Cryobiology of Neurospora crassa: I. Freeze response of Neurospora crassa conidia
Neurospora crassa conidia were frozen and thawed in water suspensions at various rates and with different minimum temperatures. Colony counts of the experimental conidia were compared with those of controls, which were taken as 100% survival. The data revealed that (1) survivals were near 100% after...
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| Veröffentlicht in: | Cryobiology 1971-08, Vol.8 (4), p.323-327 |
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| container_title | Cryobiology |
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| creator | Barnhart, E.E. Terry, Claude E. |
| description | Neurospora crassa conidia were frozen and thawed in water suspensions at various rates and with different minimum temperatures. Colony counts of the experimental conidia were compared with those of controls, which were taken as 100% survival. The data revealed that (1) survivals were near 100% after fast thaw (400 °/min) regardless of the freeze rate, (2) percentage of survival was inversely related to freeze rate when combined with slow thaw, (3) slow thaw (0.5 °/min) was damaging, and (3) the rates of freeze-thaw affected the system only in the −5 to −20 ° interval. The damaging freeze conditions were those which favor ice crystal growth. It is suggested that rupture of the membrane by ice crystals seems to be the plausible mechanism of damage in freezing and thawing
N. crassa conidia. |
| doi_str_mv | 10.1016/0011-2240(71)90125-8 |
| format | Article |
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N. crassa conidia.</description><subject>Crystallization</subject><subject>Freezing</subject><subject>Ice</subject><subject>Neurospora</subject><subject>Spores</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEFLAzEQRoMotVb_gcKeRA-rM0k2u-tBkGK1UBRBzyGbTCWybWrSCvXX27XFk6c5vMfw8Rg7RbhCQHUNgJhzLuGixMsakBd5tcf6CDXkXNR8n_X_lEN2lNIHAKhSyB7rFchBcdlnL8O4Do0PbXhfZ2GaPdEqhrQI0WQ2mpTMTTa-ykaR6JuySBsyT_SvmNkw986bY3YwNW2ik90dsLfR_evwMZ88P4yHd5OcsFTLvBAga5KFBG5rRc5VUDamkU4Y4Rpb1ZuxTekkCrKlksYYNUVTKkDpDK-FGLDz7d9FDJ8rSks988lS25o5hVXSFWJRSNWJZztx1czI6UX0MxPXetdgw2-3nDZrvzxFnaynuSXnI9mldsFrBN01111Q3QXVJerf5roSP_-Mcfc</recordid><startdate>197108</startdate><enddate>197108</enddate><creator>Barnhart, E.E.</creator><creator>Terry, Claude E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197108</creationdate><title>Cryobiology of Neurospora crassa: I. Freeze response of Neurospora crassa conidia</title><author>Barnhart, E.E. ; Terry, Claude E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e176t-53049e45402c96edd807bab4d3a3dbc89000b7d413ec764aaa6f1a76014da2933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Crystallization</topic><topic>Freezing</topic><topic>Ice</topic><topic>Neurospora</topic><topic>Spores</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barnhart, E.E.</creatorcontrib><creatorcontrib>Terry, Claude E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barnhart, E.E.</au><au>Terry, Claude E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryobiology of Neurospora crassa: I. Freeze response of Neurospora crassa conidia</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>1971-08</date><risdate>1971</risdate><volume>8</volume><issue>4</issue><spage>323</spage><epage>327</epage><pages>323-327</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>Neurospora crassa conidia were frozen and thawed in water suspensions at various rates and with different minimum temperatures. Colony counts of the experimental conidia were compared with those of controls, which were taken as 100% survival. The data revealed that (1) survivals were near 100% after fast thaw (400 °/min) regardless of the freeze rate, (2) percentage of survival was inversely related to freeze rate when combined with slow thaw, (3) slow thaw (0.5 °/min) was damaging, and (3) the rates of freeze-thaw affected the system only in the −5 to −20 ° interval. The damaging freeze conditions were those which favor ice crystal growth. It is suggested that rupture of the membrane by ice crystals seems to be the plausible mechanism of damage in freezing and thawing
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| ispartof | Cryobiology, 1971-08, Vol.8 (4), p.323-327 |
| issn | 0011-2240 1090-2392 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Crystallization Freezing Ice Neurospora Spores Temperature Time Factors |
| title | Cryobiology of Neurospora crassa: I. Freeze response of Neurospora crassa conidia |
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