use of the latex test for the detection of distant serological relationships among plant viruses
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Virusserologie, Braunschweig, W. Germany To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres....
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| Veröffentlicht in: | Journal of general virology 1971-07, Vol.12 (1), p.25-32 |
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| container_title | Journal of general virology |
| container_volume | 12 |
| creator | Bercks, R Querfurth, G |
| description | Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Virusserologie, Braunschweig, W. Germany
To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres. In tests with latex coated with elongated viruses of the potato virus X group and with isometric viruses of the turnip yellow mosaic virus group the homologous titres of antisera were always much higher than in slide precipitin or agar diffusion tests. Usually, the heterologous titres were also increased and more heterologous reactions were found, especially when the pure -globulin fraction was used instead of unfractionated sera, even though this -globulin fraction contained relatively few antibodies.
With both groups of viruses, the results corroborate previous observations made using conventional serological methods. In addition, turnip yellow mosaic virus was shown for the first time to be distantly serologically related to Andean potato latent and belladonna mottle viruses. The latex test may offer improved opportunities to detect distant serological relationships among members of other groups of viruses.
Received 27 October 1970;
accepted 12 March 1971. |
| doi_str_mv | 10.1099/0022-1317-12-1-25 |
| format | Article |
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To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres. In tests with latex coated with elongated viruses of the potato virus X group and with isometric viruses of the turnip yellow mosaic virus group the homologous titres of antisera were always much higher than in slide precipitin or agar diffusion tests. Usually, the heterologous titres were also increased and more heterologous reactions were found, especially when the pure -globulin fraction was used instead of unfractionated sera, even though this -globulin fraction contained relatively few antibodies.
With both groups of viruses, the results corroborate previous observations made using conventional serological methods. In addition, turnip yellow mosaic virus was shown for the first time to be distantly serologically related to Andean potato latent and belladonna mottle viruses. The latex test may offer improved opportunities to detect distant serological relationships among members of other groups of viruses.
Received 27 October 1970;
accepted 12 March 1971.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-12-1-25</identifier><identifier>PMID: 4106809</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Agar ; Ammonium Sulfate ; Antigens - analysis ; Cross Reactions ; Electrophoresis ; Evaluation Studies as Topic ; gamma-Globulins ; Immune Sera ; Immunodiffusion ; Latex Fixation Tests ; Methods ; plant health ; plant viruses ; Plant Viruses - classification ; Plant Viruses - immunology ; Precipitin Tests</subject><ispartof>Journal of general virology, 1971-07, Vol.12 (1), p.25-32</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-d412117537052f98308d634bf6828e46d2ccc3da81bad7de215af74a76fc941d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3744,3745,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4106809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bercks, R</creatorcontrib><creatorcontrib>Querfurth, G</creatorcontrib><title>use of the latex test for the detection of distant serological relationships among plant viruses</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Virusserologie, Braunschweig, W. Germany
To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres. In tests with latex coated with elongated viruses of the potato virus X group and with isometric viruses of the turnip yellow mosaic virus group the homologous titres of antisera were always much higher than in slide precipitin or agar diffusion tests. Usually, the heterologous titres were also increased and more heterologous reactions were found, especially when the pure -globulin fraction was used instead of unfractionated sera, even though this -globulin fraction contained relatively few antibodies.
With both groups of viruses, the results corroborate previous observations made using conventional serological methods. In addition, turnip yellow mosaic virus was shown for the first time to be distantly serologically related to Andean potato latent and belladonna mottle viruses. The latex test may offer improved opportunities to detect distant serological relationships among members of other groups of viruses.
Received 27 October 1970;
accepted 12 March 1971.</description><subject>Agar</subject><subject>Ammonium Sulfate</subject><subject>Antigens - analysis</subject><subject>Cross Reactions</subject><subject>Electrophoresis</subject><subject>Evaluation Studies as Topic</subject><subject>gamma-Globulins</subject><subject>Immune Sera</subject><subject>Immunodiffusion</subject><subject>Latex Fixation Tests</subject><subject>Methods</subject><subject>plant health</subject><subject>plant viruses</subject><subject>Plant Viruses - classification</subject><subject>Plant Viruses - immunology</subject><subject>Precipitin Tests</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUMluFDEQtRAoTAIfwAHhE7cGl5du9xFFgSBF4gA5G49d7jHqHg-2h-XvcTOj5PSkektVPUJeAXsHbBzfM8Z5BwKGDhp2XD0hG5C96nhjn5LNA_-cXJbygzGQUg0X5EIC6zUbN-T7sSBNgdYd0tlW_EMrlkpDyv9HHiu6GtN-1fhYqt1XWjCnOU3R2ZlmbK7Gl108FGqXtJ_oYV5Vv2Ju2eUFeRbsXPDlGa_I_cebb9e33d2XT5-vP9x1Toyydl4CBxiUGJjiYdSCad8LuQ295hpl77lzTnirYWv94JGDsmGQduiDGyV4cUXennIPOf08th_MEovDud2C6ViMBhCKKd2EcBK6nErJGMwhx8XmvwaYWVs1a2tmbc1AQ8NV87w-hx-3C_oHx7nGx-W7OO1-x4xmwv0S24ZtTKY18Rj05iQMNhk75VjM_VfOQLB2Hle6F_8A6-yJ8A</recordid><startdate>197107</startdate><enddate>197107</enddate><creator>Bercks, R</creator><creator>Querfurth, G</creator><general>Soc General Microbiol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197107</creationdate><title>use of the latex test for the detection of distant serological relationships among plant viruses</title><author>Bercks, R ; Querfurth, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-d412117537052f98308d634bf6828e46d2ccc3da81bad7de215af74a76fc941d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Agar</topic><topic>Ammonium Sulfate</topic><topic>Antigens - analysis</topic><topic>Cross Reactions</topic><topic>Electrophoresis</topic><topic>Evaluation Studies as Topic</topic><topic>gamma-Globulins</topic><topic>Immune Sera</topic><topic>Immunodiffusion</topic><topic>Latex Fixation Tests</topic><topic>Methods</topic><topic>plant health</topic><topic>plant viruses</topic><topic>Plant Viruses - classification</topic><topic>Plant Viruses - immunology</topic><topic>Precipitin Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bercks, R</creatorcontrib><creatorcontrib>Querfurth, G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bercks, R</au><au>Querfurth, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>use of the latex test for the detection of distant serological relationships among plant viruses</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1971-07</date><risdate>1971</risdate><volume>12</volume><issue>1</issue><spage>25</spage><epage>32</epage><pages>25-32</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Virusserologie, Braunschweig, W. Germany
To evaluate the latex test for detecting distant serological relationships among plant viruses, latex coated first with antibody and then with virus was used to determine antiserum titres. In tests with latex coated with elongated viruses of the potato virus X group and with isometric viruses of the turnip yellow mosaic virus group the homologous titres of antisera were always much higher than in slide precipitin or agar diffusion tests. Usually, the heterologous titres were also increased and more heterologous reactions were found, especially when the pure -globulin fraction was used instead of unfractionated sera, even though this -globulin fraction contained relatively few antibodies.
With both groups of viruses, the results corroborate previous observations made using conventional serological methods. In addition, turnip yellow mosaic virus was shown for the first time to be distantly serologically related to Andean potato latent and belladonna mottle viruses. The latex test may offer improved opportunities to detect distant serological relationships among members of other groups of viruses.
Received 27 October 1970;
accepted 12 March 1971.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>4106809</pmid><doi>10.1099/0022-1317-12-1-25</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1971-07, Vol.12 (1), p.25-32 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81135058 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agar Ammonium Sulfate Antigens - analysis Cross Reactions Electrophoresis Evaluation Studies as Topic gamma-Globulins Immune Sera Immunodiffusion Latex Fixation Tests Methods plant health plant viruses Plant Viruses - classification Plant Viruses - immunology Precipitin Tests |
| title | use of the latex test for the detection of distant serological relationships among plant viruses |
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