Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3
A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyv...
Gespeichert in:
| Veröffentlicht in: | Archives of biochemistry and biophysics 1984-04, Vol.230 (1), p.21-29 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 29 |
|---|---|
| container_issue | 1 |
| container_start_page | 21 |
| container_title | Archives of biochemistry and biophysics |
| container_volume | 230 |
| creator | Simpson, R.U Wichmann, J.K Paaren, H.E Schnoes, H.K Deluca, H.F |
| description | A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites. |
| doi_str_mv | 10.1016/0003-9861(84)90082-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81024191</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>81024191</sourcerecordid><originalsourceid>FETCH-LOGICAL-c241t-1b37e2070641d65f330647c3e5a31d2ac4c61febb5cec2761c753bd59295cee93</originalsourceid><addsrcrecordid>eNptUcFO3TAQtBAVfVD-oKg-ITiY7tqJk3BDj5YiUfUAnC3HcYrbJAbbQeQ_-sFNeE-cOO1qZ3ZGO0vIZ4QzBJRfAUCwqpR4UmanFUDJGe6QFUIlGYgy2yWrN8pHsh_jHwDETPI9sicL5FzwFfn30yZd-87FnvqW8pw9TE3wL9OzS7p3A70UtJ5o0In-dc1gJ2ps10U6I2bs0hjsOXXRdzo5P1A9NNQ1dkiudWYzmkWNi-wVSkEPkWHFBh8YAvMvnr3n-Il8aHUX7eG2HpD779_u1j_Yza-r6_XFDTM8w8SwFoXlUIDMsJF5K8TcFUbYXAtsuDaZkdjaus6NNbyQaIpc1E1e8Wqe2EockOON7mPwT6ONSfUuLvfpwfoxqhJhNqpwJmYbogk-xmBb9Rhcr8OkENTyDLUkrZakVZmp12eoZe1oqz_WvW3elrbpz_iXDd5qr_Tv4KK6v-WAAnguSglS_Af9yo4j</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81024191</pqid></control><display><type>article</type><title>Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Simpson, R.U ; Wichmann, J.K ; Paaren, H.E ; Schnoes, H.K ; Deluca, H.F</creator><creatorcontrib>Simpson, R.U ; Wichmann, J.K ; Paaren, H.E ; Schnoes, H.K ; Deluca, H.F</creatorcontrib><description>A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(84)90082-1</identifier><identifier>PMID: 6712232</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Biotransformation ; Calcifediol - analogs & derivatives ; Calcifediol - isolation & purification ; Calcifediol - metabolism ; Cells, Cultured ; Chickens ; Chromatography, High Pressure Liquid ; human nutrition ; Kidney - metabolism ; Male ; nutrition physiology ; Rats ; Spectrum Analysis - methods ; Stereoisomerism</subject><ispartof>Archives of biochemistry and biophysics, 1984-04, Vol.230 (1), p.21-29</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c241t-1b37e2070641d65f330647c3e5a31d2ac4c61febb5cec2761c753bd59295cee93</citedby><cites>FETCH-LOGICAL-c241t-1b37e2070641d65f330647c3e5a31d2ac4c61febb5cec2761c753bd59295cee93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6712232$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simpson, R.U</creatorcontrib><creatorcontrib>Wichmann, J.K</creatorcontrib><creatorcontrib>Paaren, H.E</creatorcontrib><creatorcontrib>Schnoes, H.K</creatorcontrib><creatorcontrib>Deluca, H.F</creatorcontrib><title>Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites.</description><subject>Animals</subject><subject>Biotransformation</subject><subject>Calcifediol - analogs & derivatives</subject><subject>Calcifediol - isolation & purification</subject><subject>Calcifediol - metabolism</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>Chromatography, High Pressure Liquid</subject><subject>human nutrition</subject><subject>Kidney - metabolism</subject><subject>Male</subject><subject>nutrition physiology</subject><subject>Rats</subject><subject>Spectrum Analysis - methods</subject><subject>Stereoisomerism</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptUcFO3TAQtBAVfVD-oKg-ITiY7tqJk3BDj5YiUfUAnC3HcYrbJAbbQeQ_-sFNeE-cOO1qZ3ZGO0vIZ4QzBJRfAUCwqpR4UmanFUDJGe6QFUIlGYgy2yWrN8pHsh_jHwDETPI9sicL5FzwFfn30yZd-87FnvqW8pw9TE3wL9OzS7p3A70UtJ5o0In-dc1gJ2ps10U6I2bs0hjsOXXRdzo5P1A9NNQ1dkiudWYzmkWNi-wVSkEPkWHFBh8YAvMvnr3n-Il8aHUX7eG2HpD779_u1j_Yza-r6_XFDTM8w8SwFoXlUIDMsJF5K8TcFUbYXAtsuDaZkdjaus6NNbyQaIpc1E1e8Wqe2EockOON7mPwT6ONSfUuLvfpwfoxqhJhNqpwJmYbogk-xmBb9Rhcr8OkENTyDLUkrZakVZmp12eoZe1oqz_WvW3elrbpz_iXDd5qr_Tv4KK6v-WAAnguSglS_Af9yo4j</recordid><startdate>198404</startdate><enddate>198404</enddate><creator>Simpson, R.U</creator><creator>Wichmann, J.K</creator><creator>Paaren, H.E</creator><creator>Schnoes, H.K</creator><creator>Deluca, H.F</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198404</creationdate><title>Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3</title><author>Simpson, R.U ; Wichmann, J.K ; Paaren, H.E ; Schnoes, H.K ; Deluca, H.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c241t-1b37e2070641d65f330647c3e5a31d2ac4c61febb5cec2761c753bd59295cee93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biotransformation</topic><topic>Calcifediol - analogs & derivatives</topic><topic>Calcifediol - isolation & purification</topic><topic>Calcifediol - metabolism</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Chromatography, High Pressure Liquid</topic><topic>human nutrition</topic><topic>Kidney - metabolism</topic><topic>Male</topic><topic>nutrition physiology</topic><topic>Rats</topic><topic>Spectrum Analysis - methods</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simpson, R.U</creatorcontrib><creatorcontrib>Wichmann, J.K</creatorcontrib><creatorcontrib>Paaren, H.E</creatorcontrib><creatorcontrib>Schnoes, H.K</creatorcontrib><creatorcontrib>Deluca, H.F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simpson, R.U</au><au>Wichmann, J.K</au><au>Paaren, H.E</au><au>Schnoes, H.K</au><au>Deluca, H.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1984-04</date><risdate>1984</risdate><volume>230</volume><issue>1</issue><spage>21</spage><epage>29</epage><pages>21-29</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites.</abstract><cop>United States</cop><pmid>6712232</pmid><doi>10.1016/0003-9861(84)90082-1</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1984-04, Vol.230 (1), p.21-29 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81024191 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biotransformation Calcifediol - analogs & derivatives Calcifediol - isolation & purification Calcifediol - metabolism Cells, Cultured Chickens Chromatography, High Pressure Liquid human nutrition Kidney - metabolism Male nutrition physiology Rats Spectrum Analysis - methods Stereoisomerism |
| title | Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T20%3A38%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metabolism%20of%2025-hydroxyvitamin%20D3%20by%20rat%20kidney%20cells%20in%20culture:%20isolation%20and%20identification%20of%20cis-%20and%20trans-19-nor-10-oxo-25-hydroxyvitamin%20D3&rft.jtitle=Archives%20of%20biochemistry%20and%20biophysics&rft.au=Simpson,%20R.U&rft.date=1984-04&rft.volume=230&rft.issue=1&rft.spage=21&rft.epage=29&rft.pages=21-29&rft.issn=0003-9861&rft.eissn=1096-0384&rft_id=info:doi/10.1016/0003-9861(84)90082-1&rft_dat=%3Cproquest_cross%3E81024191%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81024191&rft_id=info:pmid/6712232&rfr_iscdi=true |