Actions of 4-aminopyridine on mammalian ganglion cells
The effects of 4-aminopyridine (4-AP) on membrane electrical properties and synaptic transmission in neurons of the isolated rabbit superior cervical ganglion were investigated. 4-AP (0.03–0.1 mM) increased the amplitude of the fast excitatory postsynaptic potential (f-EPSP) without affecting apprec...
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| Veröffentlicht in: | Brain research 1984-04, Vol.298 (1), p.149-153 |
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| creator | Simmons, M.A. Dun, N.J. |
| description | The effects of 4-aminopyridine (4-AP) on membrane electrical properties and synaptic transmission in neurons of the isolated rabbit superior cervical ganglion were investigated. 4-AP (0.03–0.1 mM) increased the amplitude of the fast excitatory postsynaptic potential (f-EPSP) without affecting appreciably appreciably either the acetylcholine (ACh) depolarization induced by iontophoresis of ACh or the passive and active membrane properties of the neurons. At concentrations of 1–5 mM, 4-AP reversibly depressed the amplitude of the f-EPSP as well as the ACh depolarization; a slight to moderate prolongation of the action potential duration was observed. In addition to the effects on evoked synaptic potentials, 4-AP induced spontaneous discharges which were abolished reversibly by curare, low Ca solution or Co. The results indicate that 4-AP at low concentrations facilitated evoked as well as spontaneous release of ACh by a presynaptic mechanism, whereas at higher concentrations it exerted a curare-like effect on the postsynaptic membrane. |
| doi_str_mv | 10.1016/0006-8993(84)91159-4 |
| format | Article |
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At concentrations of 1–5 mM, 4-AP reversibly depressed the amplitude of the f-EPSP as well as the ACh depolarization; a slight to moderate prolongation of the action potential duration was observed. In addition to the effects on evoked synaptic potentials, 4-AP induced spontaneous discharges which were abolished reversibly by curare, low Ca solution or Co. The results indicate that 4-AP at low concentrations facilitated evoked as well as spontaneous release of ACh by a presynaptic mechanism, whereas at higher concentrations it exerted a curare-like effect on the postsynaptic membrane.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(84)91159-4</identifier><identifier>PMID: 6144356</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>4-Aminopyridine ; Aminopyridines - antagonists & inhibitors ; Aminopyridines - pharmacology ; Animals ; Biological and medical sciences ; Calcium - physiology ; curare-like effect ; Dose-Response Relationship, Drug ; Ganglia, Sympathetic - drug effects ; Hexamethonium ; Hexamethonium Compounds - pharmacology ; In Vitro Techniques ; Male ; Medical sciences ; Membrane Potentials - drug effects ; Miscellaneous ; Pharmacology. Drug treatments ; Rabbits ; spontaneous discharges ; synaptic transmission ; Synaptic Transmission - drug effects ; Tubocurarine - pharmacology</subject><ispartof>Brain research, 1984-04, Vol.298 (1), p.149-153</ispartof><rights>1984 Elsevier Science Publishers B.V.</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-7f66c9d8be2f5abb694f99e38d862975f5e045a34f43eaae718c013e8202eb313</citedby><cites>FETCH-LOGICAL-c417t-7f66c9d8be2f5abb694f99e38d862975f5e045a34f43eaae718c013e8202eb313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(84)91159-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8903387$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6144356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simmons, M.A.</creatorcontrib><creatorcontrib>Dun, N.J.</creatorcontrib><title>Actions of 4-aminopyridine on mammalian ganglion cells</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The effects of 4-aminopyridine (4-AP) on membrane electrical properties and synaptic transmission in neurons of the isolated rabbit superior cervical ganglion were investigated. 4-AP (0.03–0.1 mM) increased the amplitude of the fast excitatory postsynaptic potential (f-EPSP) without affecting appreciably appreciably either the acetylcholine (ACh) depolarization induced by iontophoresis of ACh or the passive and active membrane properties of the neurons. At concentrations of 1–5 mM, 4-AP reversibly depressed the amplitude of the f-EPSP as well as the ACh depolarization; a slight to moderate prolongation of the action potential duration was observed. In addition to the effects on evoked synaptic potentials, 4-AP induced spontaneous discharges which were abolished reversibly by curare, low Ca solution or Co. The results indicate that 4-AP at low concentrations facilitated evoked as well as spontaneous release of ACh by a presynaptic mechanism, whereas at higher concentrations it exerted a curare-like effect on the postsynaptic membrane.</description><subject>4-Aminopyridine</subject><subject>Aminopyridines - antagonists & inhibitors</subject><subject>Aminopyridines - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - physiology</subject><subject>curare-like effect</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ganglia, Sympathetic - drug effects</subject><subject>Hexamethonium</subject><subject>Hexamethonium Compounds - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Potentials - drug effects</subject><subject>Miscellaneous</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>spontaneous discharges</subject><subject>synaptic transmission</subject><subject>Synaptic Transmission - drug effects</subject><subject>Tubocurarine - pharmacology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo67r6DxR6ENFDNWnSNLkIy-IXLHjRc0jTiUTaZE26wv57W3fZo56G4X3mZXgQOif4lmDC7zDGPBdS0mvBbiQhpczZAZoSURU5Lxg-RNM9coxOUvocVkolnqAJJ4zRkk8Rn5veBZ-yYDOW6875sNpE1zgPWfBZp7tOt0777EP7j3YgMwNtm07RkdVtgrPdnKH3x4e3xXO-fH16WcyXuWGk6vPKcm5kI2oobKnrmktmpQQqGsELWZW2BMxKTZllFLSGigiDCQVR4AJqSugMXW17VzF8rSH1qnNp_EB7COukBMG8oBX7FyRUCMFIOYBsC5oYUopg1Sq6TseNIliNXtUoTY3SlGDq16sa-y92_eu6g2Z_tBM55Je7XCejWxu1Ny7tMSEH86IasPstBoO0bwdRJePAG2hcBNOrJri___gBVFCSNw</recordid><startdate>19840423</startdate><enddate>19840423</enddate><creator>Simmons, M.A.</creator><creator>Dun, N.J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19840423</creationdate><title>Actions of 4-aminopyridine on mammalian ganglion cells</title><author>Simmons, M.A. ; Dun, N.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-7f66c9d8be2f5abb694f99e38d862975f5e045a34f43eaae718c013e8202eb313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>4-Aminopyridine</topic><topic>Aminopyridines - antagonists & inhibitors</topic><topic>Aminopyridines - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>curare-like effect</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ganglia, Sympathetic - drug effects</topic><topic>Hexamethonium</topic><topic>Hexamethonium Compounds - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Potentials - drug effects</topic><topic>Miscellaneous</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>spontaneous discharges</topic><topic>synaptic transmission</topic><topic>Synaptic Transmission - drug effects</topic><topic>Tubocurarine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simmons, M.A.</creatorcontrib><creatorcontrib>Dun, N.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simmons, M.A.</au><au>Dun, N.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actions of 4-aminopyridine on mammalian ganglion cells</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1984-04-23</date><risdate>1984</risdate><volume>298</volume><issue>1</issue><spage>149</spage><epage>153</epage><pages>149-153</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The effects of 4-aminopyridine (4-AP) on membrane electrical properties and synaptic transmission in neurons of the isolated rabbit superior cervical ganglion were investigated. 4-AP (0.03–0.1 mM) increased the amplitude of the fast excitatory postsynaptic potential (f-EPSP) without affecting appreciably appreciably either the acetylcholine (ACh) depolarization induced by iontophoresis of ACh or the passive and active membrane properties of the neurons. At concentrations of 1–5 mM, 4-AP reversibly depressed the amplitude of the f-EPSP as well as the ACh depolarization; a slight to moderate prolongation of the action potential duration was observed. In addition to the effects on evoked synaptic potentials, 4-AP induced spontaneous discharges which were abolished reversibly by curare, low Ca solution or Co. The results indicate that 4-AP at low concentrations facilitated evoked as well as spontaneous release of ACh by a presynaptic mechanism, whereas at higher concentrations it exerted a curare-like effect on the postsynaptic membrane.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6144356</pmid><doi>10.1016/0006-8993(84)91159-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Brain research, 1984-04, Vol.298 (1), p.149-153 |
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| language | eng |
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| subjects | 4-Aminopyridine Aminopyridines - antagonists & inhibitors Aminopyridines - pharmacology Animals Biological and medical sciences Calcium - physiology curare-like effect Dose-Response Relationship, Drug Ganglia, Sympathetic - drug effects Hexamethonium Hexamethonium Compounds - pharmacology In Vitro Techniques Male Medical sciences Membrane Potentials - drug effects Miscellaneous Pharmacology. Drug treatments Rabbits spontaneous discharges synaptic transmission Synaptic Transmission - drug effects Tubocurarine - pharmacology |
| title | Actions of 4-aminopyridine on mammalian ganglion cells |
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