GH binding to liver in young and old female rats: relation to somatomedin-C secretion
Age-related changes in binding of 125I-bovine GH to liver membrane fractions were measured in female Long-Evans rats 2, 6, 12 and 20 months of age. Specific GH binding did not change between 2 and 6 months of age but increased significantly at 12 and 20 months of age. Scatchard analysis showed that...
Gespeichert in:
| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1987-11, Vol.186 (2), p.229-233 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 233 |
|---|---|
| container_issue | 2 |
| container_start_page | 229 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 186 |
| creator | TAKAHASHI, S MEITES, J |
| description | Age-related changes in binding of 125I-bovine GH to liver membrane fractions were measured in female Long-Evans rats 2, 6, 12 and 20 months of age. Specific GH binding did not change between 2 and 6 months of age but increased significantly at 12 and 20 months of age. Scatchard analysis showed that the plots were curvilinear and consisted of high- and low-affinity binding sites. The age-related increases in binding sites were mainly due to an increase in number of low-affinity binding sites. Serum somatomedin-C (SM-C) levels in 20-month-old rats were about half those in the 6-month-old rats. Twice daily injections of ovine GH (2 mg/kg body wt) for 7 days depressed liver GH binding and increased serum SM-C levels in 19-month-old female rats, but had no effect on GH binding in 2-month-old female rats. These results suggest that the increase in liver GH binding sites and the decrease in SM-C secretion are associated with our previously reported decrease in GH secretion in old female rats. |
| doi_str_mv | 10.3181/00379727-186-42608 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_81089623</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81089623</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-9fb513b37d543dc1285363f02ed382fed7ed04b18cdda59a77ede492dff498d33</originalsourceid><addsrcrecordid>eNo9kE1LHTEYhUNR9Nb2DxSEINLd1HxMJpnu5NJqQXCj65BJ3tSUmcQmuQX_vRnvravw5jznLB6EvlDyjVNFrwjhcpRMdlQNXc8Goj6gDRVMdJRLfoQ2K9CtxCn6WMofQshAGDlBJ3yQlA90gx5vbvEUogvxN64Jz-EfZBwifkm79mOiw2l22MNiZsDZ1PIdZ5hNDSmufEmLqWmB1u-2uIDNsEaf0LE3c4HPh_cMPf788bC97e7ub35tr-86y0Vfu9FPgvKJSyd67ixlSvCBe8LAccU8OAmO9BNV1jkjRiPbDf3InPf9qBznZ-hiv5tKDbrYUME-2RQj2KoFV1SIsUFf99BzTn93UKpeQrEwzyZC2hWtKFHjwNY1tgdtTqVk8Po5h8XkF02JXoXr_8J1E67fhLfS-WF9NzUP75WD4ZZfHnJTrJl9NtGG8o5J2UZkz18BwhWHMA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81089623</pqid></control><display><type>article</type><title>GH binding to liver in young and old female rats: relation to somatomedin-C secretion</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>TAKAHASHI, S ; MEITES, J</creator><creatorcontrib>TAKAHASHI, S ; MEITES, J ; Michigan State Univ., East Lansing (USA)</creatorcontrib><description>Age-related changes in binding of 125I-bovine GH to liver membrane fractions were measured in female Long-Evans rats 2, 6, 12 and 20 months of age. Specific GH binding did not change between 2 and 6 months of age but increased significantly at 12 and 20 months of age. Scatchard analysis showed that the plots were curvilinear and consisted of high- and low-affinity binding sites. The age-related increases in binding sites were mainly due to an increase in number of low-affinity binding sites. Serum somatomedin-C (SM-C) levels in 20-month-old rats were about half those in the 6-month-old rats. Twice daily injections of ovine GH (2 mg/kg body wt) for 7 days depressed liver GH binding and increased serum SM-C levels in 19-month-old female rats, but had no effect on GH binding in 2-month-old female rats. These results suggest that the increase in liver GH binding sites and the decrease in SM-C secretion are associated with our previously reported decrease in GH secretion in old female rats.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1525-1373</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-186-42608</identifier><identifier>PMID: 3671361</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>Malden, MA: Blackwell Science</publisher><subject>550201 - Biochemistry- Tracer Techniques ; AGE DEPENDENCE ; Aging ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; BODY ; CELL CONSTITUENTS ; CELL MEMBRANES ; DAYS LIVING RADIOISOTOPES ; DIGESTIVE SYSTEM ; ELECTRON CAPTURE RADIOISOTOPES ; Female ; Fundamental and applied biological sciences. Psychology ; GLANDS ; Growth Hormone - metabolism ; HORMONES ; Insulin-Like Growth Factor I - metabolism ; INTERMEDIATE MASS NUCLEI ; IODINE 125 ; IODINE ISOTOPES ; ISOTOPE APPLICATIONS ; ISOTOPES ; KINETICS ; LIVER ; Liver - metabolism ; Liver. Bile. Biliary tracts ; MAMMALS ; MEMBRANE PROTEINS ; MEMBRANES ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; ORGANS ; PEPTIDE HORMONES ; PITUITARY HORMONES ; PROTEINS ; RADIOISOTOPES ; RATS ; Rats, Inbred Strains ; REACTION KINETICS ; RECEPTORS ; Receptors, Somatotropin - metabolism ; RODENTS ; Somatomedins - metabolism ; STH ; TRACER TECHNIQUES ; VERTEBRATES ; Vertebrates: digestive system</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1987-11, Vol.186 (2), p.229-233</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-9fb513b37d543dc1285363f02ed382fed7ed04b18cdda59a77ede492dff498d33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7760874$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3671361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5381559$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>TAKAHASHI, S</creatorcontrib><creatorcontrib>MEITES, J</creatorcontrib><creatorcontrib>Michigan State Univ., East Lansing (USA)</creatorcontrib><title>GH binding to liver in young and old female rats: relation to somatomedin-C secretion</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Age-related changes in binding of 125I-bovine GH to liver membrane fractions were measured in female Long-Evans rats 2, 6, 12 and 20 months of age. Specific GH binding did not change between 2 and 6 months of age but increased significantly at 12 and 20 months of age. Scatchard analysis showed that the plots were curvilinear and consisted of high- and low-affinity binding sites. The age-related increases in binding sites were mainly due to an increase in number of low-affinity binding sites. Serum somatomedin-C (SM-C) levels in 20-month-old rats were about half those in the 6-month-old rats. Twice daily injections of ovine GH (2 mg/kg body wt) for 7 days depressed liver GH binding and increased serum SM-C levels in 19-month-old female rats, but had no effect on GH binding in 2-month-old female rats. These results suggest that the increase in liver GH binding sites and the decrease in SM-C secretion are associated with our previously reported decrease in GH secretion in old female rats.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>AGE DEPENDENCE</subject><subject>Aging</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>BODY</subject><subject>CELL CONSTITUENTS</subject><subject>CELL MEMBRANES</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DIGESTIVE SYSTEM</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLANDS</subject><subject>Growth Hormone - metabolism</subject><subject>HORMONES</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>IODINE 125</subject><subject>IODINE ISOTOPES</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>KINETICS</subject><subject>LIVER</subject><subject>Liver - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>MAMMALS</subject><subject>MEMBRANE PROTEINS</subject><subject>MEMBRANES</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PEPTIDE HORMONES</subject><subject>PITUITARY HORMONES</subject><subject>PROTEINS</subject><subject>RADIOISOTOPES</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>REACTION KINETICS</subject><subject>RECEPTORS</subject><subject>Receptors, Somatotropin - metabolism</subject><subject>RODENTS</subject><subject>Somatomedins - metabolism</subject><subject>STH</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES</subject><subject>Vertebrates: digestive system</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1525-1373</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LHTEYhUNR9Nb2DxSEINLd1HxMJpnu5NJqQXCj65BJ3tSUmcQmuQX_vRnvravw5jznLB6EvlDyjVNFrwjhcpRMdlQNXc8Goj6gDRVMdJRLfoQ2K9CtxCn6WMofQshAGDlBJ3yQlA90gx5vbvEUogvxN64Jz-EfZBwifkm79mOiw2l22MNiZsDZ1PIdZ5hNDSmufEmLqWmB1u-2uIDNsEaf0LE3c4HPh_cMPf788bC97e7ub35tr-86y0Vfu9FPgvKJSyd67ixlSvCBe8LAccU8OAmO9BNV1jkjRiPbDf3InPf9qBznZ-hiv5tKDbrYUME-2RQj2KoFV1SIsUFf99BzTn93UKpeQrEwzyZC2hWtKFHjwNY1tgdtTqVk8Po5h8XkF02JXoXr_8J1E67fhLfS-WF9NzUP75WD4ZZfHnJTrJl9NtGG8o5J2UZkz18BwhWHMA</recordid><startdate>19871101</startdate><enddate>19871101</enddate><creator>TAKAHASHI, S</creator><creator>MEITES, J</creator><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19871101</creationdate><title>GH binding to liver in young and old female rats: relation to somatomedin-C secretion</title><author>TAKAHASHI, S ; MEITES, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-9fb513b37d543dc1285363f02ed382fed7ed04b18cdda59a77ede492dff498d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>AGE DEPENDENCE</topic><topic>Aging</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>BODY</topic><topic>CELL CONSTITUENTS</topic><topic>CELL MEMBRANES</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DIGESTIVE SYSTEM</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLANDS</topic><topic>Growth Hormone - metabolism</topic><topic>HORMONES</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>IODINE 125</topic><topic>IODINE ISOTOPES</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>KINETICS</topic><topic>LIVER</topic><topic>Liver - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>MAMMALS</topic><topic>MEMBRANE PROTEINS</topic><topic>MEMBRANES</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PEPTIDE HORMONES</topic><topic>PITUITARY HORMONES</topic><topic>PROTEINS</topic><topic>RADIOISOTOPES</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>REACTION KINETICS</topic><topic>RECEPTORS</topic><topic>Receptors, Somatotropin - metabolism</topic><topic>RODENTS</topic><topic>Somatomedins - metabolism</topic><topic>STH</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKAHASHI, S</creatorcontrib><creatorcontrib>MEITES, J</creatorcontrib><creatorcontrib>Michigan State Univ., East Lansing (USA)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKAHASHI, S</au><au>MEITES, J</au><aucorp>Michigan State Univ., East Lansing (USA)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GH binding to liver in young and old female rats: relation to somatomedin-C secretion</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1987-11-01</date><risdate>1987</risdate><volume>186</volume><issue>2</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1525-1373</eissn><eissn>1535-3699</eissn><coden>PSEBAA</coden><abstract>Age-related changes in binding of 125I-bovine GH to liver membrane fractions were measured in female Long-Evans rats 2, 6, 12 and 20 months of age. Specific GH binding did not change between 2 and 6 months of age but increased significantly at 12 and 20 months of age. Scatchard analysis showed that the plots were curvilinear and consisted of high- and low-affinity binding sites. The age-related increases in binding sites were mainly due to an increase in number of low-affinity binding sites. Serum somatomedin-C (SM-C) levels in 20-month-old rats were about half those in the 6-month-old rats. Twice daily injections of ovine GH (2 mg/kg body wt) for 7 days depressed liver GH binding and increased serum SM-C levels in 19-month-old female rats, but had no effect on GH binding in 2-month-old female rats. These results suggest that the increase in liver GH binding sites and the decrease in SM-C secretion are associated with our previously reported decrease in GH secretion in old female rats.</abstract><cop>Malden, MA</cop><pub>Blackwell Science</pub><pmid>3671361</pmid><doi>10.3181/00379727-186-42608</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1987-11, Vol.186 (2), p.229-233 |
| issn | 0037-9727 1535-3702 1525-1373 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81089623 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 550201 - Biochemistry- Tracer Techniques AGE DEPENDENCE Aging ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BODY CELL CONSTITUENTS CELL MEMBRANES DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM ELECTRON CAPTURE RADIOISOTOPES Female Fundamental and applied biological sciences. Psychology GLANDS Growth Hormone - metabolism HORMONES Insulin-Like Growth Factor I - metabolism INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES ISOTOPE APPLICATIONS ISOTOPES KINETICS LIVER Liver - metabolism Liver. Bile. Biliary tracts MAMMALS MEMBRANE PROTEINS MEMBRANES NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PEPTIDE HORMONES PITUITARY HORMONES PROTEINS RADIOISOTOPES RATS Rats, Inbred Strains REACTION KINETICS RECEPTORS Receptors, Somatotropin - metabolism RODENTS Somatomedins - metabolism STH TRACER TECHNIQUES VERTEBRATES Vertebrates: digestive system |
| title | GH binding to liver in young and old female rats: relation to somatomedin-C secretion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T14%3A01%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GH%20binding%20to%20liver%20in%20young%20and%20old%20female%20rats:%20relation%20to%20somatomedin-C%20secretion&rft.jtitle=Experimental%20biology%20and%20medicine%20(Maywood,%20N.J.)&rft.au=TAKAHASHI,%20S&rft.aucorp=Michigan%20State%20Univ.,%20East%20Lansing%20(USA)&rft.date=1987-11-01&rft.volume=186&rft.issue=2&rft.spage=229&rft.epage=233&rft.pages=229-233&rft.issn=0037-9727&rft.eissn=1525-1373&rft.coden=PSEBAA&rft_id=info:doi/10.3181/00379727-186-42608&rft_dat=%3Cproquest_osti_%3E81089623%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81089623&rft_id=info:pmid/3671361&rfr_iscdi=true |