Reactivation of cytoplasmic actomyosin in Physarum plasmodia extracted with glycerol and dimethylsulphoxide

Thin-spread plasmodia of Physarum were subjected to extraction procedures using 50% glycerol or DMSO (dimethylsulphoxide) followed by labelling of actin with fluorescent phallotoxins. During the reactivation of the actomyosin system by 2 mM-MgATP fluorescent actin fibres contract isotonically, which...

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Veröffentlicht in:	Journal of cell science 1987-03, Vol.87 (2), p.231-239
Hauptverfasser:	BELL, R, ACHENBACH, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Actomyosin - metabolism Biological and medical sciences Calcium - physiology Cell structures and functions Cytoplasm - metabolism Cytoskeleton, cytoplasm. Intracellular movements Dimethyl Sulfoxide Fundamental and applied biological sciences. Psychology Glycerol Microscopy, Fluorescence Molecular and cellular biology Physarum - metabolism
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