Particulate cyclic 3′,5′-nucleotide phosphodiesterase and calmodulin of cardiac muscle
1. 1. Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle. 2. 2. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific. 3. 3....
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| Veröffentlicht in: | International journal of biochemistry 1984, Vol.16 (5), p.483-488 |
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| container_end_page | 488 |
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| container_issue | 5 |
| container_start_page | 483 |
| container_title | International journal of biochemistry |
| container_volume | 16 |
| creator | Ahluwalia, Gurpreet Rhoads, Allen R. Lulla, Madhu |
| description | 1.
1. Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle.
2.
2. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific.
3.
3. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl
2. |
| doi_str_mv | 10.1016/0020-711X(84)90164-2 |
| format | Article |
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1. Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle.
2.
2. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific.
3.
3. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl
2.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(84)90164-2</identifier><identifier>PMID: 6327416</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3',5'-Cyclic-AMP Phosphodiesterases - analysis ; 3',5'-Cyclic-GMP Phosphodiesterases - analysis ; Animals ; Calmodulin - analysis ; Cattle ; Cell Nucleus - analysis ; Cyclic AMP - analysis ; Egtazic Acid - pharmacology ; Magnesium - pharmacology ; Magnesium Chloride ; Myocardium - analysis ; Myocardium - enzymology ; Potassium Chloride - pharmacology</subject><ispartof>International journal of biochemistry, 1984, Vol.16 (5), p.483-488</ispartof><rights>1984</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-5591765f3aa8843ea1ab3e0076b4532f56a9b9a50ae62dfad781a381f06cbf633</citedby><cites>FETCH-LOGICAL-c357t-5591765f3aa8843ea1ab3e0076b4532f56a9b9a50ae62dfad781a381f06cbf633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6327416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahluwalia, Gurpreet</creatorcontrib><creatorcontrib>Rhoads, Allen R.</creatorcontrib><creatorcontrib>Lulla, Madhu</creatorcontrib><title>Particulate cyclic 3′,5′-nucleotide phosphodiesterase and calmodulin of cardiac muscle</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle.
2.
2. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific.
3.
3. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl
2.</description><subject>3',5'-Cyclic-AMP Phosphodiesterases - analysis</subject><subject>3',5'-Cyclic-GMP Phosphodiesterases - analysis</subject><subject>Animals</subject><subject>Calmodulin - analysis</subject><subject>Cattle</subject><subject>Cell Nucleus - analysis</subject><subject>Cyclic AMP - analysis</subject><subject>Egtazic Acid - pharmacology</subject><subject>Magnesium - pharmacology</subject><subject>Magnesium Chloride</subject><subject>Myocardium - analysis</subject><subject>Myocardium - enzymology</subject><subject>Potassium Chloride - pharmacology</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhrNQdBx9A4WuRMFq0rRJuxFEvIGgCwVxE06TE4z0MiatMDufyUfyScw4wyxdnBzO5f-TfITsM3rKKBNnlGY0lYy9HJX5cRU7eZptkMm6vU12QninlFVlzrbIluCZzJmYkNdH8IPTYwMDJnquG6cT_vP1fVLEI-1G3WA_OIPJ7K0PMYzDMKCHgAl0JtHQtL0ZG9clvY2VNw500o4h6nbJpoUm4N4qT8nz9dXT5W16_3Bzd3lxn2peyCEtiopJUVgOUJY5R2BQc6RUijoveGYLAVVdQUEBRWYsGFky4CWzVOjaCs6n5HDpO_P9xxifp1oXNDYNdNiPQZWMSskrFhfz5aL2fQgerZp514KfK0bVAqNa8FILXqrM1R9GlUXZwcp_rFs0a9GKYZyfL-cYP_np0KugHXYajfOoB2V69_8FvwRNhko</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Ahluwalia, Gurpreet</creator><creator>Rhoads, Allen R.</creator><creator>Lulla, Madhu</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1984</creationdate><title>Particulate cyclic 3′,5′-nucleotide phosphodiesterase and calmodulin of cardiac muscle</title><author>Ahluwalia, Gurpreet ; Rhoads, Allen R. ; Lulla, Madhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-5591765f3aa8843ea1ab3e0076b4532f56a9b9a50ae62dfad781a381f06cbf633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>3',5'-Cyclic-AMP Phosphodiesterases - analysis</topic><topic>3',5'-Cyclic-GMP Phosphodiesterases - analysis</topic><topic>Animals</topic><topic>Calmodulin - analysis</topic><topic>Cattle</topic><topic>Cell Nucleus - analysis</topic><topic>Cyclic AMP - analysis</topic><topic>Egtazic Acid - pharmacology</topic><topic>Magnesium - pharmacology</topic><topic>Magnesium Chloride</topic><topic>Myocardium - analysis</topic><topic>Myocardium - enzymology</topic><topic>Potassium Chloride - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Ahluwalia, Gurpreet</creatorcontrib><creatorcontrib>Rhoads, Allen R.</creatorcontrib><creatorcontrib>Lulla, Madhu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahluwalia, Gurpreet</au><au>Rhoads, Allen R.</au><au>Lulla, Madhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Particulate cyclic 3′,5′-nucleotide phosphodiesterase and calmodulin of cardiac muscle</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1984</date><risdate>1984</risdate><volume>16</volume><issue>5</issue><spage>483</spage><epage>488</epage><pages>483-488</pages><issn>0020-711X</issn><abstract>1.
1. Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle.
2.
2. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific.
3.
3. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl
2.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>6327416</pmid><doi>10.1016/0020-711X(84)90164-2</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0020-711X |
| ispartof | International journal of biochemistry, 1984, Vol.16 (5), p.483-488 |
| issn | 0020-711X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 3',5'-Cyclic-AMP Phosphodiesterases - analysis 3',5'-Cyclic-GMP Phosphodiesterases - analysis Animals Calmodulin - analysis Cattle Cell Nucleus - analysis Cyclic AMP - analysis Egtazic Acid - pharmacology Magnesium - pharmacology Magnesium Chloride Myocardium - analysis Myocardium - enzymology Potassium Chloride - pharmacology |
| title | Particulate cyclic 3′,5′-nucleotide phosphodiesterase and calmodulin of cardiac muscle |
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