Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products

An RNA-dependent RNA polymerase activity of infectious bursal disease virus (IBDV) could be demonstrated without any special treatment of the virus particles. Ca 2+ ions had to be removed from the reaction mixture. Mg 2+ (4 mM) was essential for the polymerase activity which was optimal at pH 8.5 an...

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Veröffentlicht in:	Virus research 1987-08, Vol.8 (2), p.127-140
Hauptverfasser:	Spies, U., Müller, H., Becht, H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Birnaviridae Centrifugation, Density Gradient Chick Embryo Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Infectious bursal disease virus Infectious bursal disease virus - enzymology Infectious bursal disease virus - genetics Microbiology Morphology, structure, chemical composition, physicochemical properties Nucleic Acid Hybridization Polymerase activity Protein Biosynthesis Reoviridae - enzymology RNA Nucleotidyltransferases - metabolism RNA Replicase - analysis RNA Replicase - antagonists & inhibitors RNA Replicase - metabolism RNA, Double-Stranded - analysis RNA, Double-Stranded - biosynthesis RNA, Double-Stranded - genetics RNA, Viral - analysis RNA, Viral - biosynthesis RNA, Viral - genetics Sequence Homology, Nucleic Acid Transcription, Genetic Virology
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