Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus
A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpenelactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alk...
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| Veröffentlicht in: | Analytical biochemistry 1984-01, Vol.138 (1), p.19-24 |
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| container_title | Analytical biochemistry |
| container_volume | 138 |
| creator | Jourdan, Pablo S. Mansell, Richard L. Oliver, Diane G. Weiler, Elmar W. |
| description | A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpenelactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forcedair microplate incubator. |
| doi_str_mv | 10.1016/0003-2697(84)90762-0 |
| format | Article |
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As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forcedair microplate incubator.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(84)90762-0</identifier><identifier>PMID: 6375457</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alkaline Phosphatase ; Analysis of complex biological substances ; Analytical, structural and metabolic biochemistry ; Beverages - analysis ; Biological and medical sciences ; Citrus ; Citrus - analysis ; citrus bitter principle ; Cross Reactions ; enzyme immunoassay ; Fundamental and applied biological sciences. 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As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forcedair microplate incubator.</description><subject>Alkaline Phosphatase</subject><subject>Analysis of complex biological substances</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Beverages - analysis</subject><subject>Biological and medical sciences</subject><subject>Citrus</subject><subject>Citrus - analysis</subject><subject>citrus bitter principle</subject><subject>Cross Reactions</subject><subject>enzyme immunoassay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoenzyme Techniques</subject><subject>limonin</subject><subject>Limonins</subject><subject>Plants and fungi</subject><subject>Triterpenes - isolation & purification</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo6-zqP1DIQWQ9tFY-Op1cFmTwCxa86FFCOl1ho93JbNK9MP56e5xhjgoFdajnfSkeQl4weMuAqXcAIBquTHet5RsDneINPCIbBkY1IMA8Jpsz8pRc1voTgDHZqgtyoUTXyrbbkB_bPO1wjnN8QFrzGAe6u3MVKabf-wmbMaZfONA4TUvKrla3pyEXOt8hvV9cmmOI3s0xJ5oDHeOUU0x0HR_nstRn5ElwY8Xnp31Fvn_88G37ubn9-unL9v1t4yXr5kZwFLrvNeci6L7lweleoOm544Nr21aEAD0MSkpQg-BGtV1g2iAT0EoPTlyR18feXcn3C9bZTrF6HEeXMC_VasZAcW3-CzJhWuBar6A8gr7kWgsGuytxcmVvGdiDfntwaw9urZb2r34La-zlqX_pJxzOoZPv9f7qdHfVuzEUl3ysZ0wbLZk-1NwcMVylPUQstvqIyeMQC_rZDjn--48_OfahPA</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Jourdan, Pablo S.</creator><creator>Mansell, Richard L.</creator><creator>Oliver, Diane G.</creator><creator>Weiler, Elmar W.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus</title><author>Jourdan, Pablo S. ; Mansell, Richard L. ; Oliver, Diane G. ; Weiler, Elmar W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-32e38bb8223f8b52fa8b3e9b2a2da5553ff0b0d64406d329657f189e13054c0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Alkaline Phosphatase</topic><topic>Analysis of complex biological substances</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Beverages - analysis</topic><topic>Biological and medical sciences</topic><topic>Citrus</topic><topic>Citrus - analysis</topic><topic>citrus bitter principle</topic><topic>Cross Reactions</topic><topic>enzyme immunoassay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoenzyme Techniques</topic><topic>limonin</topic><topic>Limonins</topic><topic>Plants and fungi</topic><topic>Triterpenes - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jourdan, Pablo S.</creatorcontrib><creatorcontrib>Mansell, Richard L.</creatorcontrib><creatorcontrib>Oliver, Diane G.</creatorcontrib><creatorcontrib>Weiler, Elmar W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jourdan, Pablo S.</au><au>Mansell, Richard L.</au><au>Oliver, Diane G.</au><au>Weiler, Elmar W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>138</volume><issue>1</issue><spage>19</spage><epage>24</epage><pages>19-24</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A solid-phase enzyme immunoassay for the quantitative determination of the bitter triterpenelactone, limonin, in citrus juice samples is described. As little as 0.1 ppm of limonin can be detected. Quantitative results are available within 1 h of total assay time. The assay makes use of a limonin-alkaline phosphatase tracer of high immunoreactivity and has been semiautomated using antibody-coated polystyrene microcuvettes, a vertical light path photometer, and a forcedair microplate incubator.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6375457</pmid><doi>10.1016/0003-2697(84)90762-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1984-01, Vol.138 (1), p.19-24 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase Analysis of complex biological substances Analytical, structural and metabolic biochemistry Beverages - analysis Biological and medical sciences Citrus Citrus - analysis citrus bitter principle Cross Reactions enzyme immunoassay Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques limonin Limonins Plants and fungi Triterpenes - isolation & purification |
| title | Competitive solid phase enzyme-linked immunoassay for the quantification of limonin in citrus |
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