Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl
GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromat...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1987-10, Vol.262 (29), p.14255-14259 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14259 |
|---|---|
| container_issue | 29 |
| container_start_page | 14255 |
| container_title | The Journal of biological chemistry |
| container_volume | 262 |
| creator | Bedford, J J Bagnasco, S M Kador, P F Harris, H W Burg, M B |
| description | GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (alditol:NADPH+ oxidoreductase, EC 1.1.1.21). Goat antiserum against this purified aldose reductase selected the 39-kDa band from immunoblots of cells grown in a medium containing high NaCl. When the osmolality of the medium was increased by adding NaCl, the amount of aldose reductase protein and the aldose reductase activity increased together from very low to sustained high levels over several days. The aldose reductase protein was more than 10% of the soluble cell protein when cells were propagated in medium made hyperosmotic by adding NaCl to increase medium osmolality to 600 mosm.kg-1. |
| doi_str_mv | 10.1016/S0021-9258(18)47931-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81026095</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021925818479318</els_id><sourcerecordid>81026095</sourcerecordid><originalsourceid>FETCH-LOGICAL-c562t-4dff6406e95ef10ed3c42b2dc47d67f4e1fae75288793b120ededd00b7d6231d3</originalsourceid><addsrcrecordid>eNqFUU1v1DAQjRCoLIWfUMkHhEBqwOPEWedUoVX5kCo4ABI3y7EnXSMnXuwEWn4Iv5dJd7Uc68vYM2_Gb94rijPgr4FD8-YL5wLKVkj1EtSret1WUKoHxQq4qspKwveHxeoIeVw8yfkHp1O3cFKcVACyVe2q-LvZmmTshMn_MZOPIzOjY7s5-d7bfSL2zLDBDIMJ3tAzDzHh9RzMFNMt26U4oR_PmQkuZmQJ3Wwnk_Gc-ZGu6ChSdjSBDVQLwVCXxRAy627Z1l9vGd5MxIFSNDSxT2YTnhaPehMyPjvE0-Lbu8uvmw_l1ef3Hzdvr0orGzGVtev7puYNthJ74OgqW4tOOFuvXbPua4Te4FoKpUieDgQh0DnOOyqLClx1WrzYz6U1fs6YJz34vDAxI8Y5awVcNLyV9wKhbmspm5aAcg-0KeacsNe75AfaWQPXi3H6zji9uKJB6TvjtKK-s8MHc0c6HbsOTlH9-aFusjWhT2a0Ph9hawk1qOY_bBH2t0-oOx_tFgctGqFFS0SFXNa52MOQxP3lMelsPY7kFrXYSbvo7-H7D2wDxBk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14945569</pqid></control><display><type>article</type><title>Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Bedford, J J ; Bagnasco, S M ; Kador, P F ; Harris, H W ; Burg, M B</creator><creatorcontrib>Bedford, J J ; Bagnasco, S M ; Kador, P F ; Harris, H W ; Burg, M B</creatorcontrib><description>GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (alditol:NADPH+ oxidoreductase, EC 1.1.1.21). Goat antiserum against this purified aldose reductase selected the 39-kDa band from immunoblots of cells grown in a medium containing high NaCl. When the osmolality of the medium was increased by adding NaCl, the amount of aldose reductase protein and the aldose reductase activity increased together from very low to sustained high levels over several days. The aldose reductase protein was more than 10% of the soluble cell protein when cells were propagated in medium made hyperosmotic by adding NaCl to increase medium osmolality to 600 mosm.kg-1.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)47931-8</identifier><identifier>PMID: 3115989</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Aldehyde Reductase - biosynthesis ; Aldehyde Reductase - isolation & purification ; Aldehyde Reductase - metabolism ; aldose dehydrogenase ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cell Line ; Enzyme Induction ; Enzymes and enzyme inhibitors ; Epithelium - enzymology ; Fundamental and applied biological sciences. Psychology ; Immunodiffusion ; Kidney Medulla - drug effects ; Kidney Medulla - enzymology ; Kinetics ; medulla ; Molecular Weight ; osmoregulation ; Oxidoreductases ; Pancreatitis-Associated Proteins ; Rabbits ; Sodium Chloride - pharmacology ; Substrate Specificity ; Sugar Alcohol Dehydrogenases - metabolism</subject><ispartof>The Journal of biological chemistry, 1987-10, Vol.262 (29), p.14255-14259</ispartof><rights>1987 © 1987 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-4dff6406e95ef10ed3c42b2dc47d67f4e1fae75288793b120ededd00b7d6231d3</citedby><cites>FETCH-LOGICAL-c562t-4dff6406e95ef10ed3c42b2dc47d67f4e1fae75288793b120ededd00b7d6231d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7514186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3115989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bedford, J J</creatorcontrib><creatorcontrib>Bagnasco, S M</creatorcontrib><creatorcontrib>Kador, P F</creatorcontrib><creatorcontrib>Harris, H W</creatorcontrib><creatorcontrib>Burg, M B</creatorcontrib><title>Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (alditol:NADPH+ oxidoreductase, EC 1.1.1.21). Goat antiserum against this purified aldose reductase selected the 39-kDa band from immunoblots of cells grown in a medium containing high NaCl. When the osmolality of the medium was increased by adding NaCl, the amount of aldose reductase protein and the aldose reductase activity increased together from very low to sustained high levels over several days. The aldose reductase protein was more than 10% of the soluble cell protein when cells were propagated in medium made hyperosmotic by adding NaCl to increase medium osmolality to 600 mosm.kg-1.</description><subject>Aldehyde Reductase - biosynthesis</subject><subject>Aldehyde Reductase - isolation & purification</subject><subject>Aldehyde Reductase - metabolism</subject><subject>aldose dehydrogenase</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Enzyme Induction</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Epithelium - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunodiffusion</subject><subject>Kidney Medulla - drug effects</subject><subject>Kidney Medulla - enzymology</subject><subject>Kinetics</subject><subject>medulla</subject><subject>Molecular Weight</subject><subject>osmoregulation</subject><subject>Oxidoreductases</subject><subject>Pancreatitis-Associated Proteins</subject><subject>Rabbits</subject><subject>Sodium Chloride - pharmacology</subject><subject>Substrate Specificity</subject><subject>Sugar Alcohol Dehydrogenases - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQjRCoLIWfUMkHhEBqwOPEWedUoVX5kCo4ABI3y7EnXSMnXuwEWn4Iv5dJd7Uc68vYM2_Gb94rijPgr4FD8-YL5wLKVkj1EtSret1WUKoHxQq4qspKwveHxeoIeVw8yfkHp1O3cFKcVACyVe2q-LvZmmTshMn_MZOPIzOjY7s5-d7bfSL2zLDBDIMJ3tAzDzHh9RzMFNMt26U4oR_PmQkuZmQJ3Wwnk_Gc-ZGu6ChSdjSBDVQLwVCXxRAy627Z1l9vGd5MxIFSNDSxT2YTnhaPehMyPjvE0-Lbu8uvmw_l1ef3Hzdvr0orGzGVtev7puYNthJ74OgqW4tOOFuvXbPua4Te4FoKpUieDgQh0DnOOyqLClx1WrzYz6U1fs6YJz34vDAxI8Y5awVcNLyV9wKhbmspm5aAcg-0KeacsNe75AfaWQPXi3H6zji9uKJB6TvjtKK-s8MHc0c6HbsOTlH9-aFusjWhT2a0Ph9hawk1qOY_bBH2t0-oOx_tFgctGqFFS0SFXNa52MOQxP3lMelsPY7kFrXYSbvo7-H7D2wDxBk</recordid><startdate>19871015</startdate><enddate>19871015</enddate><creator>Bedford, J J</creator><creator>Bagnasco, S M</creator><creator>Kador, P F</creator><creator>Harris, H W</creator><creator>Burg, M B</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19871015</creationdate><title>Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl</title><author>Bedford, J J ; Bagnasco, S M ; Kador, P F ; Harris, H W ; Burg, M B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-4dff6406e95ef10ed3c42b2dc47d67f4e1fae75288793b120ededd00b7d6231d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Aldehyde Reductase - biosynthesis</topic><topic>Aldehyde Reductase - isolation & purification</topic><topic>Aldehyde Reductase - metabolism</topic><topic>aldose dehydrogenase</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Enzyme Induction</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Epithelium - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunodiffusion</topic><topic>Kidney Medulla - drug effects</topic><topic>Kidney Medulla - enzymology</topic><topic>Kinetics</topic><topic>medulla</topic><topic>Molecular Weight</topic><topic>osmoregulation</topic><topic>Oxidoreductases</topic><topic>Pancreatitis-Associated Proteins</topic><topic>Rabbits</topic><topic>Sodium Chloride - pharmacology</topic><topic>Substrate Specificity</topic><topic>Sugar Alcohol Dehydrogenases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bedford, J J</creatorcontrib><creatorcontrib>Bagnasco, S M</creatorcontrib><creatorcontrib>Kador, P F</creatorcontrib><creatorcontrib>Harris, H W</creatorcontrib><creatorcontrib>Burg, M B</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bedford, J J</au><au>Bagnasco, S M</au><au>Kador, P F</au><au>Harris, H W</au><au>Burg, M B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1987-10-15</date><risdate>1987</risdate><volume>262</volume><issue>29</issue><spage>14255</spage><epage>14259</epage><pages>14255-14259</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (alditol:NADPH+ oxidoreductase, EC 1.1.1.21). Goat antiserum against this purified aldose reductase selected the 39-kDa band from immunoblots of cells grown in a medium containing high NaCl. When the osmolality of the medium was increased by adding NaCl, the amount of aldose reductase protein and the aldose reductase activity increased together from very low to sustained high levels over several days. The aldose reductase protein was more than 10% of the soluble cell protein when cells were propagated in medium made hyperosmotic by adding NaCl to increase medium osmolality to 600 mosm.kg-1.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3115989</pmid><doi>10.1016/S0021-9258(18)47931-8</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1987-10, Vol.262 (29), p.14255-14259 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81026095 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aldehyde Reductase - biosynthesis Aldehyde Reductase - isolation & purification Aldehyde Reductase - metabolism aldose dehydrogenase Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Enzyme Induction Enzymes and enzyme inhibitors Epithelium - enzymology Fundamental and applied biological sciences. Psychology Immunodiffusion Kidney Medulla - drug effects Kidney Medulla - enzymology Kinetics medulla Molecular Weight osmoregulation Oxidoreductases Pancreatitis-Associated Proteins Rabbits Sodium Chloride - pharmacology Substrate Specificity Sugar Alcohol Dehydrogenases - metabolism |
| title | Characterization and purification of a mammalian osmoregulatory protein, aldose reductase, induced in renal medullary cells by high extracellular NaCl |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T11%3A45%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20and%20purification%20of%20a%20mammalian%20osmoregulatory%20protein,%20aldose%20reductase,%20induced%20in%20renal%20medullary%20cells%20by%20high%20extracellular%20NaCl&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Bedford,%20J%20J&rft.date=1987-10-15&rft.volume=262&rft.issue=29&rft.spage=14255&rft.epage=14259&rft.pages=14255-14259&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(18)47931-8&rft_dat=%3Cproquest_cross%3E81026095%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14945569&rft_id=info:pmid/3115989&rft_els_id=S0021925818479318&rfr_iscdi=true |