Lysis of mouse macrophages, fibroblasts, and epidermal cells by epidermal alloantigen-specific cytotoxic T lymphocytes: Effect of culture and inflammatory agents on Epa-1 expression
The expression of Epa-1, a tissue-restricted non-major histocompatibility complex (MHC) alloantigen, on CBA epidermal cells (EC), fibroblasts (FB), and macrophages (Mφ) was investigated using bulk-cultured and clonally-derived anti-Epa-1 cytotoxic T lymphocytes (CTL). Epa-1 was readily detected on f...
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| Veröffentlicht in: | Cellular immunology 1984-09, Vol.87 (2), p.553-565 |
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| creator | Burlingham, William J. Snider, Mary Ellen Tyler, John D. Steinmuller, David |
| description | The expression of Epa-1, a tissue-restricted non-major histocompatibility complex (MHC) alloantigen, on CBA epidermal cells (EC), fibroblasts (FB), and macrophages (Mφ) was investigated using bulk-cultured and clonally-derived anti-Epa-1 cytotoxic T lymphocytes (CTL). Epa-1 was readily detected on freshly trypsinized and 24-hr-cultured EC, and on skin FB cultured for 1–3 weeks. In contrast, fresh peritoneal (PE) Mφ were specifically resistant to Epa-1 CTL but became susceptible after 12–24 hr in culture. Epa-1 expression by PE Mφ also could be induced
in vivo by Mφ-activating agents such as concanavalin A or Bacillus Calmette-Guérin (BCG), but not by the sterile inflammatory agents peptone broth or thioglycolate, suggesting a correlation between Epa-1 phenotype and Mφ activation. From this and from parallel studies of spleen cell Mφ it is concluded that Epa-1 may be a strain-specific marker for activated Mφ in the mouse, as well as an inducible histocompatibility antigen
in vivo. |
| doi_str_mv | 10.1016/0008-8749(84)90024-8 |
| format | Article |
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in vivo by Mφ-activating agents such as concanavalin A or Bacillus Calmette-Guérin (BCG), but not by the sterile inflammatory agents peptone broth or thioglycolate, suggesting a correlation between Epa-1 phenotype and Mφ activation. From this and from parallel studies of spleen cell Mφ it is concluded that Epa-1 may be a strain-specific marker for activated Mφ in the mouse, as well as an inducible histocompatibility antigen
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in vivo by Mφ-activating agents such as concanavalin A or Bacillus Calmette-Guérin (BCG), but not by the sterile inflammatory agents peptone broth or thioglycolate, suggesting a correlation between Epa-1 phenotype and Mφ activation. From this and from parallel studies of spleen cell Mφ it is concluded that Epa-1 may be a strain-specific marker for activated Mφ in the mouse, as well as an inducible histocompatibility antigen
in vivo.</description><subject>Animals</subject><subject>Antigens, Surface - immunology</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - immunology</subject><subject>Bone Marrow Cells</subject><subject>Cells, Cultured</subject><subject>Concanavalin A - pharmacology</subject><subject>Cytotoxicity, Immunologic</subject><subject>Epidermis - immunology</subject><subject>Female</subject><subject>Fibroblasts - immunology</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Immunity, Cellular</topic><topic>Macrophages - immunology</topic><topic>Mice</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burlingham, William J.</creatorcontrib><creatorcontrib>Snider, Mary Ellen</creatorcontrib><creatorcontrib>Tyler, John D.</creatorcontrib><creatorcontrib>Steinmuller, David</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burlingham, William J.</au><au>Snider, Mary Ellen</au><au>Tyler, John D.</au><au>Steinmuller, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lysis of mouse macrophages, fibroblasts, and epidermal cells by epidermal alloantigen-specific cytotoxic T lymphocytes: Effect of culture and inflammatory agents on Epa-1 expression</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1984-09</date><risdate>1984</risdate><volume>87</volume><issue>2</issue><spage>553</spage><epage>565</epage><pages>553-565</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><coden>CLIMB8</coden><abstract>The expression of Epa-1, a tissue-restricted non-major histocompatibility complex (MHC) alloantigen, on CBA epidermal cells (EC), fibroblasts (FB), and macrophages (Mφ) was investigated using bulk-cultured and clonally-derived anti-Epa-1 cytotoxic T lymphocytes (CTL). Epa-1 was readily detected on freshly trypsinized and 24-hr-cultured EC, and on skin FB cultured for 1–3 weeks. In contrast, fresh peritoneal (PE) Mφ were specifically resistant to Epa-1 CTL but became susceptible after 12–24 hr in culture. Epa-1 expression by PE Mφ also could be induced
in vivo by Mφ-activating agents such as concanavalin A or Bacillus Calmette-Guérin (BCG), but not by the sterile inflammatory agents peptone broth or thioglycolate, suggesting a correlation between Epa-1 phenotype and Mφ activation. From this and from parallel studies of spleen cell Mφ it is concluded that Epa-1 may be a strain-specific marker for activated Mφ in the mouse, as well as an inducible histocompatibility antigen
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| subjects | Animals Antigens, Surface - immunology Biological and medical sciences Bone Marrow - immunology Bone Marrow Cells Cells, Cultured Concanavalin A - pharmacology Cytotoxicity, Immunologic Epidermis - immunology Female Fibroblasts - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Immunity, Cellular Macrophages - immunology Mice T-Lymphocytes, Cytotoxic - immunology Tissue, organ and graft immunology |
| title | Lysis of mouse macrophages, fibroblasts, and epidermal cells by epidermal alloantigen-specific cytotoxic T lymphocytes: Effect of culture and inflammatory agents on Epa-1 expression |
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