Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses
The major component of the core structure of avian sarcoma leukosis viruses is a 27 kD molecular weight polypeptide, p27. Spleen cells from mice immunized with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fused with mouse myeloma cells (SP2/0), and hybridoma cell lines producing monocl...
Gespeichert in:
| Veröffentlicht in: | Hybridoma 1984, Vol.3 (1), p.25-31 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 31 |
|---|---|
| container_issue | 1 |
| container_start_page | 25 |
| container_title | Hybridoma |
| container_volume | 3 |
| creator | Parsons, S J Wilson, L D Ely, C M Parsons, J T Benjamin, D C |
| description | The major component of the core structure of avian sarcoma leukosis viruses is a 27 kD molecular weight polypeptide, p27. Spleen cells from mice immunized with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fused with mouse myeloma cells (SP2/0), and hybridoma cell lines producing monoclonal antibodies to p27 were isolated. The monoclonal antibodies were all of the IgG1 subclass with kappa light chains. These antibodies immunoprecipitated p27 and its precursor proteins from extracts of RSV-transformed cells. Reciprocal competitive binding experiments defined five nonoverlapping antigenic determinants within p27. The monoclonal antibodies also immunoprecipitated the transforming protein, p110gag-myc, from avian myelocytomatosis virus transformed cells. Their usefulness in studies of virion maturation and viral oncogenesis is discussed. |
| doi_str_mv | 10.1089/hyb.1984.3.25 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81199756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81199756</sourcerecordid><originalsourceid>FETCH-LOGICAL-c275t-ac2006541faf0bc45b18bce70cd1eb8cb00ea4dce025852162545a1f49d5533d3</originalsourceid><addsrcrecordid>eNqF0M9LwzAUwPEgypzTo0chJ0_rTNKmSY8i_oKJFwfeQpK-sEjX1KQd7L-3Y0O8CQ_eO3x4hy9C15QsKJHV3XpnFrSSxSJfMH6CpoQJlhVcfJ7-uc_RRUpfhBDOCjFBk5IRXsl8ilZvoQ22Ca1usG57b0LtIeHUgfXOW-xCxP0a8NZHH1rchWbXQdf7Gua4Y2KOg8N663WLI_QxjGxIkC7RmdNNgqvjnqHV0-PHw0u2fH9-fbhfZpYJ3mfaMkJKXlCnHTG24IZKY0EQW1Mw0hpCQBe1BcK45IyWjBdcU1dUNed5XuczdHv428XwPUDq1cYnC02jWwhDUpLSqhK8_BfSXFIxzgizA7QxpBTBqS76jY47RYna91Zjb7XvrXLF-Ohvjo8Hs4H6Vx8D5z_eIHx8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13817817</pqid></control><display><type>article</type><title>Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>Parsons, S J ; Wilson, L D ; Ely, C M ; Parsons, J T ; Benjamin, D C</creator><creatorcontrib>Parsons, S J ; Wilson, L D ; Ely, C M ; Parsons, J T ; Benjamin, D C</creatorcontrib><description>The major component of the core structure of avian sarcoma leukosis viruses is a 27 kD molecular weight polypeptide, p27. Spleen cells from mice immunized with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fused with mouse myeloma cells (SP2/0), and hybridoma cell lines producing monoclonal antibodies to p27 were isolated. The monoclonal antibodies were all of the IgG1 subclass with kappa light chains. These antibodies immunoprecipitated p27 and its precursor proteins from extracts of RSV-transformed cells. Reciprocal competitive binding experiments defined five nonoverlapping antigenic determinants within p27. The monoclonal antibodies also immunoprecipitated the transforming protein, p110gag-myc, from avian myelocytomatosis virus transformed cells. Their usefulness in studies of virion maturation and viral oncogenesis is discussed.</description><identifier>ISSN: 0272-457X</identifier><identifier>EISSN: 0272-457X</identifier><identifier>DOI: 10.1089/hyb.1984.3.25</identifier><identifier>PMID: 6205983</identifier><language>eng</language><publisher>United States</publisher><subject>Alpharetrovirus - immunology ; Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Antibody Specificity ; Antigens, Viral - immunology ; Binding, Competitive ; Cell Transformation, Viral ; Chick Embryo ; core protein ; Epitopes - immunology ; Hybridomas - immunology ; Mice ; monoclonal antibodies ; Oncogene Protein pp60(v-src) ; Retroviridae Proteins ; retrovirus (avian) ; Viral Proteins - immunology</subject><ispartof>Hybridoma, 1984, Vol.3 (1), p.25-31</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c275t-ac2006541faf0bc45b18bce70cd1eb8cb00ea4dce025852162545a1f49d5533d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3028,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6205983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parsons, S J</creatorcontrib><creatorcontrib>Wilson, L D</creatorcontrib><creatorcontrib>Ely, C M</creatorcontrib><creatorcontrib>Parsons, J T</creatorcontrib><creatorcontrib>Benjamin, D C</creatorcontrib><title>Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>The major component of the core structure of avian sarcoma leukosis viruses is a 27 kD molecular weight polypeptide, p27. Spleen cells from mice immunized with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fused with mouse myeloma cells (SP2/0), and hybridoma cell lines producing monoclonal antibodies to p27 were isolated. The monoclonal antibodies were all of the IgG1 subclass with kappa light chains. These antibodies immunoprecipitated p27 and its precursor proteins from extracts of RSV-transformed cells. Reciprocal competitive binding experiments defined five nonoverlapping antigenic determinants within p27. The monoclonal antibodies also immunoprecipitated the transforming protein, p110gag-myc, from avian myelocytomatosis virus transformed cells. Their usefulness in studies of virion maturation and viral oncogenesis is discussed.</description><subject>Alpharetrovirus - immunology</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Specificity</subject><subject>Antigens, Viral - immunology</subject><subject>Binding, Competitive</subject><subject>Cell Transformation, Viral</subject><subject>Chick Embryo</subject><subject>core protein</subject><subject>Epitopes - immunology</subject><subject>Hybridomas - immunology</subject><subject>Mice</subject><subject>monoclonal antibodies</subject><subject>Oncogene Protein pp60(v-src)</subject><subject>Retroviridae Proteins</subject><subject>retrovirus (avian)</subject><subject>Viral Proteins - immunology</subject><issn>0272-457X</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9LwzAUwPEgypzTo0chJ0_rTNKmSY8i_oKJFwfeQpK-sEjX1KQd7L-3Y0O8CQ_eO3x4hy9C15QsKJHV3XpnFrSSxSJfMH6CpoQJlhVcfJ7-uc_RRUpfhBDOCjFBk5IRXsl8ilZvoQ22Ca1usG57b0LtIeHUgfXOW-xCxP0a8NZHH1rchWbXQdf7Gua4Y2KOg8N663WLI_QxjGxIkC7RmdNNgqvjnqHV0-PHw0u2fH9-fbhfZpYJ3mfaMkJKXlCnHTG24IZKY0EQW1Mw0hpCQBe1BcK45IyWjBdcU1dUNed5XuczdHv428XwPUDq1cYnC02jWwhDUpLSqhK8_BfSXFIxzgizA7QxpBTBqS76jY47RYna91Zjb7XvrXLF-Ohvjo8Hs4H6Vx8D5z_eIHx8</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Parsons, S J</creator><creator>Wilson, L D</creator><creator>Ely, C M</creator><creator>Parsons, J T</creator><creator>Benjamin, D C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1984</creationdate><title>Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses</title><author>Parsons, S J ; Wilson, L D ; Ely, C M ; Parsons, J T ; Benjamin, D C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-ac2006541faf0bc45b18bce70cd1eb8cb00ea4dce025852162545a1f49d5533d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Alpharetrovirus - immunology</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Antibody Specificity</topic><topic>Antigens, Viral - immunology</topic><topic>Binding, Competitive</topic><topic>Cell Transformation, Viral</topic><topic>Chick Embryo</topic><topic>core protein</topic><topic>Epitopes - immunology</topic><topic>Hybridomas - immunology</topic><topic>Mice</topic><topic>monoclonal antibodies</topic><topic>Oncogene Protein pp60(v-src)</topic><topic>Retroviridae Proteins</topic><topic>retrovirus (avian)</topic><topic>Viral Proteins - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>Parsons, S J</creatorcontrib><creatorcontrib>Wilson, L D</creatorcontrib><creatorcontrib>Ely, C M</creatorcontrib><creatorcontrib>Parsons, J T</creatorcontrib><creatorcontrib>Benjamin, D C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Hybridoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parsons, S J</au><au>Wilson, L D</au><au>Ely, C M</au><au>Parsons, J T</au><au>Benjamin, D C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>1984</date><risdate>1984</risdate><volume>3</volume><issue>1</issue><spage>25</spage><epage>31</epage><pages>25-31</pages><issn>0272-457X</issn><eissn>0272-457X</eissn><abstract>The major component of the core structure of avian sarcoma leukosis viruses is a 27 kD molecular weight polypeptide, p27. Spleen cells from mice immunized with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV) were fused with mouse myeloma cells (SP2/0), and hybridoma cell lines producing monoclonal antibodies to p27 were isolated. The monoclonal antibodies were all of the IgG1 subclass with kappa light chains. These antibodies immunoprecipitated p27 and its precursor proteins from extracts of RSV-transformed cells. Reciprocal competitive binding experiments defined five nonoverlapping antigenic determinants within p27. The monoclonal antibodies also immunoprecipitated the transforming protein, p110gag-myc, from avian myelocytomatosis virus transformed cells. Their usefulness in studies of virion maturation and viral oncogenesis is discussed.</abstract><cop>United States</cop><pmid>6205983</pmid><doi>10.1089/hyb.1984.3.25</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0272-457X |
| ispartof | Hybridoma, 1984, Vol.3 (1), p.25-31 |
| issn | 0272-457X 0272-457X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81199756 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Alpharetrovirus - immunology Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Antibody Specificity Antigens, Viral - immunology Binding, Competitive Cell Transformation, Viral Chick Embryo core protein Epitopes - immunology Hybridomas - immunology Mice monoclonal antibodies Oncogene Protein pp60(v-src) Retroviridae Proteins retrovirus (avian) Viral Proteins - immunology |
| title | Monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T04%3A59%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Monoclonal%20antibodies%20specific%20for%20the%20virion%20polypeptide,%20p27,%20of%20avian%20retroviruses&rft.jtitle=Hybridoma&rft.au=Parsons,%20S%20J&rft.date=1984&rft.volume=3&rft.issue=1&rft.spage=25&rft.epage=31&rft.pages=25-31&rft.issn=0272-457X&rft.eissn=0272-457X&rft_id=info:doi/10.1089/hyb.1984.3.25&rft_dat=%3Cproquest_cross%3E81199756%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13817817&rft_id=info:pmid/6205983&rfr_iscdi=true |