Cloning of the vaccinia virus telomere in a yeast plasmid vector
The vaccinia virus DNA telomere, which contains a covalently closed hairpin structure, has been cloned in a yeast plasmid vector. Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within...
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| Veröffentlicht in: | Gene 1984, Vol.27 (1), p.13-21 |
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| container_title | Gene |
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| creator | DeLange, Aloysius M. Futcher, Bruce Morgan, Richard McFadden, Grant |
| description | The vaccinia virus DNA telomere, which contains a covalently closed hairpin structure, has been cloned in a yeast plasmid vector. Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within a circular plasmid, with the sequences of the viral hairpin now at the axis of symmetry of an imperfect palindrome. As such, the cloned telomere resembles the telomeric replicative intermediate observed during vaccinia virus DNA replication. Small deletions and duplications in the viral inverted repeats of different clones suggest a model in which the observed circular plasmids were generated in yeast by the replication of hybrid linear DNA molecules consisting of the linearized yeast vector flanked by two hairpin-containing vaccinia termini. |
| doi_str_mv | 10.1016/0378-1119(84)90234-8 |
| format | Article |
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Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within a circular plasmid, with the sequences of the viral hairpin now at the axis of symmetry of an imperfect palindrome. As such, the cloned telomere resembles the telomeric replicative intermediate observed during vaccinia virus DNA replication. Small deletions and duplications in the viral inverted repeats of different clones suggest a model in which the observed circular plasmids were generated in yeast by the replication of hybrid linear DNA molecules consisting of the linearized yeast vector flanked by two hairpin-containing vaccinia termini.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(84)90234-8</identifier><identifier>PMID: 6325299</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA Replication ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Hairpin termini ; Methods. Procedures. 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Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within a circular plasmid, with the sequences of the viral hairpin now at the axis of symmetry of an imperfect palindrome. As such, the cloned telomere resembles the telomeric replicative intermediate observed during vaccinia virus DNA replication. Small deletions and duplications in the viral inverted repeats of different clones suggest a model in which the observed circular plasmids were generated in yeast by the replication of hybrid linear DNA molecules consisting of the linearized yeast vector flanked by two hairpin-containing vaccinia termini.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA Replication</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Hairpin termini</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Molecular cloning</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Conformation</subject><subject>palindrome</subject><subject>Plasmids</subject><subject>recombinant DNA</subject><subject>Replication</subject><subject>replicative intermediate</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>vaccinia virus</subject><subject>Vaccinia virus - genetics</subject><subject>Virus Replication</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotX78A4UcRPSwmkmy2eQiSvELCl70HGJ2ViP7UZNtof_erS096lzm8D4zvDyEnAC7AgbqmolCZwBgLrS8NIwLmekdMgZdmIwxoXfJeIvsk4OUvtgwec5HZKQEz7kxY3I7qbs2tB-0q2j_iXThvA9tcHQR4jzRHuuuwYg0tNTRJbrU01ntUhNKukDfd_GI7FWuTni82Yfk7eH-dfKUTV8enyd308xLKPoMDJelUsKXqLzWQw3JlZIl84o7XTmTCycM84xXBSiei1Iid0ZWrEJdFEwckvP131nsvueYetuE5LGuXYvdPFkNjEsu4V8QhOaQCz2Acg362KUUsbKzGBoXlxaYXRm2K312pc9qaX8N29XZ6eb__L3Bcnu0UTrkZ5vcJe_qKrrWh7TFjFJQgBiwmzWGg7RFwGiTD9h6LEMcxNqyC3_3-AHMepTF</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>DeLange, Aloysius M.</creator><creator>Futcher, Bruce</creator><creator>Morgan, Richard</creator><creator>McFadden, Grant</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1984</creationdate><title>Cloning of the vaccinia virus telomere in a yeast plasmid vector</title><author>DeLange, Aloysius M. ; Futcher, Bruce ; Morgan, Richard ; McFadden, Grant</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-1924d663cde6c8805542664d0c62a8fa953a390c02f716253d4e2a94f0fe87703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA Replication</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Hairpin termini</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Molecular cloning</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Conformation</topic><topic>palindrome</topic><topic>Plasmids</topic><topic>recombinant DNA</topic><topic>Replication</topic><topic>replicative intermediate</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>vaccinia virus</topic><topic>Vaccinia virus - genetics</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeLange, Aloysius M.</creatorcontrib><creatorcontrib>Futcher, Bruce</creatorcontrib><creatorcontrib>Morgan, Richard</creatorcontrib><creatorcontrib>McFadden, Grant</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeLange, Aloysius M.</au><au>Futcher, Bruce</au><au>Morgan, Richard</au><au>McFadden, Grant</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the vaccinia virus telomere in a yeast plasmid vector</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1984</date><risdate>1984</risdate><volume>27</volume><issue>1</issue><spage>13</spage><epage>21</epage><pages>13-21</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The vaccinia virus DNA telomere, which contains a covalently closed hairpin structure, has been cloned in a yeast plasmid vector. Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within a circular plasmid, with the sequences of the viral hairpin now at the axis of symmetry of an imperfect palindrome. As such, the cloned telomere resembles the telomeric replicative intermediate observed during vaccinia virus DNA replication. Small deletions and duplications in the viral inverted repeats of different clones suggest a model in which the observed circular plasmids were generated in yeast by the replication of hybrid linear DNA molecules consisting of the linearized yeast vector flanked by two hairpin-containing vaccinia termini.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6325299</pmid><doi>10.1016/0378-1119(84)90234-8</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1984, Vol.27 (1), p.13-21 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biotechnology Cloning, Molecular DNA Replication DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Genes, Viral Genetic engineering Genetic technics Genetic Vectors Hairpin termini Methods. Procedures. Technologies Molecular and cellular biology Molecular cloning Molecular genetics Nucleic Acid Conformation palindrome Plasmids recombinant DNA Replication replicative intermediate Saccharomyces cerevisiae - genetics vaccinia virus Vaccinia virus - genetics Virus Replication |
| title | Cloning of the vaccinia virus telomere in a yeast plasmid vector |
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