Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene

A human umbilical vein endothelial cell cDNA library in lambda gt11 was screened for expression of thrombomodulin antigens with affinity-purified rabbit polyclonal anti-thrombomodulin immunoglobulin G (IgG) and mouse monoclonal anti-human thrombomodulin IgG. Among 7 million recombinant clones screen...

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Veröffentlicht in:	Biochemistry (Easton) 1987-07, Vol.26 (14), p.4350-4357
Hauptverfasser:	Wen, Duanzhi, Dittman, William A, Ye, Richard D, Deaven, Larry L, Majerus, Philip W, Sadler, J. Evan
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques 550401 - Genetics- Tracer Techniques AMINO ACID SEQUENCE ANIMAL TISSUES ANIMALS ANTIBODIES Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BLOOD VESSELS BODY CARBOHYDRATES CARDIOVASCULAR SYSTEM cDNA Chromosome Mapping Chromosomes, Human, Pair 20 Cloning, Molecular COAGULANTS CONFIGURATION INTERACTION DAYS LIVING RADIOISOTOPES DNA DNA - analysis DNA SEQUENCING DRUGS ENDOTHELIUM Endothelium - metabolism ENZYMES EVEN-ODD NUCLEI Female Fundamental and applied biological sciences. Psychology Genes Genes. Genome GENETIC MAPPING GLUCOPROTEINS HEMATOLOGIC AGENTS HEMOSTATICS Humans HYDROLASES ISOTOPES LABELLED COMPOUNDS LIGHT NUCLEI MAMMALS MAN MAPPING MEMBRANE PROTEINS Molecular and cellular biology Molecular genetics MOLECULAR STRUCTURE MONOCLONAL ANTIBODIES NUCLEI NUCLEIC ACIDS nucleotide sequence ODD-ODD NUCLEI ORGANIC COMPOUNDS ORGANS Peptide Fragments - analysis PEPTIDE HYDROLASES PHOSPHORUS 32 PHOSPHORUS ISOTOPES PRIMATES PROTEINS RADIOISOTOPES RECEPTORS Receptors, Cell Surface - genetics Receptors, Thrombin RECOMBINANT DNA SACCHARIDES Sequence Homology, Nucleic Acid SERINE PROTEINASES STRUCTURAL CHEMICAL ANALYSIS SULFUR 35 SULFUR ISOTOPES THROMBIN thrombomodulin Tissue Distribution TISSUES Trypsin Umbilical Veins - metabolism VEINS VERTEBRATES
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