Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei
Mitochondrial membranes from Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation ( K i = 16 μM) with the same affinity as it stimulated NADH-fumarate reductase activit...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1987-08, Vol.25 (1), p.55-60 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Turrens, Julio F. |
| description | Mitochondrial membranes from
Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation (
K
i = 16
μM) with the same affinity as it stimulated NADH-fumarate reductase activity. Superoxide anion production was also 65% inhibited by fumarate (
K
i = 20
μM). The
K
M for NADH of the NADH-fumarate reductase (60 μM) was also similar to that for hydrogen peroxide generation in the absence of fumarate (30 μM). These results suggest that the NADH-fumarate reductase is involved as a source for free radical generation in
T. brucei mitochondria. |
| doi_str_mv | 10.1016/0166-6851(87)90018-1 |
| format | Article |
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Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation (
K
i = 16
μM) with the same affinity as it stimulated NADH-fumarate reductase activity. Superoxide anion production was also 65% inhibited by fumarate (
K
i = 20
μM). The
K
M for NADH of the NADH-fumarate reductase (60 μM) was also similar to that for hydrogen peroxide generation in the absence of fumarate (30 μM). These results suggest that the NADH-fumarate reductase is involved as a source for free radical generation in
T. brucei mitochondria.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(87)90018-1</identifier><identifier>PMID: 2823135</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; anions ; Biological and medical sciences ; Cytochrome c Group - metabolism ; Free radicals ; Fumarate reductase ; Fumarates - metabolism ; Fundamental and applied biological sciences. Psychology ; Human protozoal diseases ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Infectious diseases ; Life cycle. Host-agent relationship. Pathogenesis ; Medical sciences ; metabolism ; mitochondria ; NAD (coenzyme) ; NAD - metabolism ; Oxidation-Reduction ; Oxidoreductases - metabolism ; Oxidoreductases Acting on CH-CH Group Donors ; Oxygen Consumption ; Parasitic diseases ; Protozoa ; Protozoal diseases ; Superoxide anion ; Superoxides - metabolism ; Tropical medicine ; Trypanosoma brucei ; Trypanosoma brucei brucei - enzymology ; Trypanosoma brucei brucei - metabolism ; Trypanosome ; Trypanosomiasis</subject><ispartof>Molecular and biochemical parasitology, 1987-08, Vol.25 (1), p.55-60</ispartof><rights>1987</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-49b805ad88d83b0a9508d528f221601d9b801321feab5b6e47e04164b291640f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0166685187900181$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8323614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2823135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turrens, Julio F.</creatorcontrib><title>Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>Mitochondrial membranes from
Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation (
K
i = 16
μM) with the same affinity as it stimulated NADH-fumarate reductase activity. Superoxide anion production was also 65% inhibited by fumarate (
K
i = 20
μM). The
K
M for NADH of the NADH-fumarate reductase (60 μM) was also similar to that for hydrogen peroxide generation in the absence of fumarate (30 μM). These results suggest that the NADH-fumarate reductase is involved as a source for free radical generation in
T. brucei mitochondria.</description><subject>Animals</subject><subject>anions</subject><subject>Biological and medical sciences</subject><subject>Cytochrome c Group - metabolism</subject><subject>Free radicals</subject><subject>Fumarate reductase</subject><subject>Fumarates - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human protozoal diseases</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Infectious diseases</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Medical sciences</subject><subject>metabolism</subject><subject>mitochondria</subject><subject>NAD (coenzyme)</subject><subject>NAD - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - metabolism</subject><subject>Oxidoreductases Acting on CH-CH Group Donors</subject><subject>Oxygen Consumption</subject><subject>Parasitic diseases</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Superoxide anion</subject><subject>Superoxides - metabolism</subject><subject>Tropical medicine</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - enzymology</subject><subject>Trypanosoma brucei brucei - metabolism</subject><subject>Trypanosome</subject><subject>Trypanosomiasis</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCNwCRA0JwCHgcx5lckKryp0gVINGeLceetEbZeGsn0P32OOxqj-3Bz4f3e6PRG8ZeAH8PHNSH_FSpsIa32LxrOQcs4RFbATaibKXAx2x1QJ6wpyn95pzXjVLH7FigqKCqV-zvz5CS7wYqYsgS-mK6oeL76afzsp_XJpopO-RmO5lEhR-LNG8ohjvvqDCjD2NWV9xsXQzXNBYHbxPDElqAHLqM240ZQwprU3RxtuSfsaPeDIme7_8TdvXl8-XZeXnx4-u3s9OL0koJUynbDnltHKLDquOmrTm6WmAvBCgObrGhEtCT6epOkWyIS1CyE21W3lcn7M1ubl7odqY06bVPlobBjBTmpBF4y5VqHgRBYo2IKoNyB9qYq4vU6030uamtBq6Xw-ildb20rrHR_w-jIcde7ufP3ZrcIbS_RPZf732TrBn6aEbr0wHDSlQKZMZe7bDeBG2uY0aufoncARdciQbbTHzcEZRr_eMp6mQ9jZacj2Qn7YK_f9N_ELCy2g</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>Turrens, Julio F.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19870801</creationdate><title>Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei</title><author>Turrens, Julio F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-49b805ad88d83b0a9508d528f221601d9b801321feab5b6e47e04164b291640f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>anions</topic><topic>Biological and medical sciences</topic><topic>Cytochrome c Group - metabolism</topic><topic>Free radicals</topic><topic>Fumarate reductase</topic><topic>Fumarates - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human protozoal diseases</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Infectious diseases</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Medical sciences</topic><topic>metabolism</topic><topic>mitochondria</topic><topic>NAD (coenzyme)</topic><topic>NAD - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - metabolism</topic><topic>Oxidoreductases Acting on CH-CH Group Donors</topic><topic>Oxygen Consumption</topic><topic>Parasitic diseases</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Superoxide anion</topic><topic>Superoxides - metabolism</topic><topic>Tropical medicine</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - enzymology</topic><topic>Trypanosoma brucei brucei - metabolism</topic><topic>Trypanosome</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turrens, Julio F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turrens, Julio F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>25</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>Mitochondrial membranes from
Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation (
K
i = 16
μM) with the same affinity as it stimulated NADH-fumarate reductase activity. Superoxide anion production was also 65% inhibited by fumarate (
K
i = 20
μM). The
K
M for NADH of the NADH-fumarate reductase (60 μM) was also similar to that for hydrogen peroxide generation in the absence of fumarate (30 μM). These results suggest that the NADH-fumarate reductase is involved as a source for free radical generation in
T. brucei mitochondria.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2823135</pmid><doi>10.1016/0166-6851(87)90018-1</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1987-08, Vol.25 (1), p.55-60 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81090667 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals anions Biological and medical sciences Cytochrome c Group - metabolism Free radicals Fumarate reductase Fumarates - metabolism Fundamental and applied biological sciences. Psychology Human protozoal diseases Hydrogen peroxide Hydrogen Peroxide - metabolism Infectious diseases Life cycle. Host-agent relationship. Pathogenesis Medical sciences metabolism mitochondria NAD (coenzyme) NAD - metabolism Oxidation-Reduction Oxidoreductases - metabolism Oxidoreductases Acting on CH-CH Group Donors Oxygen Consumption Parasitic diseases Protozoa Protozoal diseases Superoxide anion Superoxides - metabolism Tropical medicine Trypanosoma brucei Trypanosoma brucei brucei - enzymology Trypanosoma brucei brucei - metabolism Trypanosome Trypanosomiasis |
| title | Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei |
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