Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase
Growing interest in cholinergic neuronal systems has caused a resurgence of thiocholine histochemical studies. Reports from different laboratories refer to “modified” Koelle-Friedenwald procedures for the histochemical localization of acetylcholinesterase (AChE) but the modifications are seldom spec...
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| Veröffentlicht in: | Brain research bulletin 1984-01, Vol.12 (5), p.543-553 |
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| creator | van Ooteghem, Sullen A. Shipley, Michael T. |
| description | Growing interest in cholinergic neuronal systems has caused a resurgence of thiocholine histochemical studies. Reports from different laboratories refer to “modified” Koelle-Friedenwald procedures for the histochemical localization of acetylcholinesterase (AChE) but the modifications are seldom specified. A glance at the literature suggests that there is considerable variability in results obtained in similar brain structures. We report a detailed thiocholine protocol and observations on several experimental factors which affect the sensitivity, consistency and the discreteness of localization of AChE in histological material. Attention to these factors and adherence to the protocol described here has yielded consistent results in several species, and in pre- and post-natal material. The value of AChE staining in conjunction with immunocytochemical localization of cholineacetyltransferase to study cholinergic neural structures is discussed. |
| doi_str_mv | 10.1016/0361-9230(84)90170-9 |
| format | Article |
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Reports from different laboratories refer to “modified” Koelle-Friedenwald procedures for the histochemical localization of acetylcholinesterase (AChE) but the modifications are seldom specified. A glance at the literature suggests that there is considerable variability in results obtained in similar brain structures. We report a detailed thiocholine protocol and observations on several experimental factors which affect the sensitivity, consistency and the discreteness of localization of AChE in histological material. Attention to these factors and adherence to the protocol described here has yielded consistent results in several species, and in pre- and post-natal material. 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Reports from different laboratories refer to “modified” Koelle-Friedenwald procedures for the histochemical localization of acetylcholinesterase (AChE) but the modifications are seldom specified. A glance at the literature suggests that there is considerable variability in results obtained in similar brain structures. We report a detailed thiocholine protocol and observations on several experimental factors which affect the sensitivity, consistency and the discreteness of localization of AChE in histological material. Attention to these factors and adherence to the protocol described here has yielded consistent results in several species, and in pre- and post-natal material. The value of AChE staining in conjunction with immunocytochemical localization of cholineacetyltransferase to study cholinergic neural structures is discussed.</description><subject>Acetylcholinesterase - metabolism</subject><subject>Acetylcholinesterase, AChE</subject><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - enzymology</subject><subject>Central nervous system</subject><subject>Cholinergic Fibers - enzymology</subject><subject>Di-isopropylfluorophosphate, DFP</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histochemistry</subject><subject>Histocytochemistry - methods</subject><subject>Koelle method</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Thiocholine method</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0361-9230</issn><issn>1873-2747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-OFCEQh4nRrOPqG2jCwRg9tEI3zZ-Lidk4atzEi54JXV3YmO5mBWbN-Aa-tczOZI56IlDfr4D6CHnK2WvOuHzDOskb03bspRavDOOKNeYe2XCtuqZVQt0nmzPykDzK-QdjTOpeXpALKaRigm3In62DElOmznuEEtbvtExIM645lHAbyp66daQQ6z4XXGFPo79DPkecZ2y2KeCI6y83j3SqSIQJlwBupguWKY7Ux0TnWA_Cb1dCXA95B1j2M0xxDivWtsllfEweeDdnfHJaL8m37fuvVx-b6y8fPl29u25AcFWagfUDM1p0DFrBjfLKoETH_TAOvWgRjYTOSQmjGpADA6mFGxwwZNrrfuguyYtj35sUf-7q7XYJGepf3Ipxl63m3PSdaP8L8s5UUqgKiiMIKeac0NubFBaX9pYze1BlDx7swYPVwt6psqbGnp3674YFx3Po5KbWn5_qLtfx-eRWCPmMGcVbw0XF3h4xrEO7DZhshlBF4RhSNWrHGP79jr-SPLNn</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>van Ooteghem, Sullen A.</creator><creator>Shipley, Michael T.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase</title><author>van Ooteghem, Sullen A. ; Shipley, Michael T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b05b098430c24197f79e6ea1fbdb542ee96c3a66cd7be1c0c684abac0e08f85b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Acetylcholinesterase - metabolism</topic><topic>Acetylcholinesterase, AChE</topic><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - enzymology</topic><topic>Central nervous system</topic><topic>Cholinergic Fibers - enzymology</topic><topic>Di-isopropylfluorophosphate, DFP</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histochemistry</topic><topic>Histocytochemistry - methods</topic><topic>Koelle method</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Thiocholine method</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Ooteghem, Sullen A.</creatorcontrib><creatorcontrib>Shipley, Michael T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Ooteghem, Sullen A.</au><au>Shipley, Michael T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase</atitle><jtitle>Brain research bulletin</jtitle><addtitle>Brain Res Bull</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>12</volume><issue>5</issue><spage>543</spage><epage>553</epage><pages>543-553</pages><issn>0361-9230</issn><eissn>1873-2747</eissn><coden>BRBUDU</coden><abstract>Growing interest in cholinergic neuronal systems has caused a resurgence of thiocholine histochemical studies. Reports from different laboratories refer to “modified” Koelle-Friedenwald procedures for the histochemical localization of acetylcholinesterase (AChE) but the modifications are seldom specified. A glance at the literature suggests that there is considerable variability in results obtained in similar brain structures. We report a detailed thiocholine protocol and observations on several experimental factors which affect the sensitivity, consistency and the discreteness of localization of AChE in histological material. Attention to these factors and adherence to the protocol described here has yielded consistent results in several species, and in pre- and post-natal material. The value of AChE staining in conjunction with immunocytochemical localization of cholineacetyltransferase to study cholinergic neural structures is discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>6467040</pmid><doi>10.1016/0361-9230(84)90170-9</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0361-9230 |
| ispartof | Brain research bulletin, 1984-01, Vol.12 (5), p.543-553 |
| issn | 0361-9230 1873-2747 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholinesterase - metabolism Acetylcholinesterase, AChE Animals Biochemistry and metabolism Biological and medical sciences Brain - enzymology Central nervous system Cholinergic Fibers - enzymology Di-isopropylfluorophosphate, DFP Fundamental and applied biological sciences. Psychology Histochemistry Histocytochemistry - methods Koelle method Male Rats Rats, Inbred Strains Thiocholine method Vertebrates: nervous system and sense organs |
| title | Factors affecting the sensitivity and consistency of the Koelle-Friedenwald histochemical method for localization of acetylcholinesterase |
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