Perfusion of the rat ovary in vitro: methodology, induction of ovulation, and pattern of steroidogenesis
A method for perfusion of the rat ovary in vitro for the study of ovulation was developed and characterized. Immature rats (27-29 days old) were primed with 20 IU of pregnant mare's serum gonadotropin (PMSG). Two days later a laparotomy was performed, the aorta and vena cava were cannulated, th...
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| Veröffentlicht in: | Biology of reproduction 1984-06, Vol.30 (5), p.1135-1141 |
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| creator | KOOS, R. D JACCARINO, F. J MAGARIL, R. A LE MAIRE, W. J |
| description | A method for perfusion of the rat ovary in vitro for the study of ovulation was developed and characterized. Immature rats
(27-29 days old) were primed with 20 IU of pregnant mare's serum gonadotropin (PMSG). Two days later a laparotomy was performed,
the aorta and vena cava were cannulated, the right ovary was isolated, and all vessels connecting with the aorta and vena
cava except for the right ovarian artery and vein were ligated. The preparation was placed in the perfusion apparatus and
perfused for up to 20 h with oxygenated Medium 199 containing 4% bovine serum albumin (37 degrees C, pH 7.4). The perfusion
pressure was 70-90 mm Hg and the average flow was 1 ml . min . ovary. Ovulation was confirmed by oocyte recovery from the
apparatus. Nine ovaries were perfused without further treatment (controls); only 1 ovulation occurred in this group. Nine
ovaries received luteinizing hormone (LH; 0.1 microgram/ml) 1 h after the start of perfusion; 71 ovulations resulted (range,
3-13/ovary). Ovulations did not begin before 9 h after LH administration. Samples of medium were taken frequently for measurement
of progesterone, estradiol and androstenedione. Levels of all three steroids rose rapidly and markedly in response to LH but
increased only slightly in control perfusions. This study demonstrates that ovulation in the rat ovary can be induced in vitro
and provides a basis for further studies on the mechanism of ovulation. |
| doi_str_mv | 10.1095/biolreprod30.5.1135 |
| format | Article |
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(27-29 days old) were primed with 20 IU of pregnant mare's serum gonadotropin (PMSG). Two days later a laparotomy was performed,
the aorta and vena cava were cannulated, the right ovary was isolated, and all vessels connecting with the aorta and vena
cava except for the right ovarian artery and vein were ligated. The preparation was placed in the perfusion apparatus and
perfused for up to 20 h with oxygenated Medium 199 containing 4% bovine serum albumin (37 degrees C, pH 7.4). The perfusion
pressure was 70-90 mm Hg and the average flow was 1 ml . min . ovary. Ovulation was confirmed by oocyte recovery from the
apparatus. Nine ovaries were perfused without further treatment (controls); only 1 ovulation occurred in this group. Nine
ovaries received luteinizing hormone (LH; 0.1 microgram/ml) 1 h after the start of perfusion; 71 ovulations resulted (range,
3-13/ovary). Ovulations did not begin before 9 h after LH administration. Samples of medium were taken frequently for measurement
of progesterone, estradiol and androstenedione. Levels of all three steroids rose rapidly and markedly in response to LH but
increased only slightly in control perfusions. This study demonstrates that ovulation in the rat ovary can be induced in vitro
and provides a basis for further studies on the mechanism of ovulation.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod30.5.1135</identifier><identifier>PMID: 6733206</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Androstenedione - biosynthesis ; Animals ; Biological and medical sciences ; Chorionic Gonadotropin - pharmacology ; Estradiol - biosynthesis ; Female ; Fundamental and applied biological sciences. Psychology ; Gonadotropins, Equine - pharmacology ; In Vitro Techniques ; Luteinizing Hormone - pharmacology ; Mammalian female genital system ; Morphology. Physiology ; Ovary - drug effects ; Ovary - physiology ; Ovulation Induction ; Perfusion - methods ; Progesterone - biosynthesis ; Rats ; Rats, Inbred Strains ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1984-06, Vol.30 (5), p.1135-1141</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9085508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6733206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOOS, R. D</creatorcontrib><creatorcontrib>JACCARINO, F. J</creatorcontrib><creatorcontrib>MAGARIL, R. A</creatorcontrib><creatorcontrib>LE MAIRE, W. J</creatorcontrib><title>Perfusion of the rat ovary in vitro: methodology, induction of ovulation, and pattern of steroidogenesis</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A method for perfusion of the rat ovary in vitro for the study of ovulation was developed and characterized. Immature rats
(27-29 days old) were primed with 20 IU of pregnant mare's serum gonadotropin (PMSG). Two days later a laparotomy was performed,
the aorta and vena cava were cannulated, the right ovary was isolated, and all vessels connecting with the aorta and vena
cava except for the right ovarian artery and vein were ligated. The preparation was placed in the perfusion apparatus and
perfused for up to 20 h with oxygenated Medium 199 containing 4% bovine serum albumin (37 degrees C, pH 7.4). The perfusion
pressure was 70-90 mm Hg and the average flow was 1 ml . min . ovary. Ovulation was confirmed by oocyte recovery from the
apparatus. Nine ovaries were perfused without further treatment (controls); only 1 ovulation occurred in this group. Nine
ovaries received luteinizing hormone (LH; 0.1 microgram/ml) 1 h after the start of perfusion; 71 ovulations resulted (range,
3-13/ovary). Ovulations did not begin before 9 h after LH administration. Samples of medium were taken frequently for measurement
of progesterone, estradiol and androstenedione. Levels of all three steroids rose rapidly and markedly in response to LH but
increased only slightly in control perfusions. This study demonstrates that ovulation in the rat ovary can be induced in vitro
and provides a basis for further studies on the mechanism of ovulation.</description><subject>Androstenedione - biosynthesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Estradiol - biosynthesis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonadotropins, Equine - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Morphology. Physiology</subject><subject>Ovary - drug effects</subject><subject>Ovary - physiology</subject><subject>Ovulation Induction</subject><subject>Perfusion - methods</subject><subject>Progesterone - biosynthesis</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUUtP3DAQthAV3QK_oELyAfW02Y7t2JtwQ6uWVlqpPZRz5CTjjSsn3trORvz7BohgLvP4vnkT8pnBhkEpv9bWu4DH4FsBG7lhTMgzsmKSl9mWq-KcrABAZUIo8ZF8ivEvAMsFFxfkQm2F4KBWpPuNwYzR-oF6Q1OHNOhE_UmHJ2oHerIp-DvaY-p8650_PK3ncDs2acnwp9HpZ2dN9dDSo04JwwsSZ8Pb1h9wwGjjFflgtIt4vehL8vj925_dj2z_6-Hn7n6fdXyrUlZAUUpTM8lyJuu2VrxWBYKBedqtgiLHnDeAjVDAOeNY5rrWdWnAMNmUphGX5Mtr3fku_0aMqeptbNA5PaAfY1WwFxEz8WYhjnWPbXUMtp-3rpbTzPjtguvYaGeCHhob32glFFJC8d6vs4dusgGr2Gvn5qKimqZJQCWr58-I_78TgvM</recordid><startdate>198406</startdate><enddate>198406</enddate><creator>KOOS, R. D</creator><creator>JACCARINO, F. J</creator><creator>MAGARIL, R. A</creator><creator>LE MAIRE, W. J</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198406</creationdate><title>Perfusion of the rat ovary in vitro: methodology, induction of ovulation, and pattern of steroidogenesis</title><author>KOOS, R. D ; JACCARINO, F. J ; MAGARIL, R. A ; LE MAIRE, W. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h276t-80895fb151415bdb62b68e0f020676084e42c0ec3602212e94abab9f0f15c9fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Androstenedione - biosynthesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Estradiol - biosynthesis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonadotropins, Equine - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Morphology. Physiology</topic><topic>Ovary - drug effects</topic><topic>Ovary - physiology</topic><topic>Ovulation Induction</topic><topic>Perfusion - methods</topic><topic>Progesterone - biosynthesis</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOOS, R. D</creatorcontrib><creatorcontrib>JACCARINO, F. J</creatorcontrib><creatorcontrib>MAGARIL, R. A</creatorcontrib><creatorcontrib>LE MAIRE, W. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOOS, R. D</au><au>JACCARINO, F. J</au><au>MAGARIL, R. A</au><au>LE MAIRE, W. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perfusion of the rat ovary in vitro: methodology, induction of ovulation, and pattern of steroidogenesis</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1984-06</date><risdate>1984</risdate><volume>30</volume><issue>5</issue><spage>1135</spage><epage>1141</epage><pages>1135-1141</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A method for perfusion of the rat ovary in vitro for the study of ovulation was developed and characterized. Immature rats
(27-29 days old) were primed with 20 IU of pregnant mare's serum gonadotropin (PMSG). Two days later a laparotomy was performed,
the aorta and vena cava were cannulated, the right ovary was isolated, and all vessels connecting with the aorta and vena
cava except for the right ovarian artery and vein were ligated. The preparation was placed in the perfusion apparatus and
perfused for up to 20 h with oxygenated Medium 199 containing 4% bovine serum albumin (37 degrees C, pH 7.4). The perfusion
pressure was 70-90 mm Hg and the average flow was 1 ml . min . ovary. Ovulation was confirmed by oocyte recovery from the
apparatus. Nine ovaries were perfused without further treatment (controls); only 1 ovulation occurred in this group. Nine
ovaries received luteinizing hormone (LH; 0.1 microgram/ml) 1 h after the start of perfusion; 71 ovulations resulted (range,
3-13/ovary). Ovulations did not begin before 9 h after LH administration. Samples of medium were taken frequently for measurement
of progesterone, estradiol and androstenedione. Levels of all three steroids rose rapidly and markedly in response to LH but
increased only slightly in control perfusions. This study demonstrates that ovulation in the rat ovary can be induced in vitro
and provides a basis for further studies on the mechanism of ovulation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>6733206</pmid><doi>10.1095/biolreprod30.5.1135</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1984-06, Vol.30 (5), p.1135-1141 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81111113 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Androstenedione - biosynthesis Animals Biological and medical sciences Chorionic Gonadotropin - pharmacology Estradiol - biosynthesis Female Fundamental and applied biological sciences. Psychology Gonadotropins, Equine - pharmacology In Vitro Techniques Luteinizing Hormone - pharmacology Mammalian female genital system Morphology. Physiology Ovary - drug effects Ovary - physiology Ovulation Induction Perfusion - methods Progesterone - biosynthesis Rats Rats, Inbred Strains Vertebrates: reproduction |
| title | Perfusion of the rat ovary in vitro: methodology, induction of ovulation, and pattern of steroidogenesis |
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