Structures of Oligosaccharides on β-Galactosidase from Aspergillus oryzae
The carbohydrate portions of β-galactosidase from Aspergillus oryzae were found to be composed of two types of sugar chains. They were released equally well with endo-β-./V-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of th...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1987, Vol.102 (1), p.171-179 |
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| creator | NAKAO, Yoshihide KOZUTSUMI, Yasunori FUNAKOSHI, Ikuo KAWASAKI, Toshisuke YAMASHINA, Ikuo MUTSAERS, Johanna H.G.M. VAN HALBEEK, Herman VLIEGENTHART, Johannes F.G. |
| description | The carbohydrate portions of β-galactosidase from Aspergillus oryzae were found to be composed of two types of sugar chains. They were released equally well with endo-β-./V-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of the total carbohydrate chains, were composed of galactomannan-type oligosaccharides, which consisted of man-nose, galactose, glucose, and glucosamine in the molar ratios of 30.0, 16.4, 1.4, and 2.1 per mol of aspartic acid, respectively. The short sugar chains (fraction II), corresponding to 96% of the total carbohydrate chains, consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 9.4, 0.6, 0.3, and 1.7 per mol of aspartic acid, respectively. Both types of sugar chains were fractionated into neutral and acidic subfractions. The neutral subfraction of fraction I (I-N), corresponding to 1% of the total carbohydrate chains, was very heterogeneous in length and was resistant to digestion with a-mannosidase and β-galactosidase. The neutral subfraction of fraction II (II-N), corresponding to 91% of the total car-bohydrate, was composed of a mixture of oligosaccharides with oligomanneoside chains (MannGlcNAcol). The major components were similar to high mannose-type oligosaccharides of mammalian origin in their composition and size (n=5–9). However, digestion of II-N with al, 2-mannosidase produced considerable amounts of Man, GlcNAcol, an unusual product in the case of high mannose-type oligo-saccharides of mammalian origin, in addition to the common one, Man6GlcNAcol. The following structure is proposed for Man6GlcNAcol: |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a122030 |
| format | Article |
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They were released equally well with endo-β-./V-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of the total carbohydrate chains, were composed of galactomannan-type oligosaccharides, which consisted of man-nose, galactose, glucose, and glucosamine in the molar ratios of 30.0, 16.4, 1.4, and 2.1 per mol of aspartic acid, respectively. The short sugar chains (fraction II), corresponding to 96% of the total carbohydrate chains, consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 9.4, 0.6, 0.3, and 1.7 per mol of aspartic acid, respectively. Both types of sugar chains were fractionated into neutral and acidic subfractions. The neutral subfraction of fraction I (I-N), corresponding to 1% of the total carbohydrate chains, was very heterogeneous in length and was resistant to digestion with a-mannosidase and β-galactosidase. The neutral subfraction of fraction II (II-N), corresponding to 91% of the total car-bohydrate, was composed of a mixture of oligosaccharides with oligomanneoside chains (MannGlcNAcol). The major components were similar to high mannose-type oligosaccharides of mammalian origin in their composition and size (n=5–9). However, digestion of II-N with al, 2-mannosidase produced considerable amounts of Man, GlcNAcol, an unusual product in the case of high mannose-type oligo-saccharides of mammalian origin, in addition to the common one, Man6GlcNAcol. The following structure is proposed for Man6GlcNAcol:</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a122030</identifier><identifier>PMID: 3117779</identifier><identifier>CODEN: JOBIAO</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Aspergillus - enzymology ; Aspergillus oryzae ; Aspergillus oryzae - enzymology ; beta -galactosidase ; beta-Galactosidase - isolation & purification ; Biological and medical sciences ; Carbohydrate Sequence ; carbohydrates ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Galactosidases - isolation & purification ; Glycopeptides - isolation & purification ; Hydrolases ; Molecular Sequence Data ; oligosaccharides ; Oligosaccharides - isolation & purification</subject><ispartof>Journal of biochemistry (Tokyo), 1987, Vol.102 (1), p.171-179</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8387623$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3117779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAKAO, Yoshihide</creatorcontrib><creatorcontrib>KOZUTSUMI, Yasunori</creatorcontrib><creatorcontrib>FUNAKOSHI, Ikuo</creatorcontrib><creatorcontrib>KAWASAKI, Toshisuke</creatorcontrib><creatorcontrib>YAMASHINA, Ikuo</creatorcontrib><creatorcontrib>MUTSAERS, Johanna H.G.M.</creatorcontrib><creatorcontrib>VAN HALBEEK, Herman</creatorcontrib><creatorcontrib>VLIEGENTHART, Johannes F.G.</creatorcontrib><title>Structures of Oligosaccharides on β-Galactosidase from Aspergillus oryzae</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>The carbohydrate portions of β-galactosidase from Aspergillus oryzae were found to be composed of two types of sugar chains. They were released equally well with endo-β-./V-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of the total carbohydrate chains, were composed of galactomannan-type oligosaccharides, which consisted of man-nose, galactose, glucose, and glucosamine in the molar ratios of 30.0, 16.4, 1.4, and 2.1 per mol of aspartic acid, respectively. The short sugar chains (fraction II), corresponding to 96% of the total carbohydrate chains, consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 9.4, 0.6, 0.3, and 1.7 per mol of aspartic acid, respectively. Both types of sugar chains were fractionated into neutral and acidic subfractions. The neutral subfraction of fraction I (I-N), corresponding to 1% of the total carbohydrate chains, was very heterogeneous in length and was resistant to digestion with a-mannosidase and β-galactosidase. The neutral subfraction of fraction II (II-N), corresponding to 91% of the total car-bohydrate, was composed of a mixture of oligosaccharides with oligomanneoside chains (MannGlcNAcol). The major components were similar to high mannose-type oligosaccharides of mammalian origin in their composition and size (n=5–9). However, digestion of II-N with al, 2-mannosidase produced considerable amounts of Man, GlcNAcol, an unusual product in the case of high mannose-type oligo-saccharides of mammalian origin, in addition to the common one, Man6GlcNAcol. The following structure is proposed for Man6GlcNAcol:</description><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Aspergillus - enzymology</subject><subject>Aspergillus oryzae</subject><subject>Aspergillus oryzae - enzymology</subject><subject>beta -galactosidase</subject><subject>beta-Galactosidase - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Sequence</subject><subject>carbohydrates</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactosidases - isolation & purification</subject><subject>Glycopeptides - isolation & purification</subject><subject>Hydrolases</subject><subject>Molecular Sequence Data</subject><subject>oligosaccharides</subject><subject>Oligosaccharides - isolation & purification</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1OwzAQhS0EKqVwBKQsgF2K_5JJlggBBYFY8KOqm2ji2MUlqYudSMCxOAhnIoiKLaxG896nmacZQg4ZHTOai2P3apyvFq7zS6zDeFGqJ92MkXFOBd0gQwZJGvM0YZtkSClncc7ldJvshLD4brkQAzIQjAFAPiRXd63vVNt5HSJnotvazl1ApZ7Q2-pbW0afH_EF1qhaF2yFQUfGuyY6CSvt57auux7yb--od8mW6RPpvXUdkYfzs_vTSXx9e3F5enIdW0mzNpYlFVhyUBS5lJVGmrBKJpBJoY0CXlYlYFZCwkuqDaeSp6iN7FXDBQKKETn6mbvy7qXToS0aG5Sua1xq14UiYzQVUrI_QSZzCqnM_gFCLiCFHtxfg13Z6KpYedugfyvW5-z9g7WPQWFtPC6VDb9YJjJI-weMSPyD2dDq118b_XPRL4GkmExnxc0MxI2YPBZSfAE4jprz</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>NAKAO, Yoshihide</creator><creator>KOZUTSUMI, Yasunori</creator><creator>FUNAKOSHI, Ikuo</creator><creator>KAWASAKI, Toshisuke</creator><creator>YAMASHINA, Ikuo</creator><creator>MUTSAERS, Johanna H.G.M.</creator><creator>VAN HALBEEK, Herman</creator><creator>VLIEGENTHART, Johannes F.G.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Structures of Oligosaccharides on β-Galactosidase from Aspergillus oryzae</title><author>NAKAO, Yoshihide ; KOZUTSUMI, Yasunori ; FUNAKOSHI, Ikuo ; KAWASAKI, Toshisuke ; YAMASHINA, Ikuo ; MUTSAERS, Johanna H.G.M. ; VAN HALBEEK, Herman ; VLIEGENTHART, Johannes F.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i408t-4b03ab27c0a244dea051d457843efc72bdb7a8b752b0ef20426aef4db7f23a7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Aspergillus - enzymology</topic><topic>Aspergillus oryzae</topic><topic>Aspergillus oryzae - enzymology</topic><topic>beta -galactosidase</topic><topic>beta-Galactosidase - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Sequence</topic><topic>carbohydrates</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galactosidases - isolation & purification</topic><topic>Glycopeptides - isolation & purification</topic><topic>Hydrolases</topic><topic>Molecular Sequence Data</topic><topic>oligosaccharides</topic><topic>Oligosaccharides - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAKAO, Yoshihide</creatorcontrib><creatorcontrib>KOZUTSUMI, Yasunori</creatorcontrib><creatorcontrib>FUNAKOSHI, Ikuo</creatorcontrib><creatorcontrib>KAWASAKI, Toshisuke</creatorcontrib><creatorcontrib>YAMASHINA, Ikuo</creatorcontrib><creatorcontrib>MUTSAERS, Johanna H.G.M.</creatorcontrib><creatorcontrib>VAN HALBEEK, Herman</creatorcontrib><creatorcontrib>VLIEGENTHART, Johannes F.G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAKAO, Yoshihide</au><au>KOZUTSUMI, Yasunori</au><au>FUNAKOSHI, Ikuo</au><au>KAWASAKI, Toshisuke</au><au>YAMASHINA, Ikuo</au><au>MUTSAERS, Johanna H.G.M.</au><au>VAN HALBEEK, Herman</au><au>VLIEGENTHART, Johannes F.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structures of Oligosaccharides on β-Galactosidase from Aspergillus oryzae</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1987</date><risdate>1987</risdate><volume>102</volume><issue>1</issue><spage>171</spage><epage>179</epage><pages>171-179</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><coden>JOBIAO</coden><abstract>The carbohydrate portions of β-galactosidase from Aspergillus oryzae were found to be composed of two types of sugar chains. They were released equally well with endo-β-./V-acetylglucosaminidase H, but were distinct in their chain length. The long sugar chains (fraction I), corresponding to 4% of the total carbohydrate chains, were composed of galactomannan-type oligosaccharides, which consisted of man-nose, galactose, glucose, and glucosamine in the molar ratios of 30.0, 16.4, 1.4, and 2.1 per mol of aspartic acid, respectively. The short sugar chains (fraction II), corresponding to 96% of the total carbohydrate chains, consisted of mannose, galactose, glucose, and glucosamine in the molar ratios of 9.4, 0.6, 0.3, and 1.7 per mol of aspartic acid, respectively. Both types of sugar chains were fractionated into neutral and acidic subfractions. The neutral subfraction of fraction I (I-N), corresponding to 1% of the total carbohydrate chains, was very heterogeneous in length and was resistant to digestion with a-mannosidase and β-galactosidase. The neutral subfraction of fraction II (II-N), corresponding to 91% of the total car-bohydrate, was composed of a mixture of oligosaccharides with oligomanneoside chains (MannGlcNAcol). The major components were similar to high mannose-type oligosaccharides of mammalian origin in their composition and size (n=5–9). However, digestion of II-N with al, 2-mannosidase produced considerable amounts of Man, GlcNAcol, an unusual product in the case of high mannose-type oligo-saccharides of mammalian origin, in addition to the common one, Man6GlcNAcol. The following structure is proposed for Man6GlcNAcol:</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3117779</pmid><doi>10.1093/oxfordjournals.jbchem.a122030</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of biochemistry (Tokyo), 1987, Vol.102 (1), p.171-179 |
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| subjects | Amino Acids - analysis Analytical, structural and metabolic biochemistry Aspergillus - enzymology Aspergillus oryzae Aspergillus oryzae - enzymology beta -galactosidase beta-Galactosidase - isolation & purification Biological and medical sciences Carbohydrate Sequence carbohydrates Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Galactosidases - isolation & purification Glycopeptides - isolation & purification Hydrolases Molecular Sequence Data oligosaccharides Oligosaccharides - isolation & purification |
| title | Structures of Oligosaccharides on β-Galactosidase from Aspergillus oryzae |
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