Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site

The synthesis and characterization of an octanucleotide, d(GGsAATTCC), containing the recognition sequence of the EcoRI restriction endonuclease with a phosphorothioate internucleotidic linkage at the cleavage site are described. Two approaches for the synthesis of the RP and SP diastereomers of thi...

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Veröffentlicht in:	Biochemistry (Easton) 1984-07, Vol.23 (15), p.3443-3453
Hauptverfasser:	Connolly, Bernard A, Potter, Barry V. L, Eckstein, Fritz, Pingoud, Alfred, Grotjahn, Lutz
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid Deoxyribonuclease EcoRI DNA Restriction Enzymes - metabolism endodeoxyribonuclease EcoRI Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Indicators and Reagents Magnetic Resonance Spectroscopy Mass Spectrometry mass spectroscopy N.M.R Oligodeoxyribonucleotides - chemical synthesis oligonucleotides Oligonucleotides - chemical synthesis Organothiophosphates Stereoisomerism Substrate Specificity
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