Inhibitory effect of ionized free intracellular calcium enhanced by ruthenium red and m-chloro-carbonylcyanide phenyl hydrazon on the evoked release of acetylcholine
In order to understand the relationship between the free ionized calcium concentration in the axon terminals and the transmitter release we have investigated the effect of ruthenium red (RuR) and m-chloro-carbonylcyanide phenyl hydrazon (CCCP), mitochondrial uncoupler agents on the liberation of ace...
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| Veröffentlicht in: | Biochemical pharmacology 1987-11, Vol.36 (21), p.3683-3687 |
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| container_title | Biochemical pharmacology |
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| creator | Bernath, Sandor Vizi, E.Sylvester |
| description | In order to understand the relationship between the free ionized calcium concentration in the axon terminals and the transmitter release we have investigated the effect of ruthenium red (RuR) and
m-chloro-carbonylcyanide phenyl hydrazon (CCCP), mitochondrial uncoupler agents on the liberation of acetylcholine from myenteric plexus of guinea-pig ileum. Both compounds are able to enhance intracellular free Ca
2+.
In the presence of RuR and CCCP the spontaneous release of radioactivity from isolated myentericplexus preparation, previously loaded with (methyl-3
H)-choline chloride was significantly enhanced while that evoked by electrical stimulation was decreased. Atropine did not affect the electrically evoked release in the presence of CCCP, indicating that in the effect of CCCP the presynaptic muscarinic receptors mediated negative feedback modulation does not play any role. Our findings are consistent with the hypothesis that the enhanced level of intracellular free Ca
2+ enhances the spontaneous while depresses the electrically evoked release of transmitter. |
| doi_str_mv | 10.1016/0006-2952(87)90020-7 |
| format | Article |
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m-chloro-carbonylcyanide phenyl hydrazon (CCCP), mitochondrial uncoupler agents on the liberation of acetylcholine from myenteric plexus of guinea-pig ileum. Both compounds are able to enhance intracellular free Ca
2+.
In the presence of RuR and CCCP the spontaneous release of radioactivity from isolated myentericplexus preparation, previously loaded with (methyl-3
H)-choline chloride was significantly enhanced while that evoked by electrical stimulation was decreased. Atropine did not affect the electrically evoked release in the presence of CCCP, indicating that in the effect of CCCP the presynaptic muscarinic receptors mediated negative feedback modulation does not play any role. Our findings are consistent with the hypothesis that the enhanced level of intracellular free Ca
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m-chloro-carbonylcyanide phenyl hydrazon (CCCP), mitochondrial uncoupler agents on the liberation of acetylcholine from myenteric plexus of guinea-pig ileum. Both compounds are able to enhance intracellular free Ca
2+.
In the presence of RuR and CCCP the spontaneous release of radioactivity from isolated myentericplexus preparation, previously loaded with (methyl-3
H)-choline chloride was significantly enhanced while that evoked by electrical stimulation was decreased. Atropine did not affect the electrically evoked release in the presence of CCCP, indicating that in the effect of CCCP the presynaptic muscarinic receptors mediated negative feedback modulation does not play any role. Our findings are consistent with the hypothesis that the enhanced level of intracellular free Ca
2+ enhances the spontaneous while depresses the electrically evoked release of transmitter.</description><subject>Acetylcholine - metabolism</subject><subject>Animals</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology</subject><subject>Electric Stimulation</subject><subject>Guinea Pigs</subject><subject>In Vitro Techniques</subject><subject>Myenteric Plexus - analysis</subject><subject>Myenteric Plexus - metabolism</subject><subject>Nitriles - pharmacology</subject><subject>Potassium - metabolism</subject><subject>Ruthenium - pharmacology</subject><subject>Ruthenium Red - pharmacology</subject><subject>Stimulation, Chemical</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFTEQhYMo43X0DRSyEl20VpL-SW-EYfBnYMCNrkM6XaGj6eSadA_0vI_vadp7maVCIKTOOVVJPkJeMnjHgLXvAaCteN_wN7J72wNwqLpH5MBkJ0q5lY_J4cHylDzL-cd-lC27IBe8rhvRwIH8vgmTG9wS00bRWjQLjZa6GNw9jtQmROrCkrRB71evEzXaG7fOFMOkgymeYaNpXSYMezWVgg4jnSsz-ZhiZXQaYti82XRwI9JjMW6eTtuY9H0MtKySpXgXf5ZoQo86436FMnEpsSl6F_A5eWK1z_jivF-S758-frv-Ut1-_XxzfXVbGQFiqThYaLiVPePWDMJgzQX0VjQjt0wOIDpRZNBjL03HamBiqOu-7wXr9SAaKy7J61PfY4q_VsyLml3en64DxjUryRhwydr_Glkt61ZAV4z1yWhSzDmhVcfkZp02xUDtGNUORe2MlOzUX4xqj70691-HGceH0Jlb0T-cdCy_cecwqWwc7jxcKgzVGN2_B_wBGDOu9w</recordid><startdate>19871101</startdate><enddate>19871101</enddate><creator>Bernath, Sandor</creator><creator>Vizi, E.Sylvester</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19871101</creationdate><title>Inhibitory effect of ionized free intracellular calcium enhanced by ruthenium red and m-chloro-carbonylcyanide phenyl hydrazon on the evoked release of acetylcholine</title><author>Bernath, Sandor ; Vizi, E.Sylvester</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-20f052f8912fcb3ce42309f35d2f18b037352f0ad98c714013b44999319ab35f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Acetylcholine - metabolism</topic><topic>Animals</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology</topic><topic>Electric Stimulation</topic><topic>Guinea Pigs</topic><topic>In Vitro Techniques</topic><topic>Myenteric Plexus - analysis</topic><topic>Myenteric Plexus - metabolism</topic><topic>Nitriles - pharmacology</topic><topic>Potassium - metabolism</topic><topic>Ruthenium - pharmacology</topic><topic>Ruthenium Red - pharmacology</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bernath, Sandor</creatorcontrib><creatorcontrib>Vizi, E.Sylvester</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bernath, Sandor</au><au>Vizi, E.Sylvester</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of ionized free intracellular calcium enhanced by ruthenium red and m-chloro-carbonylcyanide phenyl hydrazon on the evoked release of acetylcholine</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1987-11-01</date><risdate>1987</risdate><volume>36</volume><issue>21</issue><spage>3683</spage><epage>3687</epage><pages>3683-3687</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>In order to understand the relationship between the free ionized calcium concentration in the axon terminals and the transmitter release we have investigated the effect of ruthenium red (RuR) and
m-chloro-carbonylcyanide phenyl hydrazon (CCCP), mitochondrial uncoupler agents on the liberation of acetylcholine from myenteric plexus of guinea-pig ileum. Both compounds are able to enhance intracellular free Ca
2+.
In the presence of RuR and CCCP the spontaneous release of radioactivity from isolated myentericplexus preparation, previously loaded with (methyl-3
H)-choline chloride was significantly enhanced while that evoked by electrical stimulation was decreased. Atropine did not affect the electrically evoked release in the presence of CCCP, indicating that in the effect of CCCP the presynaptic muscarinic receptors mediated negative feedback modulation does not play any role. Our findings are consistent with the hypothesis that the enhanced level of intracellular free Ca
2+ enhances the spontaneous while depresses the electrically evoked release of transmitter.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>2445350</pmid><doi>10.1016/0006-2952(87)90020-7</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 1987-11, Vol.36 (21), p.3683-3687 |
| issn | 0006-2952 1873-2968 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acetylcholine - metabolism Animals Calcium - analysis Calcium - metabolism Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology Electric Stimulation Guinea Pigs In Vitro Techniques Myenteric Plexus - analysis Myenteric Plexus - metabolism Nitriles - pharmacology Potassium - metabolism Ruthenium - pharmacology Ruthenium Red - pharmacology Stimulation, Chemical |
| title | Inhibitory effect of ionized free intracellular calcium enhanced by ruthenium red and m-chloro-carbonylcyanide phenyl hydrazon on the evoked release of acetylcholine |
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