Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells
Synthetic hydroxyapatite (HA) crystals in 1% serum stimulated 3H thymidine uptake into quiescent canine synovial fibroblasts and human foreskin fibroblast cultures, as did 10% serum. The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2—3 hours as compared wi...
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| Veröffentlicht in: | Arthritis and rheumatism 1984-06, Vol.27 (6), p.668-674 |
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| creator | Cheung, Herman S. Story, Michael T. Mccarty, Daniel J. |
| description | Synthetic hydroxyapatite (HA) crystals in 1% serum stimulated 3H thymidine uptake into quiescent canine synovial fibroblasts and human foreskin fibroblast cultures, as did 10% serum. The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2—3 hours as compared with the effects produced by 10% serum. Stimulation of 3H thymidine uptake was proportional to the serum concentration used. HA crystals (50 μg/ml) stimulated nuclide uptake at each serum concentration used. 3H thymidine uptake was also proportional to the dose of HA or calcium pyrophosphate dihydrate crystals, although larger doses of the latter crystal were required to produce equivalent effects. Not all particulates were effective mitogenic agents. Latex beads and diamond crystals had no effect. Monosodium urate crystals modestly stimulated and calcium urate crystals markedly stimulated nuclide uptake. The more complex crystals found in a naturally occurring condition (calcinosis) were as mitogenic as the pure synthetic HA. The synovial cell hyperplasia sometimes associated with crystals might be explained in part by their mitogenic activity. |
| doi_str_mv | 10.1002/art.1780270610 |
| format | Article |
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The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2—3 hours as compared with the effects produced by 10% serum. Stimulation of 3H thymidine uptake was proportional to the serum concentration used. HA crystals (50 μg/ml) stimulated nuclide uptake at each serum concentration used. 3H thymidine uptake was also proportional to the dose of HA or calcium pyrophosphate dihydrate crystals, although larger doses of the latter crystal were required to produce equivalent effects. Not all particulates were effective mitogenic agents. Latex beads and diamond crystals had no effect. Monosodium urate crystals modestly stimulated and calcium urate crystals markedly stimulated nuclide uptake. The more complex crystals found in a naturally occurring condition (calcinosis) were as mitogenic as the pure synthetic HA. The synovial cell hyperplasia sometimes associated with crystals might be explained in part by their mitogenic activity.</description><identifier>ISSN: 0004-3591</identifier><identifier>EISSN: 1529-0131</identifier><identifier>DOI: 10.1002/art.1780270610</identifier><identifier>PMID: 6329235</identifier><identifier>CODEN: ARHEAW</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Biological and medical sciences ; Calcinosis - metabolism ; Calcium Pyrophosphate - pharmacology ; Cells, Cultured ; Crystallization ; Diphosphates - pharmacology ; Dogs ; Dose-Response Relationship, Drug ; Durapatite ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Humans ; Hydroxyapatites - pharmacology ; Medical sciences ; Metabolic diseases ; Metals (hemochromatosis...) ; Mitogens ; Other metabolic disorders ; Thymidine - metabolism ; Uric Acid - pharmacology</subject><ispartof>Arthritis and rheumatism, 1984-06, Vol.27 (6), p.668-674</ispartof><rights>Copyright © 1984 American College of Rheumatology</rights><rights>1985 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4640-7304ceb28b4c2b38abdbbfbf2d1f07dcba016c89cae6993b4760ca4aeb28e8633</citedby><cites>FETCH-LOGICAL-c4640-7304ceb28b4c2b38abdbbfbf2d1f07dcba016c89cae6993b4760ca4aeb28e8633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fart.1780270610$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fart.1780270610$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8876693$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6329235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheung, Herman S.</creatorcontrib><creatorcontrib>Story, Michael T.</creatorcontrib><creatorcontrib>Mccarty, Daniel J.</creatorcontrib><title>Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells</title><title>Arthritis and rheumatism</title><addtitle>Arthritis Rheum</addtitle><description>Synthetic hydroxyapatite (HA) crystals in 1% serum stimulated 3H thymidine uptake into quiescent canine synovial fibroblasts and human foreskin fibroblast cultures, as did 10% serum. The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2—3 hours as compared with the effects produced by 10% serum. Stimulation of 3H thymidine uptake was proportional to the serum concentration used. HA crystals (50 μg/ml) stimulated nuclide uptake at each serum concentration used. 3H thymidine uptake was also proportional to the dose of HA or calcium pyrophosphate dihydrate crystals, although larger doses of the latter crystal were required to produce equivalent effects. Not all particulates were effective mitogenic agents. Latex beads and diamond crystals had no effect. Monosodium urate crystals modestly stimulated and calcium urate crystals markedly stimulated nuclide uptake. The more complex crystals found in a naturally occurring condition (calcinosis) were as mitogenic as the pure synthetic HA. The synovial cell hyperplasia sometimes associated with crystals might be explained in part by their mitogenic activity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcinosis - metabolism</subject><subject>Calcium Pyrophosphate - pharmacology</subject><subject>Cells, Cultured</subject><subject>Crystallization</subject><subject>Diphosphates - pharmacology</subject><subject>Dogs</subject><subject>Dose-Response Relationship, Drug</subject><subject>Durapatite</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Hydroxyapatites - pharmacology</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Metals (hemochromatosis...)</subject><subject>Mitogens</subject><subject>Other metabolic disorders</subject><subject>Thymidine - metabolism</subject><subject>Uric Acid - pharmacology</subject><issn>0004-3591</issn><issn>1529-0131</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LwzAYxoMoc06v3oQexFtn0mRpcxzDL5gIMs_lTZq4SL9MWrT_vSkb05unvMnze948PAhdEjwnGCe34Lo5STOcpJgTfISmZJGIGBNKjtEUY8xiuhDkFJ15_xGuCV3QCZpwmogwTlH5bLvmXddWRdoYrTofNSbaDoVrvgdoobOdjqAuIgWlsn0VtYNr2m3j2y0EpbAjOk7KDb6DMtjrSPVl1ztdRBVUFZQWwpMuS3-OTkxA9MX-nKG3-7vN6jFevzw8rZbrWDHOcJxSzJSWSSaZSiTNQBZSGmmSghicFkoCJlxlQoHmQlDJUo4VMBgtOuOUztDNbm_rms9e-y6vrB8TQK2b3ucZIYQJwQI434HKNd47bfLW2QrckBOcj_Xmod78t95guNpv7mWliwO-7zPo13sdfGjMOKiV9Qcsy1LOxRhQ7LAvW-rhn0_z5evmT4QftXqWvQ</recordid><startdate>198406</startdate><enddate>198406</enddate><creator>Cheung, Herman S.</creator><creator>Story, Michael T.</creator><creator>Mccarty, Daniel J.</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198406</creationdate><title>Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells</title><author>Cheung, Herman S. ; Story, Michael T. ; Mccarty, Daniel J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4640-7304ceb28b4c2b38abdbbfbf2d1f07dcba016c89cae6993b4760ca4aeb28e8633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcinosis - metabolism</topic><topic>Calcium Pyrophosphate - pharmacology</topic><topic>Cells, Cultured</topic><topic>Crystallization</topic><topic>Diphosphates - pharmacology</topic><topic>Dogs</topic><topic>Dose-Response Relationship, Drug</topic><topic>Durapatite</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Hydroxyapatites - pharmacology</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Metals (hemochromatosis...)</topic><topic>Mitogens</topic><topic>Other metabolic disorders</topic><topic>Thymidine - metabolism</topic><topic>Uric Acid - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Cheung, Herman S.</creatorcontrib><creatorcontrib>Story, Michael T.</creatorcontrib><creatorcontrib>Mccarty, Daniel J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Arthritis and rheumatism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheung, Herman S.</au><au>Story, Michael T.</au><au>Mccarty, Daniel J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells</atitle><jtitle>Arthritis and rheumatism</jtitle><addtitle>Arthritis Rheum</addtitle><date>1984-06</date><risdate>1984</risdate><volume>27</volume><issue>6</issue><spage>668</spage><epage>674</epage><pages>668-674</pages><issn>0004-3591</issn><eissn>1529-0131</eissn><coden>ARHEAW</coden><abstract>Synthetic hydroxyapatite (HA) crystals in 1% serum stimulated 3H thymidine uptake into quiescent canine synovial fibroblasts and human foreskin fibroblast cultures, as did 10% serum. The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2—3 hours as compared with the effects produced by 10% serum. Stimulation of 3H thymidine uptake was proportional to the serum concentration used. HA crystals (50 μg/ml) stimulated nuclide uptake at each serum concentration used. 3H thymidine uptake was also proportional to the dose of HA or calcium pyrophosphate dihydrate crystals, although larger doses of the latter crystal were required to produce equivalent effects. Not all particulates were effective mitogenic agents. Latex beads and diamond crystals had no effect. Monosodium urate crystals modestly stimulated and calcium urate crystals markedly stimulated nuclide uptake. The more complex crystals found in a naturally occurring condition (calcinosis) were as mitogenic as the pure synthetic HA. The synovial cell hyperplasia sometimes associated with crystals might be explained in part by their mitogenic activity.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>6329235</pmid><doi>10.1002/art.1780270610</doi><tpages>7</tpages></addata></record> |
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| ispartof | Arthritis and rheumatism, 1984-06, Vol.27 (6), p.668-674 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Calcinosis - metabolism Calcium Pyrophosphate - pharmacology Cells, Cultured Crystallization Diphosphates - pharmacology Dogs Dose-Response Relationship, Drug Durapatite Fibroblasts - drug effects Fibroblasts - metabolism Humans Hydroxyapatites - pharmacology Medical sciences Metabolic diseases Metals (hemochromatosis...) Mitogens Other metabolic disorders Thymidine - metabolism Uric Acid - pharmacology |
| title | Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells |
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