Structure elucidation of platelet activating factor derived from human neutrophils
Platelet activating factor (PAF) ★ ★ The abbreviation PAF is used to refer to biological activity and AGEPC will be used to describe a specific chemical entity. synthesized by human neutrophils challenged by opsonized zymosan or calcium ionophore was isolated from cells and buffer using Bligh and Dy...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-06, Vol.121 (3), p.815-825 |
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| container_end_page | 825 |
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| container_issue | 3 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 121 |
| creator | Clay, Keith L. Murphy, Robert C. Andres, Janet L. Lynch, James Henson, Peter M. |
| description | Platelet activating factor (PAF)
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The abbreviation PAF is used to refer to biological activity and AGEPC will be used to describe a specific chemical entity.
synthesized by human neutrophils challenged by opsonized zymosan or calcium ionophore was isolated from cells and buffer using Bligh and Dyer extraction following the addition of tracer amounts of tritiated-PAF. The extract was subjected to TLC separation of phospholipid classes, followed by reverse phase HPLC for molecular species separation. All fractions were measured for radioactivity, biological activity and fast atom bombardment mass spectrometry. While the radioactive tracer PAF could be separated into three molecular species, PAF biological activity eluted as a single component which was characterized as 1-O-hexadecyl-2-acetyl-glycero-3-phosphocholine. The lack of molecular species heterogeneity of PAF produced in response to stimuli implies a higher degree of control of biosynthesis than previously suspected. |
| doi_str_mv | 10.1016/0006-291X(84)90751-4 |
| format | Article |
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★
★
The abbreviation PAF is used to refer to biological activity and AGEPC will be used to describe a specific chemical entity.
synthesized by human neutrophils challenged by opsonized zymosan or calcium ionophore was isolated from cells and buffer using Bligh and Dyer extraction following the addition of tracer amounts of tritiated-PAF. The extract was subjected to TLC separation of phospholipid classes, followed by reverse phase HPLC for molecular species separation. All fractions were measured for radioactivity, biological activity and fast atom bombardment mass spectrometry. While the radioactive tracer PAF could be separated into three molecular species, PAF biological activity eluted as a single component which was characterized as 1-O-hexadecyl-2-acetyl-glycero-3-phosphocholine. The lack of molecular species heterogeneity of PAF produced in response to stimuli implies a higher degree of control of biosynthesis than previously suspected.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(84)90751-4</identifier><identifier>PMID: 6430285</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Calcimycin - pharmacology ; Chemical Phenomena ; Chemistry ; Chromatography, High Pressure Liquid ; Gas Chromatography-Mass Spectrometry ; Humans ; Mass Spectrometry ; Neutrophils - metabolism ; Platelet Activating Factor - biosynthesis ; Zymosan - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 1984-06, Vol.121 (3), p.815-825</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-1905bbfb8caa26096d8f231606409d6c50371162757afc87f3847fc3c752236f3</citedby><cites>FETCH-LOGICAL-c408t-1905bbfb8caa26096d8f231606409d6c50371162757afc87f3847fc3c752236f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(84)90751-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6430285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clay, Keith L.</creatorcontrib><creatorcontrib>Murphy, Robert C.</creatorcontrib><creatorcontrib>Andres, Janet L.</creatorcontrib><creatorcontrib>Lynch, James</creatorcontrib><creatorcontrib>Henson, Peter M.</creatorcontrib><title>Structure elucidation of platelet activating factor derived from human neutrophils</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Platelet activating factor (PAF)
★
★
The abbreviation PAF is used to refer to biological activity and AGEPC will be used to describe a specific chemical entity.
synthesized by human neutrophils challenged by opsonized zymosan or calcium ionophore was isolated from cells and buffer using Bligh and Dyer extraction following the addition of tracer amounts of tritiated-PAF. The extract was subjected to TLC separation of phospholipid classes, followed by reverse phase HPLC for molecular species separation. All fractions were measured for radioactivity, biological activity and fast atom bombardment mass spectrometry. While the radioactive tracer PAF could be separated into three molecular species, PAF biological activity eluted as a single component which was characterized as 1-O-hexadecyl-2-acetyl-glycero-3-phosphocholine. The lack of molecular species heterogeneity of PAF produced in response to stimuli implies a higher degree of control of biosynthesis than previously suspected.</description><subject>Adult</subject><subject>Calcimycin - pharmacology</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Mass Spectrometry</subject><subject>Neutrophils - metabolism</subject><subject>Platelet Activating Factor - biosynthesis</subject><subject>Zymosan - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLLDEQhYMoOj7-gUJWcl20VtLpdHpz4SK-QBB8gLuQSVc00t2Zm6QH_Pf2OINLV1XUOaeK-gg5ZnDOgMkLAJAFb9jrHyXOGqgrVogtMmPQQMEZiG0y-7Hskf2UPgAYE7LZJbtSlMBVNSOPTzmONo8RKXaj9a3JPgw0OLroTMYOMzU2--U0Ht6om_oQaYvRL7GlLoaevo-9GeiAY45h8e67dEh2nOkSHm3qAXm5vnq-vC3uH27uLv_dF1aAygVroJrP3VxZY7iERrbK8ZJJkAKaVtoKypoxyeuqNs6q2pVK1M6Wtq44L6UrD8jpeu8ihv8jpqx7nyx2nRkwjEmrVZoLmIxibbQxpBTR6UX0vYmfmoFeodQrTnrFSSuhv1FqMcVONvvHeY_tT2jDbtL_rnWcnlx6jDpZj4PF1ke0WbfB_37gC0xSg1M</recordid><startdate>19840629</startdate><enddate>19840629</enddate><creator>Clay, Keith L.</creator><creator>Murphy, Robert C.</creator><creator>Andres, Janet L.</creator><creator>Lynch, James</creator><creator>Henson, Peter M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19840629</creationdate><title>Structure elucidation of platelet activating factor derived from human neutrophils</title><author>Clay, Keith L. ; Murphy, Robert C. ; Andres, Janet L. ; Lynch, James ; Henson, Peter M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-1905bbfb8caa26096d8f231606409d6c50371162757afc87f3847fc3c752236f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adult</topic><topic>Calcimycin - pharmacology</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Mass Spectrometry</topic><topic>Neutrophils - metabolism</topic><topic>Platelet Activating Factor - biosynthesis</topic><topic>Zymosan - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clay, Keith L.</creatorcontrib><creatorcontrib>Murphy, Robert C.</creatorcontrib><creatorcontrib>Andres, Janet L.</creatorcontrib><creatorcontrib>Lynch, James</creatorcontrib><creatorcontrib>Henson, Peter M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clay, Keith L.</au><au>Murphy, Robert C.</au><au>Andres, Janet L.</au><au>Lynch, James</au><au>Henson, Peter M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure elucidation of platelet activating factor derived from human neutrophils</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-06-29</date><risdate>1984</risdate><volume>121</volume><issue>3</issue><spage>815</spage><epage>825</epage><pages>815-825</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Platelet activating factor (PAF)
★
★
The abbreviation PAF is used to refer to biological activity and AGEPC will be used to describe a specific chemical entity.
synthesized by human neutrophils challenged by opsonized zymosan or calcium ionophore was isolated from cells and buffer using Bligh and Dyer extraction following the addition of tracer amounts of tritiated-PAF. The extract was subjected to TLC separation of phospholipid classes, followed by reverse phase HPLC for molecular species separation. All fractions were measured for radioactivity, biological activity and fast atom bombardment mass spectrometry. While the radioactive tracer PAF could be separated into three molecular species, PAF biological activity eluted as a single component which was characterized as 1-O-hexadecyl-2-acetyl-glycero-3-phosphocholine. The lack of molecular species heterogeneity of PAF produced in response to stimuli implies a higher degree of control of biosynthesis than previously suspected.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6430285</pmid><doi>10.1016/0006-291X(84)90751-4</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1984-06, Vol.121 (3), p.815-825 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81162240 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Calcimycin - pharmacology Chemical Phenomena Chemistry Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Humans Mass Spectrometry Neutrophils - metabolism Platelet Activating Factor - biosynthesis Zymosan - pharmacology |
| title | Structure elucidation of platelet activating factor derived from human neutrophils |
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