Expression of protozoan antigens by hybrids between Leishmania mexicana and myeloma SP2/0

Hybrid cells were obtained between Leishmania mexicana promastigotes and mouse myeloma SP2/0 cells, and examined for expression of leishmanial antigens. A ratio of 1:10 of myeloma to T. cruzi cells was unsuccessful because of outgrowth of non-fused cells. With a ratio of 2:1 four waves of multiplica...

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Veröffentlicht in:	Journal of immunological methods 1984-08, Vol.72 (1), p.97-107
Hauptverfasser:	Tomina, Hélène, Marcipar, Alberto J., Broun, Georges B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Surface - analysis Biological and medical sciences Cell Fusion Cell Line Fundamental and applied biological sciences. Psychology Hybridomas - immunology Hybridomas - parasitology Karyotyping Leishmania - genetics Leishmania - growth & development Leishmania - immunology Leishmania mexicana antigens Life cycle. Host-agent relationship. Pathogenesis Mice Mice, Inbred BALB C myeloma hybrids Plasmacytoma - genetics Plasmacytoma - immunology Protozoa protozoon
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