAs were detected in RNA from starved mice except at much lower concentrations. A similar analysis of RNA from Mod-1n mice fed the high carbohydrate-thyroid diet also revealed two hybridizing RNAs but each was 700-800 nucleotides longer than its counterpart in wild-type mice. The abundance of malic enzyme mRNA in the fed, mutant mice was about the same as that in fed, wild-type mice. The mutant malic enzyme mRNAs also were present in RNA from starved mice but at much lower concentrations. These results suggest that the mutation responsible for the Mod-1n phenotype is in the structural gene for malic enzyme.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00310a011</identifier><identifier>PMID: 6547851</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Cloning, Molecular ; Crosses, Genetic ; DNA - metabolism ; Ducks - genetics ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Liver - enzymology ; Malate Dehydrogenase - genetics ; Mice ; Mice, Inbred Strains ; Mice, Mutant Strains - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Weight ; RNA, Messenger - genetics ; Species Specificity</subject><ispartof>Biochemistry (Easton), 1984-07, Vol.23 (15), p.3454-3459</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a329t-211893c1c9d5a05a800f5f86e90cde5e2df822936e506a58cd01c47be9f4c32a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00310a011$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00310a011$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9023777$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6547851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Glynias, Manuel J</creatorcontrib><creatorcontrib>Morris, Sidney M</creatorcontrib><creatorcontrib>Fantozzi, Dominic A</creatorcontrib><creatorcontrib>Winberry, Larry K</creatorcontrib><creatorcontrib>Back, Donald W</creatorcontrib><creatorcontrib>Fisch, Judith E</creatorcontrib><creatorcontrib>Goodridge, Alan G</creatorcontrib><title>A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Sensitive immunochemical assays were used to measure the mass and rate of synthesis of malic enzyme protein in wild-type and Mod-1n mutant mice fed a high carbohydrate/low fat diet supplemented with thyroid hormone. Malic enzyme activity in the fed, wild-type mice was 100-fold higher than in starved, wild-type mice. Neither activity, mass, nor synthesis of malic enzyme could be detected in fed, mutant mice. However, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase responded to these dietary manipulations with normal or supranormal increases in activities, respectively, in mutant mice. A cDNA clone containing an almost complete copy of the mRNA for malic enzyme from duck liver was used to analyze poly(A+) RNA from C57BL/6J-DBA/2J hybrid mice that had been fasted and refed a high carbohydrate/low fat diet supplemented with thyroid hormone. The 32P-cDNA probe hybridized to two RNAs of 2250 and 2950 nucleotides. The same two RNAs were detected in RNA from starved mice except at much lower concentrations. A similar analysis of RNA from Mod-1n mice fed the high carbohydrate-thyroid diet also revealed two hybridizing RNAs but each was 700-800 nucleotides longer than its counterpart in wild-type mice. The abundance of malic enzyme mRNA in the fed, mutant mice was about the same as that in fed, wild-type mice. The mutant malic enzyme mRNAs also were present in RNA from starved mice but at much lower concentrations. These results suggest that the mutation responsible for the Mod-1n phenotype is in the structural gene for malic enzyme.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Crosses, Genetic</subject><subject>DNA - metabolism</subject><subject>Ducks - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Liver - enzymology</subject><subject>Malate Dehydrogenase - genetics</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Mutant Strains - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Weight</subject><subject>RNA, Messenger - genetics</subject><subject>Species Specificity</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrFDEUhYModVt98lnIg2hFRm-Syczkcd1qFWoVrb6GbHJHp51J1iQLXf-If9eUXRYLgk83l_NxziWHkEcMXjLg7NVyABAMDDB2h8yY5FDVSsm7ZAYATcVVA_fJYUqXZa2hrQ_IQSPrtpNsRn7PqR2DR0ftyfmc9iFSt7ZXdDLjYCn6X5sJqcOMNidqlj7EoowbOpr4HW9T0-fzeaKDp4amHE15hJ5Og0V6_CG4ivnnNP8wmbqAifqQKV6vIqZ022UVQ8bBPyD3ejMmfLibR-Tr2zcXi3fV2cfT94v5WWUEV7nijHVKWGaVkwak6QB62XcNKrAOJXLXd5wr0aCExsjOOmC2bpeo-toKbsQRebr1Lbk_15iynoZkcRyNx7BOumMcuJTivyATCmrRtgV8sQVtDClF7PUqDpOJG81A3_Sl_-qr0I93tuvlhG7P7goq-pOdbpI1Yx-Nt0PaYwp4ybwJrbbYkDJe72UTr3TTilbqi09f9Mnrb2xRd6caCv9syxub9GVYR18--Z8H_gEF-rfQ</recordid><startdate>19840717</startdate><enddate>19840717</enddate><creator>Glynias, Manuel J</creator><creator>Morris, Sidney M</creator><creator>Fantozzi, Dominic A</creator><creator>Winberry, Larry K</creator><creator>Back, Donald W</creator><creator>Fisch, Judith E</creator><creator>Goodridge, Alan G</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19840717</creationdate><title>A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein</title><author>Glynias, Manuel J ; Morris, Sidney M ; Fantozzi, Dominic A ; Winberry, Larry K ; Back, Donald W ; Fisch, Judith E ; Goodridge, Alan G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a329t-211893c1c9d5a05a800f5f86e90cde5e2df822936e506a58cd01c47be9f4c32a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Crosses, Genetic</topic><topic>DNA - metabolism</topic><topic>Ducks - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Liver - enzymology</topic><topic>Malate Dehydrogenase - genetics</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Mutant Strains - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Weight</topic><topic>RNA, Messenger - genetics</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Glynias, Manuel J</creatorcontrib><creatorcontrib>Morris, Sidney M</creatorcontrib><creatorcontrib>Fantozzi, Dominic A</creatorcontrib><creatorcontrib>Winberry, Larry K</creatorcontrib><creatorcontrib>Back, Donald W</creatorcontrib><creatorcontrib>Fisch, Judith E</creatorcontrib><creatorcontrib>Goodridge, Alan G</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Glynias, Manuel J</au><au>Morris, Sidney M</au><au>Fantozzi, Dominic A</au><au>Winberry, Larry K</au><au>Back, Donald W</au><au>Fisch, Judith E</au><au>Goodridge, Alan G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1984-07-17</date><risdate>1984</risdate><volume>23</volume><issue>15</issue><spage>3454</spage><epage>3459</epage><pages>3454-3459</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Sensitive immunochemical assays were used to measure the mass and rate of synthesis of malic enzyme protein in wild-type and Mod-1n mutant mice fed a high carbohydrate/low fat diet supplemented with thyroid hormone. Malic enzyme activity in the fed, wild-type mice was 100-fold higher than in starved, wild-type mice. Neither activity, mass, nor synthesis of malic enzyme could be detected in fed, mutant mice. However, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase responded to these dietary manipulations with normal or supranormal increases in activities, respectively, in mutant mice. A cDNA clone containing an almost complete copy of the mRNA for malic enzyme from duck liver was used to analyze poly(A+) RNA from C57BL/6J-DBA/2J hybrid mice that had been fasted and refed a high carbohydrate/low fat diet supplemented with thyroid hormone. The 32P-cDNA probe hybridized to two RNAs of 2250 and 2950 nucleotides. The same two RNAs were detected in RNA from starved mice except at much lower concentrations. A similar analysis of RNA from Mod-1n mice fed the high carbohydrate-thyroid diet also revealed two hybridizing RNAs but each was 700-800 nucleotides longer than its counterpart in wild-type mice. The abundance of malic enzyme mRNA in the fed, mutant mice was about the same as that in fed, wild-type mice. The mutant malic enzyme mRNAs also were present in RNA from starved mice but at much lower concentrations. These results suggest that the mutation responsible for the Mod-1n phenotype is in the structural gene for malic enzyme.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>6547851</pmid><doi>10.1021/bi00310a011</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0006-2960 |
ispartof | Biochemistry (Easton), 1984-07, Vol.23 (15), p.3454-3459 |
issn | 0006-2960 1520-4995 |
language | eng |
recordid | cdi_proquest_miscellaneous_81202553 |
source | MEDLINE; American Chemical Society Journals |
subjects | Animals Biological and medical sciences Cloning, Molecular Crosses, Genetic DNA - metabolism Ducks - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Liver - enzymology Malate Dehydrogenase - genetics Mice Mice, Inbred Strains Mice, Mutant Strains - genetics Molecular and cellular biology Molecular genetics Molecular Weight RNA, Messenger - genetics Species Specificity |
title | A cloned cDNA for duck malic enzyme detects abnormally large malic enzyme mRNAs in a strain of mice (Mod-1n) that does not express malic enzyme protein |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A03%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20cloned%20cDNA%20for%20duck%20malic%20enzyme%20detects%20abnormally%20large%20malic%20enzyme%20mRNAs%20in%20a%20strain%20of%20mice%20(Mod-1n)%20that%20does%20not%20express%20malic%20enzyme%20protein&rft.jtitle=Biochemistry%20(Easton)&rft.au=Glynias,%20Manuel%20J&rft.date=1984-07-17&rft.volume=23&rft.issue=15&rft.spage=3454&rft.epage=3459&rft.pages=3454-3459&rft.issn=0006-2960&rft.eissn=1520-4995&rft_id=info:doi/10.1021/bi00310a011&rft_dat=%3Cproquest_cross%3E13904377%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13904377&rft_id=info:pmid/6547851&rfr_iscdi=true |