A different approach to immersion fixation of human dental pulp and odontoblast processes
A low-speed diamond-edge rotary saw microtome was used to prepare freshly extracted human teeth for fixation. The teeth were sliced transversely 900 μm thick using saline as a coolant; the slices were immersed in glutaraldehyde fixative within 10 min of tooth extraction. After flat-embedding in Aral...
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| Veröffentlicht in: | Archives of oral biology 1984, Vol.29 (3), p.243-248 |
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| container_title | Archives of oral biology |
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| creator | Gaynor, M.K. Weber, D.F. Everingham, J. |
| description | A low-speed diamond-edge rotary saw microtome was used to prepare freshly extracted human teeth for fixation. The teeth were sliced transversely 900 μm thick using saline as a coolant; the slices were immersed in glutaraldehyde fixative within 10 min of tooth extraction. After flat-embedding in Araldite, sections were reduced to 70 μm thickness and examined with light microscopy. Selected areas were processed for electron microscopy. In the light microscope, the entire pulp appeared to be well fixed. Electron microscopy showed that the contents of most of the tubules near the predentine were well fixed and microfilaments and microtubules were present in odontoblast processes. The quality of fixation with this method was at least as good as other methods applicable to teeth with the advantage that the integrity of the entire dental pulp was preserved. |
| doi_str_mv | 10.1016/0003-9969(84)90062-1 |
| format | Article |
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The teeth were sliced transversely 900 μm thick using saline as a coolant; the slices were immersed in glutaraldehyde fixative within 10 min of tooth extraction. After flat-embedding in Araldite, sections were reduced to 70 μm thickness and examined with light microscopy. Selected areas were processed for electron microscopy. In the light microscope, the entire pulp appeared to be well fixed. Electron microscopy showed that the contents of most of the tubules near the predentine were well fixed and microfilaments and microtubules were present in odontoblast processes. The quality of fixation with this method was at least as good as other methods applicable to teeth with the advantage that the integrity of the entire dental pulp was preserved.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/0003-9969(84)90062-1</identifier><identifier>PMID: 6375644</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Dental Pulp - ultrastructure ; Dentin - ultrastructure ; Dentistry ; Histological Techniques ; Humans ; Microscopy, Electron ; Middle Aged ; Molar ; Odontoblasts - ultrastructure</subject><ispartof>Archives of oral biology, 1984, Vol.29 (3), p.243-248</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-a376f9c2d0e93cbc6462c542e5a00318dd2d2619e1353f59445205e9fd4f5b6c3</citedby><cites>FETCH-LOGICAL-c357t-a376f9c2d0e93cbc6462c542e5a00318dd2d2619e1353f59445205e9fd4f5b6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9969(84)90062-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6375644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaynor, M.K.</creatorcontrib><creatorcontrib>Weber, D.F.</creatorcontrib><creatorcontrib>Everingham, J.</creatorcontrib><title>A different approach to immersion fixation of human dental pulp and odontoblast processes</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>A low-speed diamond-edge rotary saw microtome was used to prepare freshly extracted human teeth for fixation. The teeth were sliced transversely 900 μm thick using saline as a coolant; the slices were immersed in glutaraldehyde fixative within 10 min of tooth extraction. After flat-embedding in Araldite, sections were reduced to 70 μm thickness and examined with light microscopy. Selected areas were processed for electron microscopy. In the light microscope, the entire pulp appeared to be well fixed. Electron microscopy showed that the contents of most of the tubules near the predentine were well fixed and microfilaments and microtubules were present in odontoblast processes. The quality of fixation with this method was at least as good as other methods applicable to teeth with the advantage that the integrity of the entire dental pulp was preserved.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Dental Pulp - ultrastructure</subject><subject>Dentin - ultrastructure</subject><subject>Dentistry</subject><subject>Histological Techniques</subject><subject>Humans</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Molar</subject><subject>Odontoblasts - ultrastructure</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVocRy3_yABnUp72EZafezqEgimTQOGXpJDTkKWRlhhd7WRdkP67yPHxseeZoZ5552ZB6FLSn5SQuU1IYRVSkn1veU_FCGyrugZWtK2URUVRH5Cy5PkHF3k_FxKISVdoIVkjZCcL9HTLXbBe0gwTNiMY4rG7vAUceh7SDnEAfvwZqZ9Ej3ezb0ZsCti0-Fx7kZsBoeji8MUt53JEy4OFnKG_AV99qbL8PUYV-jx96-H9Z9q8_fufn27qSwTzVQZ1kivbO0IKGa3VnJZW8FrEKYcT1vnaldLqoAywbxQnIuaCFDecS-20rIV-nbwLZtfZsiT7kO20HVmgDhn3VJKGqZEEfKD0KaYcwKvxxR6k_5pSvSeqN7j0ntcuuX6g6imZezq6D9ve3CnoSPC0r859KE8-Rog6WwDDBZcSGAn7WL4_4J36zKFjg</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Gaynor, M.K.</creator><creator>Weber, D.F.</creator><creator>Everingham, J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1984</creationdate><title>A different approach to immersion fixation of human dental pulp and odontoblast processes</title><author>Gaynor, M.K. ; Weber, D.F. ; Everingham, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-a376f9c2d0e93cbc6462c542e5a00318dd2d2619e1353f59445205e9fd4f5b6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Dental Pulp - ultrastructure</topic><topic>Dentin - ultrastructure</topic><topic>Dentistry</topic><topic>Histological Techniques</topic><topic>Humans</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Molar</topic><topic>Odontoblasts - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaynor, M.K.</creatorcontrib><creatorcontrib>Weber, D.F.</creatorcontrib><creatorcontrib>Everingham, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaynor, M.K.</au><au>Weber, D.F.</au><au>Everingham, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A different approach to immersion fixation of human dental pulp and odontoblast processes</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>1984</date><risdate>1984</risdate><volume>29</volume><issue>3</issue><spage>243</spage><epage>248</epage><pages>243-248</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>A low-speed diamond-edge rotary saw microtome was used to prepare freshly extracted human teeth for fixation. The teeth were sliced transversely 900 μm thick using saline as a coolant; the slices were immersed in glutaraldehyde fixative within 10 min of tooth extraction. After flat-embedding in Araldite, sections were reduced to 70 μm thickness and examined with light microscopy. Selected areas were processed for electron microscopy. In the light microscope, the entire pulp appeared to be well fixed. Electron microscopy showed that the contents of most of the tubules near the predentine were well fixed and microfilaments and microtubules were present in odontoblast processes. The quality of fixation with this method was at least as good as other methods applicable to teeth with the advantage that the integrity of the entire dental pulp was preserved.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6375644</pmid><doi>10.1016/0003-9969(84)90062-1</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9969 |
| ispartof | Archives of oral biology, 1984, Vol.29 (3), p.243-248 |
| issn | 0003-9969 1879-1506 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adolescent Adult Dental Pulp - ultrastructure Dentin - ultrastructure Dentistry Histological Techniques Humans Microscopy, Electron Middle Aged Molar Odontoblasts - ultrastructure |
| title | A different approach to immersion fixation of human dental pulp and odontoblast processes |
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