A Glycoprotein Inhibitor of In Vitro Granulopoiesis Associated With AIDS
Patients infected with the human immunodeficiency virus (HIV) often present with neutropenia. To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within t...
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| Veröffentlicht in: | Blood 1987-11, Vol.70 (5), p.1267-1272 |
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| creator | Leiderman, Ira Z. Greenberg, Michael L. Adelsberg, Bernard R. Siegal, Frederick P. |
| description | Patients infected with the human immunodeficiency virus (HIV) often present with neutropenia. To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within the AIDS spectrum manifesting symptoms or signs related to HIV infection. Of these, 70 had a significant deficit in the growth of this committed progenitor when compared with normal controls (P .4). Analysis of these conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed that this gp possessed the inhibitory activity. These data suggest that this gp may be an important factor in HIV-related neutropenia. The presence of gp84 was independent of drugs administered to the patients. © 1987 by Grune & Stratton, Inc. |
| doi_str_mv | 10.1182/blood.V70.5.1267.1267 |
| format | Article |
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To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within the AIDS spectrum manifesting symptoms or signs related to HIV infection. Of these, 70 had a significant deficit in the growth of this committed progenitor when compared with normal controls (P <.01). Further analysis revealed that the nucleated bone marrow cells from AIDS and AIDS-related complex (ARC) patients inhibited the growth of CFU-GMs from normal individuals when cocultured in agar (P < .001). Control CFU-GMs were also inhibited when they were cultured over feeder layers containing patients’ BM cells (P < .001). Conditioned media obtained from the liquid culture of patients’ BM cells did not inhibit normal control CFU-GM growth to a degree different from that of the cells themselves (P > .4). Analysis of these conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed that this gp possessed the inhibitory activity. These data suggest that this gp may be an important factor in HIV-related neutropenia. The presence of gp84 was independent of drugs administered to the patients. © 1987 by Grune & Stratton, Inc.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V70.5.1267.1267</identifier><identifier>PMID: 3663934</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Acquired Immunodeficiency Syndrome - physiopathology ; AIDS/HIV ; Biological and medical sciences ; Bone Marrow - pathology ; Cells, Cultured ; Colony-Forming Units Assay ; Female ; Glycoproteins - isolation & purification ; Glycoproteins - physiology ; Granulocytes - cytology ; Hematopoiesis ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Leukopenia - etiology ; Male ; Medical sciences</subject><ispartof>Blood, 1987-11, Vol.70 (5), p.1267-1272</ispartof><rights>1987 American Society of Hematology</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2837-3bab63d5dd8293110c04e97ad6fbfd8f67bdd1965ad729c664353902dd76db073</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7415985$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3663934$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leiderman, Ira Z.</creatorcontrib><creatorcontrib>Greenberg, Michael L.</creatorcontrib><creatorcontrib>Adelsberg, Bernard R.</creatorcontrib><creatorcontrib>Siegal, Frederick P.</creatorcontrib><title>A Glycoprotein Inhibitor of In Vitro Granulopoiesis Associated With AIDS</title><title>Blood</title><addtitle>Blood</addtitle><description>Patients infected with the human immunodeficiency virus (HIV) often present with neutropenia. To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within the AIDS spectrum manifesting symptoms or signs related to HIV infection. Of these, 70 had a significant deficit in the growth of this committed progenitor when compared with normal controls (P <.01). Further analysis revealed that the nucleated bone marrow cells from AIDS and AIDS-related complex (ARC) patients inhibited the growth of CFU-GMs from normal individuals when cocultured in agar (P < .001). Control CFU-GMs were also inhibited when they were cultured over feeder layers containing patients’ BM cells (P < .001). Conditioned media obtained from the liquid culture of patients’ BM cells did not inhibit normal control CFU-GM growth to a degree different from that of the cells themselves (P > .4). Analysis of these conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed that this gp possessed the inhibitory activity. These data suggest that this gp may be an important factor in HIV-related neutropenia. The presence of gp84 was independent of drugs administered to the patients. © 1987 by Grune & Stratton, Inc.</description><subject>Acquired Immunodeficiency Syndrome - physiopathology</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - pathology</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Female</subject><subject>Glycoproteins - isolation & purification</subject><subject>Glycoproteins - physiology</subject><subject>Granulocytes - cytology</subject><subject>Hematopoiesis</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Leukopenia - etiology</subject><subject>Male</subject><subject>Medical sciences</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LwzAUwIMoc07_hEEP4q0zH03SnqRM3QYDD-o8hjRJWaRrZtIJ--_NtrKrl_d4vN_74AfAGMEJQjl-rBrn9GTF4YROEGb8GC7AEFGcpxBieAmGEEKWZgVH1-AmhG8IUUYwHYABYYwUJBuCeZnMmr1yW-86Y9tk0a5tZTvnE1fHIlnZzrtk5mW7a9zWWRNsSMoQnLKyMzr5st06KRfP77fgqpZNMHd9HoHP15eP6Txdvs0W03KZKpwTnpJKVoxoqnWOC4IQVDAzBZea1VWt85rxSmtUMCo1x4ViLCOUFBBrzZmuICcj8HDaGz_-2ZnQiY0NyjSNbI3bBZEjSDOCWATpCVTeheBNLbbebqTfCwTFwaA4GhTRoKDiIO8Y4ty4P7CrNkafp3plsX_f92VQsqmjGmXDGeMZokVOI_Z0wkyU8WuNF0FZ0yqjrTeqE9rZfx75AytGjvs</recordid><startdate>198711</startdate><enddate>198711</enddate><creator>Leiderman, Ira Z.</creator><creator>Greenberg, Michael L.</creator><creator>Adelsberg, Bernard R.</creator><creator>Siegal, Frederick P.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198711</creationdate><title>A Glycoprotein Inhibitor of In Vitro Granulopoiesis Associated With AIDS</title><author>Leiderman, Ira Z. ; Greenberg, Michael L. ; Adelsberg, Bernard R. ; Siegal, Frederick P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2837-3bab63d5dd8293110c04e97ad6fbfd8f67bdd1965ad729c664353902dd76db073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Acquired Immunodeficiency Syndrome - physiopathology</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - pathology</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Female</topic><topic>Glycoproteins - isolation & purification</topic><topic>Glycoproteins - physiology</topic><topic>Granulocytes - cytology</topic><topic>Hematopoiesis</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Leukopenia - etiology</topic><topic>Male</topic><topic>Medical sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leiderman, Ira Z.</creatorcontrib><creatorcontrib>Greenberg, Michael L.</creatorcontrib><creatorcontrib>Adelsberg, Bernard R.</creatorcontrib><creatorcontrib>Siegal, Frederick P.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leiderman, Ira Z.</au><au>Greenberg, Michael L.</au><au>Adelsberg, Bernard R.</au><au>Siegal, Frederick P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Glycoprotein Inhibitor of In Vitro Granulopoiesis Associated With AIDS</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1987-11</date><risdate>1987</risdate><volume>70</volume><issue>5</issue><spage>1267</spage><epage>1272</epage><pages>1267-1272</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Patients infected with the human immunodeficiency virus (HIV) often present with neutropenia. To elucidate the mechanism(s) of this HIV-related neutropenia, we assessed the proliferative capacity of the granulocyte-macrophage progenitor cell (CFU-GM) from the bone marrow (BM) of 78 patients within the AIDS spectrum manifesting symptoms or signs related to HIV infection. Of these, 70 had a significant deficit in the growth of this committed progenitor when compared with normal controls (P <.01). Further analysis revealed that the nucleated bone marrow cells from AIDS and AIDS-related complex (ARC) patients inhibited the growth of CFU-GMs from normal individuals when cocultured in agar (P < .001). Control CFU-GMs were also inhibited when they were cultured over feeder layers containing patients’ BM cells (P < .001). Conditioned media obtained from the liquid culture of patients’ BM cells did not inhibit normal control CFU-GM growth to a degree different from that of the cells themselves (P > .4). Analysis of these conditioned media by polyacrylamide gel electrophoresis (PAGE) revealed a unique glycoprotein (gp) with a mol wt of 84 kd. Further studies revealed that this gp possessed the inhibitory activity. These data suggest that this gp may be an important factor in HIV-related neutropenia. The presence of gp84 was independent of drugs administered to the patients. © 1987 by Grune & Stratton, Inc.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>3663934</pmid><doi>10.1182/blood.V70.5.1267.1267</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1987-11, Vol.70 (5), p.1267-1272 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acquired Immunodeficiency Syndrome - physiopathology AIDS/HIV Biological and medical sciences Bone Marrow - pathology Cells, Cultured Colony-Forming Units Assay Female Glycoproteins - isolation & purification Glycoproteins - physiology Granulocytes - cytology Hematopoiesis Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Leukopenia - etiology Male Medical sciences |
| title | A Glycoprotein Inhibitor of In Vitro Granulopoiesis Associated With AIDS |
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