Quantitation of xanthurenic acid in rabbit serum using high performance liquid chromatography
Xanthurenic acid (XA) has been quantified in the serum of normal and vitamin B6-deficient rabbits using high performance liquid chromatography. The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of d...
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| Veröffentlicht in: | The American journal of clinical nutrition 1984-07, Vol.40 (1), p.159-167 |
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| container_title | The American journal of clinical nutrition |
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| creator | Williams, S A Monti, J A Boots, L R Cornwell, P E |
| description | Xanthurenic acid (XA) has been quantified in the serum of normal and vitamin B6-deficient rabbits using high performance liquid chromatography. The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of dilutions of standard XA (3.9 to 1000 ng) ranged from 45.5% at the lower limit of the curve to 10.9% at the higher range of the curve. The minimum detectable level was 3.9 ng/ml. Serum samples spiked with reference XA exhibited a parallel dose response. The percentage recovery of XA from serum samples was 80.8%. The procedure, which requires 1 to 2 ml of serum, is sensitive and may be a useful tool for assessing B6 nutritional parameters as well as the physiological role of XA. It offers advantages over urinary procedures because it is more sensitive, more specific, and allows the study of blood levels of XA. |
| doi_str_mv | 10.1093/ajcn/40.1.159 |
| format | Article |
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The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of dilutions of standard XA (3.9 to 1000 ng) ranged from 45.5% at the lower limit of the curve to 10.9% at the higher range of the curve. The minimum detectable level was 3.9 ng/ml. Serum samples spiked with reference XA exhibited a parallel dose response. The percentage recovery of XA from serum samples was 80.8%. The procedure, which requires 1 to 2 ml of serum, is sensitive and may be a useful tool for assessing B6 nutritional parameters as well as the physiological role of XA. 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The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of dilutions of standard XA (3.9 to 1000 ng) ranged from 45.5% at the lower limit of the curve to 10.9% at the higher range of the curve. The minimum detectable level was 3.9 ng/ml. Serum samples spiked with reference XA exhibited a parallel dose response. The percentage recovery of XA from serum samples was 80.8%. The procedure, which requires 1 to 2 ml of serum, is sensitive and may be a useful tool for assessing B6 nutritional parameters as well as the physiological role of XA. It offers advantages over urinary procedures because it is more sensitive, more specific, and allows the study of blood levels of XA.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Female</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>High performance liquid chromatography</subject><subject>Kynurenic Acid - analogs & derivatives</subject><subject>Kynurenic Acid - blood</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>pyridoxine</subject><subject>rabbit serum</subject><subject>Rabbits</subject><subject>Tryptophan - metabolism</subject><subject>Vitamin B 6 Deficiency - blood</subject><subject>vitamin B6 deficiency</subject><subject>Xanthurenates</subject><subject>xanthurenic acid</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E1rFTEUgOEgSr1Wly7FLMTdtDn5mmQppX5AQUS7lJBkkjspM5PbZEbsvzflXrpzFQ7n4RBehN4CuQCi2aW988slb8MFCP0M7UAz1TFK-udoRwihnQYpXqJXtd4RApQreYbOZM9Bcb1Dv39sdlnTateUF5wj_tvGcSthSR5bnwacFlysc2nFNZRtxltNyx6PaT_iQygxl9kuPuAp3W9N-7Hk2a55X-xhfHiNXkQ71fDm9J6j28_Xv66-djffv3y7-nTTeU7V2lEOTAoieAStqBMCvBY9F9z1fHCSuOgCi1JFxcDZPkQKnEXiQfXAwVF2jj4e7x5Kvt9CXc2cqg_TZJeQt2oUgGCC6wa7I_Ql11pCNIeSZlseDBDzmNM85jS8DablbP7d6fDm5jA86VO_tv9w2tvq7RRLa5HqE9NEEsZZY--PLNps7L40cvuTEmCECkkkF030RxFapj8pFFN9Ci3skErwqxly-s8X_wEzEpo5</recordid><startdate>198407</startdate><enddate>198407</enddate><creator>Williams, S A</creator><creator>Monti, J A</creator><creator>Boots, L R</creator><creator>Cornwell, P E</creator><general>Elsevier Inc</general><general>American Society for Clinical Nutrition</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198407</creationdate><title>Quantitation of xanthurenic acid in rabbit serum using high performance liquid chromatography</title><author>Williams, S A ; Monti, J A ; Boots, L R ; Cornwell, P E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-241365054f1982b551c957454b74db60bfbe3f68f831ba7ef2143f0c187141b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Female</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>High performance liquid chromatography</topic><topic>Kynurenic Acid - analogs & derivatives</topic><topic>Kynurenic Acid - blood</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>pyridoxine</topic><topic>rabbit serum</topic><topic>Rabbits</topic><topic>Tryptophan - metabolism</topic><topic>Vitamin B 6 Deficiency - blood</topic><topic>vitamin B6 deficiency</topic><topic>Xanthurenates</topic><topic>xanthurenic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williams, S A</creatorcontrib><creatorcontrib>Monti, J A</creatorcontrib><creatorcontrib>Boots, L R</creatorcontrib><creatorcontrib>Cornwell, P E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williams, S A</au><au>Monti, J A</au><au>Boots, L R</au><au>Cornwell, P E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of xanthurenic acid in rabbit serum using high performance liquid chromatography</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>1984-07</date><risdate>1984</risdate><volume>40</volume><issue>1</issue><spage>159</spage><epage>167</epage><pages>159-167</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>Xanthurenic acid (XA) has been quantified in the serum of normal and vitamin B6-deficient rabbits using high performance liquid chromatography. The concentration of XA in the serum of normal and B6-deficient rabbits was 141 and 2275 ng/ml, respectively. The coefficient of variation for a series of dilutions of standard XA (3.9 to 1000 ng) ranged from 45.5% at the lower limit of the curve to 10.9% at the higher range of the curve. The minimum detectable level was 3.9 ng/ml. Serum samples spiked with reference XA exhibited a parallel dose response. The percentage recovery of XA from serum samples was 80.8%. The procedure, which requires 1 to 2 ml of serum, is sensitive and may be a useful tool for assessing B6 nutritional parameters as well as the physiological role of XA. It offers advantages over urinary procedures because it is more sensitive, more specific, and allows the study of blood levels of XA.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>6741849</pmid><doi>10.1093/ajcn/40.1.159</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The American journal of clinical nutrition, 1984-07, Vol.40 (1), p.159-167 |
| issn | 0002-9165 1938-3207 |
| language | eng |
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| subjects | Animals Biological and medical sciences Chromatography, High Pressure Liquid Female Gas Chromatography-Mass Spectrometry High performance liquid chromatography Kynurenic Acid - analogs & derivatives Kynurenic Acid - blood Medical sciences Metabolic diseases Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) pyridoxine rabbit serum Rabbits Tryptophan - metabolism Vitamin B 6 Deficiency - blood vitamin B6 deficiency Xanthurenates xanthurenic acid |
| title | Quantitation of xanthurenic acid in rabbit serum using high performance liquid chromatography |
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