Gene Expression in Vitro from Deoxyribonucleic Acid of Bacteriophage T7
The in vitro synthesis of lysozyme and RNA polymerase directed by DNA of bacteriophage T7 starts with different lag periods after addition of the template. The difference in lag period before appearance of enzyme could be attributed to a difference in the time needed for transcription. Assuming that...
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| Veröffentlicht in: | The Journal of biological chemistry 1971-11, Vol.246 (22), p.6707-6712 |
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| container_title | The Journal of biological chemistry |
| container_volume | 246 |
| creator | Schweiger, M Herrlich, P Millette, R L |
| description | The in vitro synthesis of lysozyme and RNA polymerase directed by DNA of bacteriophage T7 starts with different lag periods after addition
of the template. The difference in lag period before appearance of enzyme could be attributed to a difference in the time
needed for transcription. Assuming that the difference in lag phase is caused by a difference in the distance between the
promoter and the corresponding gene, and taking into account the average rates of transcription and translation, the locations
of the polymerase gene and the lysozyme gene on the phage genome were estimated. The distance between the promoter and the
distal end of the lysozyme gene corresponds to an RNA of the molecular weight 2.2 x 10 6 . The polymerase gene is closer to the promoter. Transcription of T7 DNA by Escherichia coli RNA polymerase in vitro does indeed result in a polycistronic messenger RNA containing the information for synthesis of both RNA polymerase and lysozyme. |
| doi_str_mv | 10.1016/S0021-9258(19)45904-8 |
| format | Article |
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of the template. The difference in lag period before appearance of enzyme could be attributed to a difference in the time
needed for transcription. Assuming that the difference in lag phase is caused by a difference in the distance between the
promoter and the corresponding gene, and taking into account the average rates of transcription and translation, the locations
of the polymerase gene and the lysozyme gene on the phage genome were estimated. The distance between the promoter and the
distal end of the lysozyme gene corresponds to an RNA of the molecular weight 2.2 x 10 6 . The polymerase gene is closer to the promoter. Transcription of T7 DNA by Escherichia coli RNA polymerase in vitro does indeed result in a polycistronic messenger RNA containing the information for synthesis of both RNA polymerase and lysozyme.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)45904-8</identifier><identifier>PMID: 4942323</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Adenosine Monophosphate - metabolism ; Carbon Isotopes ; Cell-Free System ; Chromosomes ; Coliphages ; Depression, Chemical ; DNA, Bacterial - metabolism ; DNA, Viral - metabolism ; Electrophoresis, Disc ; Escherichia coli - cytology ; Escherichia coli - drug effects ; Escherichia coli - enzymology ; Escherichia coli - metabolism ; Genes ; Genetic Code ; Kinetics ; Magnesium - pharmacology ; Metabolism - drug effects ; Muramidase - biosynthesis ; Mutation ; Osmolar Concentration ; Ribosomes - metabolism ; Rifampin - pharmacology ; RNA Nucleotidyltransferases - biosynthesis ; RNA, Messenger - biosynthesis ; Stimulation, Chemical ; Templates, Genetic ; Time Factors ; Uncoupling Agents - pharmacology ; Uracil Nucleotides - metabolism</subject><ispartof>The Journal of biological chemistry, 1971-11, Vol.246 (22), p.6707-6712</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-93874e5a2e0f406d55db1330a089ac7ac92edbf3398db332e1c2963bf63c76f03</citedby><cites>FETCH-LOGICAL-c379t-93874e5a2e0f406d55db1330a089ac7ac92edbf3398db332e1c2963bf63c76f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4942323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schweiger, M</creatorcontrib><creatorcontrib>Herrlich, P</creatorcontrib><creatorcontrib>Millette, R L</creatorcontrib><title>Gene Expression in Vitro from Deoxyribonucleic Acid of Bacteriophage T7</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The in vitro synthesis of lysozyme and RNA polymerase directed by DNA of bacteriophage T7 starts with different lag periods after addition
of the template. The difference in lag period before appearance of enzyme could be attributed to a difference in the time
needed for transcription. Assuming that the difference in lag phase is caused by a difference in the distance between the
promoter and the corresponding gene, and taking into account the average rates of transcription and translation, the locations
of the polymerase gene and the lysozyme gene on the phage genome were estimated. The distance between the promoter and the
distal end of the lysozyme gene corresponds to an RNA of the molecular weight 2.2 x 10 6 . The polymerase gene is closer to the promoter. Transcription of T7 DNA by Escherichia coli RNA polymerase in vitro does indeed result in a polycistronic messenger RNA containing the information for synthesis of both RNA polymerase and lysozyme.</description><subject>Adenosine Monophosphate - metabolism</subject><subject>Carbon Isotopes</subject><subject>Cell-Free System</subject><subject>Chromosomes</subject><subject>Coliphages</subject><subject>Depression, Chemical</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Viral - metabolism</subject><subject>Electrophoresis, Disc</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - metabolism</subject><subject>Genes</subject><subject>Genetic Code</subject><subject>Kinetics</subject><subject>Magnesium - pharmacology</subject><subject>Metabolism - drug effects</subject><subject>Muramidase - biosynthesis</subject><subject>Mutation</subject><subject>Osmolar Concentration</subject><subject>Ribosomes - metabolism</subject><subject>Rifampin - pharmacology</subject><subject>RNA Nucleotidyltransferases - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stimulation, Chemical</subject><subject>Templates, Genetic</subject><subject>Time Factors</subject><subject>Uncoupling Agents - pharmacology</subject><subject>Uracil Nucleotides - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKw0AUhgdRaq0-QmFAEF1E55LbLLXWKhRcWMXdMJmcNCNJps4k2L696YWezVn8338OfAiNKbmnhMYPH4QwGggWpbdU3IWRIGGQnqAhJSkPeES_T9HwiJyjC-9_SD-hoAM0CEXIOONDNJtBA3i6Xjnw3tgGmwZ_mdZZXDhb42ew640zmW06XYHR-FGbHNsCPyndgjN2Vaol4EVyic4KVXm4OuwR-nyZLiavwfx99jZ5nAeaJ6INBE-TECLFgBQhifMoyjPKOVEkFUonSgsGeVZwLtI845wB1UzEPCtirpO4IHyEbvZ3V87-duBbWRuvoapUA7bzMqVUMEFoD0Z7UDvrvYNCrpypldtISuRWoNwJlFs7kgq5EyjTvjc-POiyGvJj62Csz6_3eWmW5Z9xIDNjdQm1ZGEsGZNxQhL-Dwt2dn4</recordid><startdate>19711125</startdate><enddate>19711125</enddate><creator>Schweiger, M</creator><creator>Herrlich, P</creator><creator>Millette, R L</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19711125</creationdate><title>Gene Expression in Vitro from Deoxyribonucleic Acid of Bacteriophage T7</title><author>Schweiger, M ; Herrlich, P ; Millette, R L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-93874e5a2e0f406d55db1330a089ac7ac92edbf3398db332e1c2963bf63c76f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Adenosine Monophosphate - metabolism</topic><topic>Carbon Isotopes</topic><topic>Cell-Free System</topic><topic>Chromosomes</topic><topic>Coliphages</topic><topic>Depression, Chemical</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Viral - metabolism</topic><topic>Electrophoresis, Disc</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - metabolism</topic><topic>Genes</topic><topic>Genetic Code</topic><topic>Kinetics</topic><topic>Magnesium - pharmacology</topic><topic>Metabolism - drug effects</topic><topic>Muramidase - biosynthesis</topic><topic>Mutation</topic><topic>Osmolar Concentration</topic><topic>Ribosomes - metabolism</topic><topic>Rifampin - pharmacology</topic><topic>RNA Nucleotidyltransferases - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Stimulation, Chemical</topic><topic>Templates, Genetic</topic><topic>Time Factors</topic><topic>Uncoupling Agents - pharmacology</topic><topic>Uracil Nucleotides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schweiger, M</creatorcontrib><creatorcontrib>Herrlich, P</creatorcontrib><creatorcontrib>Millette, R L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schweiger, M</au><au>Herrlich, P</au><au>Millette, R L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene Expression in Vitro from Deoxyribonucleic Acid of Bacteriophage T7</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1971-11-25</date><risdate>1971</risdate><volume>246</volume><issue>22</issue><spage>6707</spage><epage>6712</epage><pages>6707-6712</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The in vitro synthesis of lysozyme and RNA polymerase directed by DNA of bacteriophage T7 starts with different lag periods after addition
of the template. The difference in lag period before appearance of enzyme could be attributed to a difference in the time
needed for transcription. Assuming that the difference in lag phase is caused by a difference in the distance between the
promoter and the corresponding gene, and taking into account the average rates of transcription and translation, the locations
of the polymerase gene and the lysozyme gene on the phage genome were estimated. The distance between the promoter and the
distal end of the lysozyme gene corresponds to an RNA of the molecular weight 2.2 x 10 6 . The polymerase gene is closer to the promoter. Transcription of T7 DNA by Escherichia coli RNA polymerase in vitro does indeed result in a polycistronic messenger RNA containing the information for synthesis of both RNA polymerase and lysozyme.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>4942323</pmid><doi>10.1016/S0021-9258(19)45904-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenosine Monophosphate - metabolism Carbon Isotopes Cell-Free System Chromosomes Coliphages Depression, Chemical DNA, Bacterial - metabolism DNA, Viral - metabolism Electrophoresis, Disc Escherichia coli - cytology Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - metabolism Genes Genetic Code Kinetics Magnesium - pharmacology Metabolism - drug effects Muramidase - biosynthesis Mutation Osmolar Concentration Ribosomes - metabolism Rifampin - pharmacology RNA Nucleotidyltransferases - biosynthesis RNA, Messenger - biosynthesis Stimulation, Chemical Templates, Genetic Time Factors Uncoupling Agents - pharmacology Uracil Nucleotides - metabolism |
| title | Gene Expression in Vitro from Deoxyribonucleic Acid of Bacteriophage T7 |
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