Isolation and structures of alligator gar ( Lepisosteus spatula) insulin and pancreatic polypeptide
Insulin and a 36-residue peptide with homology to pancreatic polypeptide (PP) were isolated from the endocrine pancreas of the alligator gar ( Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactiviti...
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| Veröffentlicht in: | General and comparative endocrinology 1987-09, Vol.67 (3), p.375-382 |
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| container_title | General and comparative endocrinology |
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| creator | Pollock, H.G. Kimmel, J.R. Hamilton, J.W. Rouse, J.B. Ebner, K.E. Lance, V. Rawitch, A.B. |
| description | Insulin and a 36-residue peptide with homology to pancreatic polypeptide (PP) were isolated from the endocrine pancreas of the alligator gar (
Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactivities during purification of the two peptides. The sequence of the 36-amino acid peptide containing a C-terminal tyrosinamide was identical at 31 of 36 positions to porcine neuropeptide Y (NPY). The amino acid sequence of this peptide is YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH
2. The second peptide, gar insulin, contains 52 amino acid residues and is composed of a 21-residue A chain and a 31-residue B chain. The sequence of the A chain is GIVEQCCHKPCTIYELENYCN. The sequence of the B chain is AANQHLCGSHLVEALYLVCGEKGFFYNPNKV. |
| doi_str_mv | 10.1016/0016-6480(87)90192-4 |
| format | Article |
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Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactivities during purification of the two peptides. The sequence of the 36-amino acid peptide containing a C-terminal tyrosinamide was identical at 31 of 36 positions to porcine neuropeptide Y (NPY). The amino acid sequence of this peptide is YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH
2. The second peptide, gar insulin, contains 52 amino acid residues and is composed of a 21-residue A chain and a 31-residue B chain. The sequence of the A chain is GIVEQCCHKPCTIYELENYCN. The sequence of the B chain is AANQHLCGSHLVEALYLVCGEKGFFYNPNKV.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/0016-6480(87)90192-4</identifier><identifier>PMID: 3311873</identifier><identifier>CODEN: GCENA5</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; amino acids ; Animals ; Biological and medical sciences ; Endocrine pancreas ; Fishes - physiology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Hormones. Régulation ; insulin ; Insulin - isolation & purification ; Islets of Langerhans - analysis ; Lepisosteus spatula ; Macromolecular Substances ; Molecular Sequence Data ; molecular structure ; pancreatic peptide ; Pancreatic Polypeptide - isolation & purification ; Peptide Fragments - analysis ; polypeptides ; Sequence Homology, Nucleic Acid ; Species Specificity ; Vertebrates: endocrinology</subject><ispartof>General and comparative endocrinology, 1987-09, Vol.67 (3), p.375-382</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-51c01453d6fb065f426bc13e866e640960ec8502e77074578845e345ac2755b83</citedby><cites>FETCH-LOGICAL-c417t-51c01453d6fb065f426bc13e866e640960ec8502e77074578845e345ac2755b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0016648087901924$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7629194$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3311873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollock, H.G.</creatorcontrib><creatorcontrib>Kimmel, J.R.</creatorcontrib><creatorcontrib>Hamilton, J.W.</creatorcontrib><creatorcontrib>Rouse, J.B.</creatorcontrib><creatorcontrib>Ebner, K.E.</creatorcontrib><creatorcontrib>Lance, V.</creatorcontrib><creatorcontrib>Rawitch, A.B.</creatorcontrib><title>Isolation and structures of alligator gar ( Lepisosteus spatula) insulin and pancreatic polypeptide</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>Insulin and a 36-residue peptide with homology to pancreatic polypeptide (PP) were isolated from the endocrine pancreas of the alligator gar (
Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactivities during purification of the two peptides. The sequence of the 36-amino acid peptide containing a C-terminal tyrosinamide was identical at 31 of 36 positions to porcine neuropeptide Y (NPY). The amino acid sequence of this peptide is YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH
2. The second peptide, gar insulin, contains 52 amino acid residues and is composed of a 21-residue A chain and a 31-residue B chain. The sequence of the A chain is GIVEQCCHKPCTIYELENYCN. The sequence of the B chain is AANQHLCGSHLVEALYLVCGEKGFFYNPNKV.</description><subject>Amino Acid Sequence</subject><subject>amino acids</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Endocrine pancreas</subject><subject>Fishes - physiology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones. Régulation</subject><subject>insulin</subject><subject>Insulin - isolation & purification</subject><subject>Islets of Langerhans - analysis</subject><subject>Lepisosteus spatula</subject><subject>Macromolecular Substances</subject><subject>Molecular Sequence Data</subject><subject>molecular structure</subject><subject>pancreatic peptide</subject><subject>Pancreatic Polypeptide - isolation & purification</subject><subject>Peptide Fragments - analysis</subject><subject>polypeptides</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>Vertebrates: endocrinology</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpSDdJ_0ELOpSSHNxI1qcvgRKaNrDQS3sWWnkcVLSWq5EL-fexu8se28vMYZ55GZ4h5B1nnzjj-pYtpdHSsmtrbjrGu7aRr8iGs0412kr2mmxOyBtygfiLMaaE5ufkXAjOrREbEh4xJ19jHqkfe4q1zKHOBZDmgfqU4pOvudAnX-g13cIUMWOFGSlOvs7J39A44pziYX3yYyiwxAU65fQ8wVRjD1fkbPAJ4e2xX5KfD19-3H9rtt-_Pt5_3jZBclMbxQPjUoleDzum1SBbvQtcgNUatGSdZhCsYi0Yw4xUxlqpQEjlQ2uU2llxST4ecqeSf8-A1e0jBkjJj5BndJYztXiR_wX5Ysxo0S6gPIChZMQCg5tK3Pvy7Dhz6xPcatithp017u8T3Jr__pg_7_bQn5aO1pf5h-PcY_BpKIu2iCfM6Lbj3Rpzd8BgkfYnQnEYIowB-lggVNfn-O87XgCl9qH5</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Pollock, H.G.</creator><creator>Kimmel, J.R.</creator><creator>Hamilton, J.W.</creator><creator>Rouse, J.B.</creator><creator>Ebner, K.E.</creator><creator>Lance, V.</creator><creator>Rawitch, A.B.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19870901</creationdate><title>Isolation and structures of alligator gar ( Lepisosteus spatula) insulin and pancreatic polypeptide</title><author>Pollock, H.G. ; Kimmel, J.R. ; Hamilton, J.W. ; Rouse, J.B. ; Ebner, K.E. ; Lance, V. ; Rawitch, A.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-51c01453d6fb065f426bc13e866e640960ec8502e77074578845e345ac2755b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acid Sequence</topic><topic>amino acids</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Endocrine pancreas</topic><topic>Fishes - physiology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones. Régulation</topic><topic>insulin</topic><topic>Insulin - isolation & purification</topic><topic>Islets of Langerhans - analysis</topic><topic>Lepisosteus spatula</topic><topic>Macromolecular Substances</topic><topic>Molecular Sequence Data</topic><topic>molecular structure</topic><topic>pancreatic peptide</topic><topic>Pancreatic Polypeptide - isolation & purification</topic><topic>Peptide Fragments - analysis</topic><topic>polypeptides</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pollock, H.G.</creatorcontrib><creatorcontrib>Kimmel, J.R.</creatorcontrib><creatorcontrib>Hamilton, J.W.</creatorcontrib><creatorcontrib>Rouse, J.B.</creatorcontrib><creatorcontrib>Ebner, K.E.</creatorcontrib><creatorcontrib>Lance, V.</creatorcontrib><creatorcontrib>Rawitch, A.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pollock, H.G.</au><au>Kimmel, J.R.</au><au>Hamilton, J.W.</au><au>Rouse, J.B.</au><au>Ebner, K.E.</au><au>Lance, V.</au><au>Rawitch, A.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and structures of alligator gar ( Lepisosteus spatula) insulin and pancreatic polypeptide</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>67</volume><issue>3</issue><spage>375</spage><epage>382</epage><pages>375-382</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><coden>GCENA5</coden><abstract>Insulin and a 36-residue peptide with homology to pancreatic polypeptide (PP) were isolated from the endocrine pancreas of the alligator gar (
Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactivities during purification of the two peptides. The sequence of the 36-amino acid peptide containing a C-terminal tyrosinamide was identical at 31 of 36 positions to porcine neuropeptide Y (NPY). The amino acid sequence of this peptide is YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH
2. The second peptide, gar insulin, contains 52 amino acid residues and is composed of a 21-residue A chain and a 31-residue B chain. The sequence of the A chain is GIVEQCCHKPCTIYELENYCN. The sequence of the B chain is AANQHLCGSHLVEALYLVCGEKGFFYNPNKV.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3311873</pmid><doi>10.1016/0016-6480(87)90192-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | General and comparative endocrinology, 1987-09, Vol.67 (3), p.375-382 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence amino acids Animals Biological and medical sciences Endocrine pancreas Fishes - physiology Freshwater Fundamental and applied biological sciences. Psychology Hormones. Régulation insulin Insulin - isolation & purification Islets of Langerhans - analysis Lepisosteus spatula Macromolecular Substances Molecular Sequence Data molecular structure pancreatic peptide Pancreatic Polypeptide - isolation & purification Peptide Fragments - analysis polypeptides Sequence Homology, Nucleic Acid Species Specificity Vertebrates: endocrinology |
| title | Isolation and structures of alligator gar ( Lepisosteus spatula) insulin and pancreatic polypeptide |
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