A sluicing device for reproducibly measuring the strength of attachment of cultured cells to their substrate
We assessed the strength of attachment of cultured human vascular endothelial cells to tissue culture plastic by controlled sluicing of cells, grown on multiwell plates, with isotonic saline using a specially designed nozzle attached to a reciprocating pump. Cells which were not dislodged were tryps...
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| Veröffentlicht in: | Analytical biochemistry 1987-07, Vol.164 (1), p.35-38 |
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| container_title | Analytical biochemistry |
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| creator | Dudman, Nicholas P.B. Hicks, Christine Wilcken, David E.L. |
| description | We assessed the strength of attachment of cultured human vascular endothelial cells to tissue culture plastic by controlled sluicing of cells, grown on multiwell plates, with isotonic saline using a specially designed nozzle attached to a reciprocating pump. Cells which were not dislodged were trypsinized and counted. The percentage of cells dislodged was proportional to the pump speed and to the volume of saline pumped. For given pump settings the proportion of cells detached was reproducible. The percentage of cells detached by sluicing increased at lower cell densities and decreased when the duration of trypsinizing of cells to be plated in multiwells, prior to sluicing, was increased. Our system is suitable for evaluating effects of biochemical interventions on the strength of cell attachment to the substrate. |
| doi_str_mv | 10.1016/0003-2697(87)90363-0 |
| format | Article |
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Cells which were not dislodged were trypsinized and counted. The percentage of cells dislodged was proportional to the pump speed and to the volume of saline pumped. For given pump settings the proportion of cells detached was reproducible. The percentage of cells detached by sluicing increased at lower cell densities and decreased when the duration of trypsinizing of cells to be plated in multiwells, prior to sluicing, was increased. Our system is suitable for evaluating effects of biochemical interventions on the strength of cell attachment to the substrate.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(87)90363-0</identifier><identifier>PMID: 3314579</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animal cells ; Biological and medical sciences ; Biotechnology ; blood vessels ; Cell Adhesion ; Cell Count ; Cell cultures. Hybridization. Fusion ; cell density ; cell detachment ; Cell hybridization, cell fusion, hybridomas ; Cells, Cultured ; controlled sluicing ; Cytological Techniques - instrumentation ; endothelial cells ; endothelium ; Endothelium - cytology ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrostatic Pressure ; man ; Methods. Procedures. Technologies ; Molecular and cellular biology ; Sodium Chloride ; Solutions ; Trypsin - pharmacology ; trypsin effects ; Vacuum</subject><ispartof>Analytical biochemistry, 1987-07, Vol.164 (1), p.35-38</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d334cea442623094e8cabee2f750c130df62bd7a4eedaeff1543b663863a12d53</citedby><cites>FETCH-LOGICAL-c417t-d334cea442623094e8cabee2f750c130df62bd7a4eedaeff1543b663863a12d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(87)90363-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7689219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3314579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dudman, Nicholas P.B.</creatorcontrib><creatorcontrib>Hicks, Christine</creatorcontrib><creatorcontrib>Wilcken, David E.L.</creatorcontrib><title>A sluicing device for reproducibly measuring the strength of attachment of cultured cells to their substrate</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>We assessed the strength of attachment of cultured human vascular endothelial cells to tissue culture plastic by controlled sluicing of cells, grown on multiwell plates, with isotonic saline using a specially designed nozzle attached to a reciprocating pump. Cells which were not dislodged were trypsinized and counted. The percentage of cells dislodged was proportional to the pump speed and to the volume of saline pumped. For given pump settings the proportion of cells detached was reproducible. The percentage of cells detached by sluicing increased at lower cell densities and decreased when the duration of trypsinizing of cells to be plated in multiwells, prior to sluicing, was increased. Our system is suitable for evaluating effects of biochemical interventions on the strength of cell attachment to the substrate.</description><subject>Animal cells</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>blood vessels</subject><subject>Cell Adhesion</subject><subject>Cell Count</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>cell density</subject><subject>cell detachment</subject><subject>Cell hybridization, cell fusion, hybridomas</subject><subject>Cells, Cultured</subject><subject>controlled sluicing</subject><subject>Cytological Techniques - instrumentation</subject><subject>endothelial cells</subject><subject>endothelium</subject><subject>Endothelium - cytology</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrostatic Pressure</subject><subject>man</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Sodium Chloride</subject><subject>Solutions</subject><subject>Trypsin - pharmacology</subject><subject>trypsin effects</subject><subject>Vacuum</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctqHTEMhk1pSU7TvEELXoTQLiaVxx57ZlMIIb1AIJtkbTy2nOMyl8SXQN6-MzmHs0xXktD3C-kXIZ8ZXDBg8jsA8KqWnfraqm8dcMkreEc2DDpZAYfuPdkckGPyMaW_AIyJRh6RI86XRHUbMlzSNJRgw_RAHT4Hi9TPkUZ8jLMrNvTDCx3RpBJXIm-RphxxeshbOntqcjZ2O-KU18qWIZeIjlochkTzvPIh0lT6RWQyfiIfvBkSnu7jCbn_eX139bu6uf315-ryprKCqVw5zoVFI0Qt6-UOga01PWLtVQOWcXBe1r1TRiA6g96zRvBeSt5KbljtGn5CzndzlyOeCqasx5DWpcyEc0m6ZQxAdOq_IBMtiIbXCyh2oI1zShG9foxhNPFFM9DrN_RqtV6t1q3Sr9_QsMi-7OeXfkR3EO3tX_pn-75J1gw-msmGdMCUbLuardiPHYaLac8Bo0424GTRhYg2azeHt_f4B_Wfp2Y</recordid><startdate>19870701</startdate><enddate>19870701</enddate><creator>Dudman, Nicholas P.B.</creator><creator>Hicks, Christine</creator><creator>Wilcken, David E.L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19870701</creationdate><title>A sluicing device for reproducibly measuring the strength of attachment of cultured cells to their substrate</title><author>Dudman, Nicholas P.B. ; Hicks, Christine ; Wilcken, David E.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d334cea442623094e8cabee2f750c130df62bd7a4eedaeff1543b663863a12d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animal cells</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>blood vessels</topic><topic>Cell Adhesion</topic><topic>Cell Count</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>cell density</topic><topic>cell detachment</topic><topic>Cell hybridization, cell fusion, hybridomas</topic><topic>Cells, Cultured</topic><topic>controlled sluicing</topic><topic>Cytological Techniques - instrumentation</topic><topic>endothelial cells</topic><topic>endothelium</topic><topic>Endothelium - cytology</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrostatic Pressure</topic><topic>man</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Sodium Chloride</topic><topic>Solutions</topic><topic>Trypsin - pharmacology</topic><topic>trypsin effects</topic><topic>Vacuum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dudman, Nicholas P.B.</creatorcontrib><creatorcontrib>Hicks, Christine</creatorcontrib><creatorcontrib>Wilcken, David E.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dudman, Nicholas P.B.</au><au>Hicks, Christine</au><au>Wilcken, David E.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sluicing device for reproducibly measuring the strength of attachment of cultured cells to their substrate</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1987-07-01</date><risdate>1987</risdate><volume>164</volume><issue>1</issue><spage>35</spage><epage>38</epage><pages>35-38</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>We assessed the strength of attachment of cultured human vascular endothelial cells to tissue culture plastic by controlled sluicing of cells, grown on multiwell plates, with isotonic saline using a specially designed nozzle attached to a reciprocating pump. Cells which were not dislodged were trypsinized and counted. The percentage of cells dislodged was proportional to the pump speed and to the volume of saline pumped. For given pump settings the proportion of cells detached was reproducible. The percentage of cells detached by sluicing increased at lower cell densities and decreased when the duration of trypsinizing of cells to be plated in multiwells, prior to sluicing, was increased. Our system is suitable for evaluating effects of biochemical interventions on the strength of cell attachment to the substrate.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3314579</pmid><doi>10.1016/0003-2697(87)90363-0</doi><tpages>4</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1987-07, Vol.164 (1), p.35-38 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animal cells Biological and medical sciences Biotechnology blood vessels Cell Adhesion Cell Count Cell cultures. Hybridization. Fusion cell density cell detachment Cell hybridization, cell fusion, hybridomas Cells, Cultured controlled sluicing Cytological Techniques - instrumentation endothelial cells endothelium Endothelium - cytology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Humans Hydrostatic Pressure man Methods. Procedures. Technologies Molecular and cellular biology Sodium Chloride Solutions Trypsin - pharmacology trypsin effects Vacuum |
| title | A sluicing device for reproducibly measuring the strength of attachment of cultured cells to their substrate |
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