Measurement of branched-chain ketoacids in plasma by high-performance liquid chromatography

Branched-chain ketoacids were isolated from plasma or serum samples by acidification, passage through a cationic exchange resin, ether extraction, and extraction of the ether layer with phosphate buffer. The recovery of 2-[1- 14C]ketoisocaproate taken through these procedures averaged 95 ± 3%. Branc...

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Veröffentlicht in:Analytical biochemistry 1987-08, Vol.164 (2), p.287-291
Hauptverfasser: Walser, Mackenzie, Swain, Louis M., Alexander, Valerie
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Sprache:eng
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