Isolation and structural studies of the intact scrapie agent protein

Purification of the scrapie agent by methods using digestion with proteinase K yields a protein product, PrP-27–30, with an apparent mass of 27–30 kDa (D. C. Bolton et al. (1982) Science 218, 1309–1311; S. B. Prusiner et al. (1982) Biochemistry 21, 6942–6950). In contrast, a 33–37 kDa glycoprotein,...

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