Purification, kinetic behavior, and regulation of NAD(P)+ malic enzyme of tumor mitochondria

The purification and kinetic characterization of an NAD(P)+-malic enzyme from 22aH mouse hepatoma mitochondria are described. The enzyme was purified 328-fold with a final yield of 51% and specific activity of 38.1 units/mg of protein by employing DEAE-cellulose chromatography and an ATP affinity co...

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Veröffentlicht in:	The Journal of biological chemistry 1984-05, Vol.259 (10), p.6222-6227
Hauptverfasser:	Moreadith, R W, Lehninger, A L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Carcinoma, Ehrlich Tumor - enzymology Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Female Fundamental and applied biological sciences. Psychology Kinetics Leukemia, Experimental - enzymology Liver Neoplasms, Experimental Malate Dehydrogenase - isolation & purification Malate Dehydrogenase - metabolism Male Mammary Neoplasms, Experimental - enzymology Mice Mice, Inbred Strains Mitochondria - enzymology Mitochondria, Liver - enzymology Molecular and cellular biology Molecular Weight NAD - metabolism NADP - metabolism Neoplasms, Experimental - enzymology Rats Thymoma - enzymology Thymus Neoplasms - enzymology
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