Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride

Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease...

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Veröffentlicht in:	Biochemical and biophysical research communications 1984-06, Vol.121 (3), p.907-914
Hauptverfasser:	Jordano, Juan, Montero, Francisco, Palacián, Enrique
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Acetic Anhydrides Animals Centrifugation, Density Gradient Chickens DNA, Single-Stranded - blood DNA, Single-Stranded - isolation & purification Erythrocytes - analysis Hot Temperature Nucleic Acid Denaturation Nucleosomes - analysis Osmolar Concentration
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