Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride
Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-06, Vol.121 (3), p.907-914 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Jordano, Juan Montero, Francisco Palacián, Enrique |
| description | Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease S1, and elution from hydroxyapatite. In contrast, if modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of lysine-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic stregth. |
| doi_str_mv | 10.1016/0006-291X(84)90763-0 |
| format | Article |
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This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease S1, and elution from hydroxyapatite. In contrast, if modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of lysine-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic stregth.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(84)90763-0</identifier><identifier>PMID: 6743321</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetates ; Acetic Anhydrides ; Animals ; Centrifugation, Density Gradient ; Chickens ; DNA, Single-Stranded - blood ; DNA, Single-Stranded - isolation & purification ; Erythrocytes - analysis ; Hot Temperature ; Nucleic Acid Denaturation ; Nucleosomes - analysis ; Osmolar Concentration</subject><ispartof>Biochemical and biophysical research communications, 1984-06, Vol.121 (3), p.907-914</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-32ca6e314d53ef6a37978df2737b37a36e7429ee7f57ac0a0459aa614b571d053</citedby><cites>FETCH-LOGICAL-c434t-32ca6e314d53ef6a37978df2737b37a36e7429ee7f57ac0a0459aa614b571d053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(84)90763-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6743321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jordano, Juan</creatorcontrib><creatorcontrib>Montero, Francisco</creatorcontrib><creatorcontrib>Palacián, Enrique</creatorcontrib><title>Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease S1, and elution from hydroxyapatite. In contrast, if modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of lysine-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic stregth.</description><subject>Acetates</subject><subject>Acetic Anhydrides</subject><subject>Animals</subject><subject>Centrifugation, Density Gradient</subject><subject>Chickens</subject><subject>DNA, Single-Stranded - blood</subject><subject>DNA, Single-Stranded - isolation & purification</subject><subject>Erythrocytes - analysis</subject><subject>Hot Temperature</subject><subject>Nucleic Acid Denaturation</subject><subject>Nucleosomes - analysis</subject><subject>Osmolar Concentration</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1OHDEQhC0URDaENyCSTxE5TOgee-z1JRIC8iMhuCQSN8trt8HRzJjYsyDePrPsimNy6kN9VS1VMXaM8BkB1SkAqKY1eHuylJ8MaCUa2GMLBANNiyDfsMUr8pa9q_U3AKJU5oAdKC2FaHHB3EWqNfvkppRHniOvabzrqalTcWOgwC-uz3gseeDj2veUax6o8pj7Pj_NJB9ySDH5rf0pTffceZqS5268fw4lBXrP9qPrKx3t7iH79fXy5_n35urm24_zs6vGSyGnRrTeKRIoQycoKie00csQWy30SmgnFGnZGiIdO-08OJCdcU6hXHUaA3TikH3c5j6U_GdNdbJDqp763o2U19UuEVVrpPkviMIY0wLOoNyCvuRaC0X7UNLgyrNFsJsJ7KZfu-nXLqV9mcDCbPuwy1-vBgqvpl3ns_5lq9PcxmOiYqtPNHoKqZCfbMjp3w_-AiDslls</recordid><startdate>19840629</startdate><enddate>19840629</enddate><creator>Jordano, Juan</creator><creator>Montero, Francisco</creator><creator>Palacián, Enrique</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19840629</creationdate><title>Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride</title><author>Jordano, Juan ; Montero, Francisco ; Palacián, Enrique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-32ca6e314d53ef6a37978df2737b37a36e7429ee7f57ac0a0459aa614b571d053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Acetates</topic><topic>Acetic Anhydrides</topic><topic>Animals</topic><topic>Centrifugation, Density Gradient</topic><topic>Chickens</topic><topic>DNA, Single-Stranded - blood</topic><topic>DNA, Single-Stranded - isolation & purification</topic><topic>Erythrocytes - analysis</topic><topic>Hot Temperature</topic><topic>Nucleic Acid Denaturation</topic><topic>Nucleosomes - analysis</topic><topic>Osmolar Concentration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jordano, Juan</creatorcontrib><creatorcontrib>Montero, Francisco</creatorcontrib><creatorcontrib>Palacián, Enrique</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jordano, Juan</au><au>Montero, Francisco</au><au>Palacián, Enrique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-06-29</date><risdate>1984</risdate><volume>121</volume><issue>3</issue><spage>907</spage><epage>914</epage><pages>907-914</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease S1, and elution from hydroxyapatite. In contrast, if modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of lysine-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic stregth.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6743321</pmid><doi>10.1016/0006-291X(84)90763-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1984-06, Vol.121 (3), p.907-914 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetates Acetic Anhydrides Animals Centrifugation, Density Gradient Chickens DNA, Single-Stranded - blood DNA, Single-Stranded - isolation & purification Erythrocytes - analysis Hot Temperature Nucleic Acid Denaturation Nucleosomes - analysis Osmolar Concentration |
| title | Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride |
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