Increased accuracy and speed of absorption cytometric DNA measurements by automatic corrections for nuclear darkness
We have developed a method of calculating the average local absorbance (ALA) of a nucleus from the integrated nuclear absorbance and area. One can use the ALA, along with nuclear areas measured at different point absorbance thresholds, to determine whether a nucleus is stained too lightly or too dar...
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| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1984-05, Vol.5 (3), p.217-227 |
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| container_title | Cytometry (New York, N.Y.) |
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| creator | Allison, David C. Lawrence, George N. Ridolpho, Paul F. O'Grady, Brian J. Rasch, Robert W. Rasch, Ellen M. |
| description | We have developed a method of calculating the average local absorbance (ALA) of a nucleus from the integrated nuclear absorbance and area. One can use the ALA, along with nuclear areas measured at different point absorbance thresholds, to determine whether a nucleus is stained too lightly or too darkly for accurate absorption measurements; this allows selection of an optimal light wavelength for the performance of these measurements. The ALA can also be used for automatic and instantaneous correction of integrated absorbance values from darkly stained cells. This allows the rapid measurement of the integrated absorbances of a large number of nuclei that are heterogeneous in stain intensity. Coefficients of variation of approximately 3% are obtained for the integrated absorbances of nuclei of nontransformed G0/G1 cells. This correction method can be applied with any image densitometer that generates both integrated absorbance and area values. |
| doi_str_mv | 10.1002/cyto.990050302 |
| format | Article |
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One can use the ALA, along with nuclear areas measured at different point absorbance thresholds, to determine whether a nucleus is stained too lightly or too darkly for accurate absorption measurements; this allows selection of an optimal light wavelength for the performance of these measurements. The ALA can also be used for automatic and instantaneous correction of integrated absorbance values from darkly stained cells. This allows the rapid measurement of the integrated absorbances of a large number of nuclei that are heterogeneous in stain intensity. Coefficients of variation of approximately 3% are obtained for the integrated absorbances of nuclei of nontransformed G0/G1 cells. This correction method can be applied with any image densitometer that generates both integrated absorbance and area values.</description><identifier>ISSN: 0196-4763</identifier><identifier>EISSN: 1097-0320</identifier><identifier>DOI: 10.1002/cyto.990050302</identifier><identifier>PMID: 6375996</identifier><identifier>CODEN: CYTODQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Absorption cytometry ; Animals ; Biological and medical sciences ; Bone Marrow Cells ; Cell nucleus ; Cell structures and functions ; Cytological Techniques ; DNA - analysis ; Feulgen stain ; Fundamental and applied biological sciences. Psychology ; Humans ; Interphase ; Leukocytes - analysis ; measurement of cellular DNA ; Mice ; Molecular and cellular biology ; Photometry - methods</subject><ispartof>Cytometry (New York, N.Y.), 1984-05, Vol.5 (3), p.217-227</ispartof><rights>Copyright © 1984 Wiley‐Liss, Inc.</rights><rights>1985 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4092-5218d06178767dabfe6a741d9419c9fe0ff2c2b66de08b9f5af042b7e6a274f33</citedby><cites>FETCH-LOGICAL-c4092-5218d06178767dabfe6a741d9419c9fe0ff2c2b66de08b9f5af042b7e6a274f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8934661$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6375996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allison, David C.</creatorcontrib><creatorcontrib>Lawrence, George N.</creatorcontrib><creatorcontrib>Ridolpho, Paul F.</creatorcontrib><creatorcontrib>O'Grady, Brian J.</creatorcontrib><creatorcontrib>Rasch, Robert W.</creatorcontrib><creatorcontrib>Rasch, Ellen M.</creatorcontrib><title>Increased accuracy and speed of absorption cytometric DNA measurements by automatic corrections for nuclear darkness</title><title>Cytometry (New York, N.Y.)</title><addtitle>Cytometry</addtitle><description>We have developed a method of calculating the average local absorbance (ALA) of a nucleus from the integrated nuclear absorbance and area. 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This correction method can be applied with any image densitometer that generates both integrated absorbance and area values.</description><subject>Absorption cytometry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells</subject><subject>Cell nucleus</subject><subject>Cell structures and functions</subject><subject>Cytological Techniques</subject><subject>DNA - analysis</subject><subject>Feulgen stain</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Interphase</subject><subject>Leukocytes - analysis</subject><subject>measurement of cellular DNA</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Photometry - methods</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1v2zAQxYmgRep8rNkKcCi6yTmSEimOgdM2AYJkSYdOwok6Amol0SUlBP7vS8OGO2Y64N7v3cdj7EbAWgDIW7ebw9pagAoUyDO2EmBNAUrCB7YCYXVRGq0-sYuUfgOA1aU6Z-damcpavWLz4-QiYaKOo3NLRLfjOHU8bSm3gufYphC3cx8mvl810hx7x--f7_iYbUukkaY58TbblizjnFUXYiS39yTuQ-TT4gbCyDuMfyZK6Yp99Dgkuj7WS_bz-7fXzUPx9PLjcXP3VLgSrCwqKeoOtDC10abD1pNGU4rOlsI66wm8l062WncEdWt9hR5K2ZqMSVN6pS7Z18PcbQx_F0pzM_bJ0TDgRGFJTS2EUFJWGVwfQBdDSpF8s439iHHXCGj2MTf735tTzNnw-Th5aUfqTvgx16x_OeqYHA4-4uT6dMJqq0qtRcbsAXvrB9q9s7TZ_Hp9-X_CPw0KmR4</recordid><startdate>198405</startdate><enddate>198405</enddate><creator>Allison, David C.</creator><creator>Lawrence, George N.</creator><creator>Ridolpho, Paul F.</creator><creator>O'Grady, Brian J.</creator><creator>Rasch, Robert W.</creator><creator>Rasch, Ellen M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198405</creationdate><title>Increased accuracy and speed of absorption cytometric DNA measurements by automatic corrections for nuclear darkness</title><author>Allison, David C. ; Lawrence, George N. ; Ridolpho, Paul F. ; O'Grady, Brian J. ; Rasch, Robert W. ; Rasch, Ellen M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4092-5218d06178767dabfe6a741d9419c9fe0ff2c2b66de08b9f5af042b7e6a274f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Absorption cytometry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells</topic><topic>Cell nucleus</topic><topic>Cell structures and functions</topic><topic>Cytological Techniques</topic><topic>DNA - analysis</topic><topic>Feulgen stain</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Interphase</topic><topic>Leukocytes - analysis</topic><topic>measurement of cellular DNA</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Photometry - methods</topic><toplevel>online_resources</toplevel><creatorcontrib>Allison, David C.</creatorcontrib><creatorcontrib>Lawrence, George N.</creatorcontrib><creatorcontrib>Ridolpho, Paul F.</creatorcontrib><creatorcontrib>O'Grady, Brian J.</creatorcontrib><creatorcontrib>Rasch, Robert W.</creatorcontrib><creatorcontrib>Rasch, Ellen M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allison, David C.</au><au>Lawrence, George N.</au><au>Ridolpho, Paul F.</au><au>O'Grady, Brian J.</au><au>Rasch, Robert W.</au><au>Rasch, Ellen M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased accuracy and speed of absorption cytometric DNA measurements by automatic corrections for nuclear darkness</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1984-05</date><risdate>1984</risdate><volume>5</volume><issue>3</issue><spage>217</spage><epage>227</epage><pages>217-227</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>We have developed a method of calculating the average local absorbance (ALA) of a nucleus from the integrated nuclear absorbance and area. 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| fulltext | fulltext |
| identifier | ISSN: 0196-4763 |
| ispartof | Cytometry (New York, N.Y.), 1984-05, Vol.5 (3), p.217-227 |
| issn | 0196-4763 1097-0320 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81113225 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Absorption cytometry Animals Biological and medical sciences Bone Marrow Cells Cell nucleus Cell structures and functions Cytological Techniques DNA - analysis Feulgen stain Fundamental and applied biological sciences. Psychology Humans Interphase Leukocytes - analysis measurement of cellular DNA Mice Molecular and cellular biology Photometry - methods |
| title | Increased accuracy and speed of absorption cytometric DNA measurements by automatic corrections for nuclear darkness |
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